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Literature summary for 2.3.1.118 extracted from

  • Saito, K.; Shinohara, A.; Kamataki, T.; Kato, R.
    N-Hydroxyarylamine O-acetyltransferase in hamster liver: identity with arylhydroxamic acid N,O-acetyltransferase and arylamine N-acetyltransferase (1986), J. Biochem., 99, 1689-1697.
    View publication on PubMed
Search for more literature for 2.3.1.118

General Stability

General Stability Organism
glycerol 50% v/v, stabilizes during storage Mesocricetus auratus

Inhibitors

Inhibitors Comment Organism Structure
1-Nitro-2-naphthol
-
 Mesocricetus auratus
2-Aminofluorene
-
 Mesocricetus auratus
iodoacetamide
-
 Mesocricetus auratus
additional information thiolactomycin does not inhibit enzyme from Syrian golden hamster Mesocricetus auratus
Pentachlorophenol
-
 Mesocricetus auratus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.27
-
 N-Hydroxy-2-acetylaminofluorene
-
 Mesocricetus auratus
0.37
-
 acetyl-CoA reaction of O-acetyl transfer to 2-hydroxyamino-6-methyldipyrido[1,2-alpha: 3',2'-d]imidazole Mesocricetus auratus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Mesocricetus auratus 5829
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
33000
-
 gel filtration Mesocricetus auratus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acetyl-CoA + an N-hydroxyarylamine Mesocricetus auratus involved in mutagenic, metabolic activation of carcinogenic arylamines to form DNA-binding species CoA + an N-acetoxyarylamine
-
 ?
acetyl-CoA + an N-hydroxyarylamine Mesocricetus auratus the enzyme from liver, but not from bacteria, can also catalyse acetylation of arylamines and N,O-acetylation of arylhydroxamates CoA + an N-acetoxyarylamine
-
 ?

Organism

Organism UniProt Comment Textmining
Mesocricetus auratus
-
 Syrian golden hamster
-

Purification (Commentary)

Purification (Comment) Organism
copurifies with arylamine acetyltransferase and arylhydroxamic acid N,O-acetyltransferase Mesocricetus auratus
purification by ammonium sulfate followed by DEAE cellulose, gel filtration on GCL-2000 and HPKB-hydroxyapatite chromatography Mesocricetus auratus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Mesocricetus auratus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.82
-
 2-hydroxyamino-6-methyldipyrido[1,2-a: 3',2'-d]imidazole as substrate Mesocricetus auratus

Storage Stability

Storage Stability Organism
-80°C, glycerol 50% v/v, stabilizes during storage Mesocricetus auratus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + 2-hydroxy-amino-6-methyldipyrido[1,2-a: 3',2'-d]imidazole
-
 Mesocricetus auratus CoA + N-acetoxy-amino-6-methyldipyrido[1,2-a: 3',2'-d]imidazole
-
 ?
acetyl-CoA + 3-hydroxyamino-1-methyl-pyrido[4,3-b]-indole
-
 Mesocricetus auratus CoA + N-acetoxy-amino-1-methyl-pyrido[4,3-b]-indole
-
 ?
acetyl-CoA + an N-hydroxyarylamine involved in mutagenic, metabolic activation of carcinogenic arylamines to form DNA-binding species Mesocricetus auratus CoA + an N-acetoxyarylamine
-
 ?
acetyl-CoA + an N-hydroxyarylamine the enzyme from liver, but not from bacteria, can also catalyse acetylation of arylamines and N,O-acetylation of arylhydroxamates Mesocricetus auratus CoA + an N-acetoxyarylamine
-
 ?
acetyl-CoA + N-hydroxy-2-acetylaminofluorene
-
 Mesocricetus auratus ?
-
 ?
acetyl-CoA + N-hydroxy-2-aminofluorene
-
 Mesocricetus auratus CoA + N-acetoxy-2-aminofluorene
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 33000 Mesocricetus auratus