BRENDA - Enzyme Database show
show all sequences of 2.3.1.107

Expression of deacetylvindoline-4-O-acetyltransferase in Catharanthus roseus hairy roots

Magnotta, M.; Murata, J.; Chen, J.; De Luca, V.; Phytochemistry 68, 1922-1931 (2007)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expressed in Escherichia coli and Catharanthus roseus
Catharanthus roseus
Engineering
Amino acid exchange
Commentary
Organism
additional information
DAT is overexpressed in Catharanthus roseus hairy root cultures: metabolite analysis using HPLC show that transgenic hairy root extracts have an altered alkaloid profile with respect to horhammericine accumulation. It is concluded that expression of DAT in hairy roots alters their monoterpenoid indole alkaloids profile. Evidence is provided that horhammericine accumulates via a DAT interaction with the root specific minovincinine-19-O-acetyltransferase (MAT) that inhibits the MAT mediated conversion of horhammericine into 19-O-acetyl-horhammericine
Catharanthus roseus
Inhibitors
Inhibitors
Commentary
Organism
Structure
minovincinine-19-O-acetyltransferase
incubation of DAT with varying amounts of crude recombinant minovincinine-19-O-acetyltransferase (MAT) inhibits DAT activity in a dose dependent manner
Catharanthus roseus
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Catharanthus roseus
-
-
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
hair root
-
Catharanthus roseus
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
acetyl-CoA + deacetylvindoline
-
689417
Catharanthus roseus
CoA + vindoline
-
-
-
?
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
37
-
assay at
Catharanthus roseus
Cofactor
Cofactor
Commentary
Organism
Structure
CoA
-
Catharanthus roseus
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli and Catharanthus roseus
Catharanthus roseus
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
CoA
-
Catharanthus roseus
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
additional information
DAT is overexpressed in Catharanthus roseus hairy root cultures: metabolite analysis using HPLC show that transgenic hairy root extracts have an altered alkaloid profile with respect to horhammericine accumulation. It is concluded that expression of DAT in hairy roots alters their monoterpenoid indole alkaloids profile. Evidence is provided that horhammericine accumulates via a DAT interaction with the root specific minovincinine-19-O-acetyltransferase (MAT) that inhibits the MAT mediated conversion of horhammericine into 19-O-acetyl-horhammericine
Catharanthus roseus
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
minovincinine-19-O-acetyltransferase
incubation of DAT with varying amounts of crude recombinant minovincinine-19-O-acetyltransferase (MAT) inhibits DAT activity in a dose dependent manner
Catharanthus roseus
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
hair root
-
Catharanthus roseus
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
acetyl-CoA + deacetylvindoline
-
689417
Catharanthus roseus
CoA + vindoline
-
-
-
?
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
37
-
assay at
Catharanthus roseus
Other publictions for EC 2.3.1.107
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
706200
Wang
Isolation and functional analy ...
Catharanthus roseus
Plant Cell Rep.
29
185-192
2010
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1
1
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1
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710520
Shukla
Influence of cellular differen ...
Catharanthus roseus
Protoplasma
242
35-47
2010
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1
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712866
Guirimand
Spatial organization of the vi ...
Catharanthus roseus
J. Plant Physiol.
168
549-557
2010
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1
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1
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3
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1
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3
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1
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1
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684439
Campos-Tamayo
Vindoline formation in shoot c ...
Catharanthus roseus
Ann. Bot.
102
409-415
2008
1
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1
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1
8
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8
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1
1
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689417
Magnotta
Expression of deacetylvindolin ...
Catharanthus roseus
Phytochemistry
68
1922-1931
2007
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660317
Dutta
Expression of terpenoid indole ...
Catharanthus roseus
Planta
220
376-383
2005
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1
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655109
Hernandez-Dominguez
Vindoline synthesis in in vitr ...
Catharanthus roseus
Biotechnol. Lett.
26
671-674
2004
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486112
Laflamme
Molecular and biochemical anal ...
Catharanthus roseus
Plant Physiol.
125
189-198
2001
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486113
St-Pierre
Multicellular compartmentation ...
Catharanthus roseus
Plant Cell
11
887-900
1999
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4
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486114
St-Pierre
The terminal O-acetyltransfera ...
Catharanthus roseus
Plant J.
14
703-713
1998
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486108
Power
Purification and characterizat ...
Catharanthus roseus
Arch. Biochem. Biophys.
279
370-376
1990
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2
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486110
Fahn
Purification of acetyl-CoA:17- ...
Catharanthus roseus
Plant Cell Rep.
8
613-616
1990
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2
2
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2
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486109
De Luca
-
Subcellular localization of en ...
Catharanthus roseus
Plant Physiol.
85
1009-1102
1987
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486107
Fahn
Late enzymes of vindoline bios ...
Catharanthus roseus
Plant Cell Rep.
4
333-336
1985
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486111
De Luca
-
Acetyl coenzyme A:deacetylvind ...
Catharanthus roseus
J. Plant Physiol.
121
417-428
1985
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