BRENDA - Enzyme Database
show all sequences of 2.3.1.109

Purification and properties of a succinyltransferase from Pseudomonas aeruginosa specific for both arginine and ornithine

Tricot, C.; Vander Wauven, C.; Wattiez, R.; Falmagne, P.; Stalon, V.; Eur. J. Biochem. 224, 853-861 (1994)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
L-arginine
allosteric activator of the ornithine succinyltransferase activity
Pseudomonas aeruginosa
L-lysine
activator of the ornithine succinyltransferase activity
Pseudomonas aeruginosa
Inhibitors
Inhibitors
Commentary
Organism
Structure
agmatine
-
Pseudomonas aeruginosa
D-arginine
competitive inhibition
Pseudomonas aeruginosa
spermidine
-
Pseudomonas aeruginosa
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.04
-
L-ornithine
-
Pseudomonas aeruginosa
0.5
-
L-arginine
-
Pseudomonas aeruginosa
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
35000
-
2 * 35000 + 2 * 37000, heterotetramer, SDS-PAGE
Pseudomonas aeruginosa
37000
-
2 * 35000 + 2 * 37000, heterotetramer, SDS-PAGE
Pseudomonas aeruginosa
140000
-
PAGE
Pseudomonas aeruginosa
150000
-
gel filtration
Pseudomonas aeruginosa
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
succinyl-CoA + L-arginine
Pseudomonas aeruginosa
-
CoA + N2-succinyl-L-arginine
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Pseudomonas aeruginosa
-
-
-
Purification (Commentary)
Commentary
Organism
-
Pseudomonas aeruginosa
Storage Stability
Storage Stability
Organism
-20C, retains full activity with both substrates for several weeks, loses 10-40% of its activity following repeated freezing and thawing
Pseudomonas aeruginosa
4C, 10 mM potassium phosphate, pH 7.5, stable for 48 h
Pseudomonas aeruginosa
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
D-arginine, D-ornithine, L-lysine, L-citrulline, 2,4-aminobutyrate, 5-aminovalerate, 2-aminovalerate, agmatine or the polyamines propanediamine, putrescine, cadaverine and spermidine are not substrates
486122
Pseudomonas aeruginosa
?
-
-
-
-
succinyl-CoA + L-arginine
-
486122
Pseudomonas aeruginosa
CoA + N2-succinyl-L-arginine
-
-
-
?
succinyl-CoA + L-homoarginine
-
486122
Pseudomonas aeruginosa
CoA + N2-succinyl-L-homoarginine
-
-
-
?
succinyl-CoA + L-ornithine
-
486122
Pseudomonas aeruginosa
CoA + N2-succinyl-L-ornithine
-
-
-
?
Subunits
Subunits
Commentary
Organism
tetramer
2 * 35000 + 2 * 37000, heterotetramer, SDS-PAGE
Pseudomonas aeruginosa
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.5
-
L-homoarginine as substrate
Pseudomonas aeruginosa
8.7
-
L-arginine as substrate
Pseudomonas aeruginosa
9
-
L-ornithine as substrate
Pseudomonas aeruginosa
Ki Value [mM]
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.9
-
spermidine
-
Pseudomonas aeruginosa
2.6
-
D-arginine
-
Pseudomonas aeruginosa
3.4
-
agmatine
-
Pseudomonas aeruginosa
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
L-arginine
allosteric activator of the ornithine succinyltransferase activity
Pseudomonas aeruginosa
L-lysine
activator of the ornithine succinyltransferase activity
Pseudomonas aeruginosa
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
agmatine
-
Pseudomonas aeruginosa
D-arginine
competitive inhibition
Pseudomonas aeruginosa
spermidine
-
Pseudomonas aeruginosa
Ki Value [mM] (protein specific)
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.9
-
spermidine
-
Pseudomonas aeruginosa
2.6
-
D-arginine
-
Pseudomonas aeruginosa
3.4
-
agmatine
-
Pseudomonas aeruginosa
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.04
-
L-ornithine
-
Pseudomonas aeruginosa
0.5
-
L-arginine
-
Pseudomonas aeruginosa
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
35000
-
2 * 35000 + 2 * 37000, heterotetramer, SDS-PAGE
Pseudomonas aeruginosa
37000
-
2 * 35000 + 2 * 37000, heterotetramer, SDS-PAGE
Pseudomonas aeruginosa
140000
-
PAGE
Pseudomonas aeruginosa
150000
-
gel filtration
Pseudomonas aeruginosa
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
succinyl-CoA + L-arginine
Pseudomonas aeruginosa
-
CoA + N2-succinyl-L-arginine
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
-
Pseudomonas aeruginosa
Storage Stability (protein specific)
Storage Stability
Organism
-20C, retains full activity with both substrates for several weeks, loses 10-40% of its activity following repeated freezing and thawing
Pseudomonas aeruginosa
4C, 10 mM potassium phosphate, pH 7.5, stable for 48 h
Pseudomonas aeruginosa
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
D-arginine, D-ornithine, L-lysine, L-citrulline, 2,4-aminobutyrate, 5-aminovalerate, 2-aminovalerate, agmatine or the polyamines propanediamine, putrescine, cadaverine and spermidine are not substrates
486122
Pseudomonas aeruginosa
?
-
-
-
-
succinyl-CoA + L-arginine
-
486122
Pseudomonas aeruginosa
CoA + N2-succinyl-L-arginine
-
-
-
?
succinyl-CoA + L-homoarginine
-
486122
Pseudomonas aeruginosa
CoA + N2-succinyl-L-homoarginine
-
-
-
?
succinyl-CoA + L-ornithine
-
486122
Pseudomonas aeruginosa
CoA + N2-succinyl-L-ornithine
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
tetramer
2 * 35000 + 2 * 37000, heterotetramer, SDS-PAGE
Pseudomonas aeruginosa
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.5
-
L-homoarginine as substrate
Pseudomonas aeruginosa
8.7
-
L-arginine as substrate
Pseudomonas aeruginosa
9
-
L-ornithine as substrate
Pseudomonas aeruginosa
Other publictions for EC 2.3.1.109
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
671448
Elbahloul
Engineering the genotype of Ac ...
Acinetobacter sp.
Appl. Environ. Microbiol.
72
1410-1419
2006
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1
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4
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1
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1
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658725
Shirai
Prediction of the structure an ...
Escherichia coli
FEBS Lett.
555
505-510
2003
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1
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2
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1
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1
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1
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1
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2
1
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-
486124
Schneider
Arginine catabolism and the ar ...
Escherichia coli, Klebsiella aerogenes
J. Bacteriol.
180
4278-4286
1998
-
-
-
-
-
-
-
-
-
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2
-
4
-
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3
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3
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486123
Itoh
Cloning and characterization o ...
Aeromonas caviae, Escherichia coli, Klebsiella aerogenes, Pseudomonas aeruginosa, Salmonella enterica subsp. enterica serovar Typhimurium
J. Bacteriol.
179
7280-7290
1997
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-
2
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5
-
9
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10
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2
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5
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10
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-
486122
Tricot
Purification and properties of ...
Pseudomonas aeruginosa
Eur. J. Biochem.
224
853-861
1994
2
-
-
-
-
-
3
2
-
-
4
1
-
1
-
-
1
-
-
-
-
2
4
1
-
-
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3
-
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3
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2
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3
3
2
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4
1
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1
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2
4
1
-
-
-
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3
-
-
-
-
-
-
-
-
-
486121
Tricot
Isolation and characterization ...
Pseudomonas putida, Pseudomonas putida IRC204
J. Gen. Microbiol.
137
2911-2918
1991
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3
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6
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6
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486120
Vander Wauven
N2-Succinylornithine in ornith ...
Pseudomonas aeruginosa
Arch. Microbiol.
150
400-404
1988
-
-
1
-
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2
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3
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1
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4
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1
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2
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1
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4
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-
486119
Vander Wauven
-
Occurence of succinyl derivati ...
Burkholderia cepacia
J. Bacteriol.
11
882-886
1985
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2
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1
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1
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1
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1
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1
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