BRENDA - Enzyme Database show
show all sequences of 2.3.1.107

Acetyl coenzyme A:deacetylvindoline O-acetyltransferase, a novel enzyme from Catharanthus

De Luca, V.; Balsevich, J.; Kurz, W.G.W.; J. Plant Physiol. 121, 417-428 (1985)
No PubMed abstract available

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
dithiothreitol
essential to maintain enzyme activity
Catharanthus roseus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0007
-
vindoline
-
Catharanthus roseus
0.0054
-
acetyl-CoA
-
Catharanthus roseus
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
45000
-
gel filtration
Catharanthus roseus
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Catharanthus roseus
-
-
-
Purification (Commentary)
Commentary
Organism
-
Catharanthus roseus
Source Tissue
Source Tissue
Commentary
Organism
Textmining
leaf
activity is highest in the youngest leaves from the uppermost apical region. The second, third and fourth leaves from the top retain 70, 50, and 25%, respectively, of the former
Catharanthus roseus
-
Storage Stability
Storage Stability
Organism
4°C, stable for several months
Catharanthus roseus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
propionyl-CoA + 17-O-deacetylvindoline
-
486111
Catharanthus roseus
CoA + 17-O-deacetyl-17-O-propionylvindoline
-
-
-
?
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
9
-
Catharanthus roseus
pH Range
pH Minimum
pH Maximum
Commentary
Organism
6.5
9.5
about 50% of maximal activity, pH 9.5: about 55% of maximal activity
Catharanthus roseus
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
dithiothreitol
essential to maintain enzyme activity
Catharanthus roseus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0007
-
vindoline
-
Catharanthus roseus
0.0054
-
acetyl-CoA
-
Catharanthus roseus
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
45000
-
gel filtration
Catharanthus roseus
Purification (Commentary) (protein specific)
Commentary
Organism
-
Catharanthus roseus
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
leaf
activity is highest in the youngest leaves from the uppermost apical region. The second, third and fourth leaves from the top retain 70, 50, and 25%, respectively, of the former
Catharanthus roseus
-
Storage Stability (protein specific)
Storage Stability
Organism
4°C, stable for several months
Catharanthus roseus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
propionyl-CoA + 17-O-deacetylvindoline
-
486111
Catharanthus roseus
CoA + 17-O-deacetyl-17-O-propionylvindoline
-
-
-
?
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
9
-
Catharanthus roseus
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
6.5
9.5
about 50% of maximal activity, pH 9.5: about 55% of maximal activity
Catharanthus roseus
Other publictions for EC 2.3.1.107
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
706200
Wang
Isolation and functional analy ...
Catharanthus roseus
Plant Cell Rep.
29
185-192
2010
-
1
1
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
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-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
1
1
1
1
-
-
710520
Shukla
Influence of cellular differen ...
Catharanthus roseus
Protoplasma
242
35-47
2010
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
5
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
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-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
5
-
-
1
-
-
-
-
-
-
-
-
-
1
1
1
1
-
-
712866
Guirimand
Spatial organization of the vi ...
Catharanthus roseus
J. Plant Physiol.
168
549-557
2010
-
-
1
-
1
-
-
-
3
-
-
1
-
3
-
-
-
-
-
1
-
-
1
1
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
1
-
-
-
-
-
3
-
-
1
-
-
-
-
-
1
-
-
1
1
-
-
-
-
-
-
-
-
-
1
1
-
-
-
684439
Campos-Tamayo
Vindoline formation in shoot c ...
Catharanthus roseus
Ann. Bot.
102
409-415
2008
1
-
-
-
-
-
1
-
-
-
-
-
-
2
-
-
-
-
-
1
8
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
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-
1
8
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
689417
Magnotta
Expression of deacetylvindolin ...
Catharanthus roseus
Phytochemistry
68
1922-1931
2007
-
-
1
-
1
-
1
-
-
-
-
-
-
3
-
-
-
-
-
1
-
-
1
-
1
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
660317
Dutta
Expression of terpenoid indole ...
Catharanthus roseus
Planta
220
376-383
2005
-
-
1
-
-
-
-
-
-
-
-
1
-
3
-
-
-
-
-
3
-
-
2
-
-
-
-
-
-
-
-
1
-
-
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-
1
1
-
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-
1
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3
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-
2
-
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-
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-
-
-
-
-
-
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-
-
-
-
655109
Hernandez-Dominguez
Vindoline synthesis in in vitr ...
Catharanthus roseus
Biotechnol. Lett.
26
671-674
2004
1
-
-
-
-
-
-
-
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1
-
2
-
-
-
-
-
2
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
1
-
-
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-
-
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-
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-
1
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-
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-
2
-
-
2
-
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-
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-
-
-
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-
-
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-
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-
486112
Laflamme
Molecular and biochemical anal ...
Catharanthus roseus
Plant Physiol.
125
189-198
2001
-
-
1
-
-
-
-
2
-
-
-
-
-
3
-
-
1
-
-
5
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
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2
-
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-
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-
1
-
5
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
486113
St-Pierre
Multicellular compartmentation ...
Catharanthus roseus
Plant Cell
11
887-900
1999
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
4
-
-
1
-
-
-
-
-
-
-
-
-
-
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-
-
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-
-
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1
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4
-
-
1
-
-
-
-
-
-
-
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-
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-
-
-
-
486114
St-Pierre
The terminal O-acetyltransfera ...
Catharanthus roseus
Plant J.
14
703-713
1998
-
-
1
-
-
-
-
-
-
-
2
1
-
1
-
-
1
-
-
2
-
-
1
1
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-
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-
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1
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2
1
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1
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2
-
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1
1
-
-
-
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-
-
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-
-
-
-
-
-
-
486108
Power
Purification and characterizat ...
Catharanthus roseus
Arch. Biochem. Biophys.
279
370-376
1990
-
-
-
-
-
-
5
2
-
-
-
1
-
2
-
-
1
-
-
1
1
-
2
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
5
-
2
-
-
-
1
-
-
-
1
-
1
1
-
2
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
486110
Fahn
Purification of acetyl-CoA:17- ...
Catharanthus roseus
Plant Cell Rep.
8
613-616
1990
-
-
-
-
-
-
2
2
-
-
2
1
-
1
-
-
1
-
-
1
1
-
2
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
2
1
2
-
-
2
1
-
-
-
1
-
1
1
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
486109
De Luca
-
Subcellular localization of en ...
Catharanthus roseus
Plant Physiol.
85
1009-1102
1987
-
-
-
-
-
-
-
-
1
-
-
1
-
1
-
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-
-
-
1
-
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2
-
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1
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1
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1
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2
-
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-
-
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-
-
-
-
486107
Fahn
Late enzymes of vindoline bios ...
Catharanthus roseus
Plant Cell Rep.
4
333-336
1985
-
-
-
-
-
-
-
-
-
1
-
1
-
1
-
-
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-
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2
-
-
2
-
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1
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1
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2
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2
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-
-
-
-
486111
De Luca
-
Acetyl coenzyme A:deacetylvind ...
Catharanthus roseus
J. Plant Physiol.
121
417-428
1985
1
-
-
-
-
-
-
2
-
-
1
-
-
1
-
-
1
-
-
1
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1
1
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1
1
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1
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2
-
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1
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1
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1
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1
1
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1
1
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