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show all sequences of 2.3.1.102

Isolation and properties of N epsilon-hydroxylysine:acetyl coenzyme A nepsilon-transacetylase from Escherichia coli pABN11

Coy, M.; Paw, B.H.; Bindereif, A.; Neilands, J.B.; Biochemistry 25, 2485-2489 (1986)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
Coomassie brilliant blue
mixed inhibitor
Escherichia coli
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.043
-
acetyl-CoA
+ L-N6-hydroxylysine
Escherichia coli
0.055
-
L-N6-hydroxylysine
-
Escherichia coli
0.079
-
acetyl-CoA
+ L-N5-hydroxyornithine
Escherichia coli
0.099
-
acetyl-CoA
+ 1-amino-5-(hydroxyamino)pentane
Escherichia coli
0.101
-
acetyl-CoA
+ N-methylhydroxylamine
Escherichia coli
0.317
-
L-N5-hydroxyornithine
-
Escherichia coli
0.33
-
1-amino-5-(hydroxyamino)pentane
-
Escherichia coli
0.396
-
N-Methylhydroxylamine
-
Escherichia coli
1.81
-
acetyl-CoA
+ hydroxylamine
Escherichia coli
329.4
-
hydroxylamine
-
Escherichia coli
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
33000
-
x * 33000, SDS-PAGE
Escherichia coli
150000
200000
gel filtration, native enzyme
Escherichia coli
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
additional information
Escherichia coli
enzyme is involved in the biosynthesis of aerobactin in E. coli
?
-
-
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Escherichia coli
-
294, with plasmid pABN11
-
Purification (Commentary)
Commentary
Organism
with plasmid pABN11
Escherichia coli
Specific Activity [micromol/min/mg]
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
26
-
N6-hydroxylysine
Escherichia coli
Storage Stability
Storage Stability
Organism
20C, stable for several days
Escherichia coli
4C, stable for several months
Escherichia coli
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
acetyl-CoA + (5-hydroxyamine)pentan-1-amine
-
486098
Escherichia coli
CoA + N-(5-aminopentyl)-N-hydroxyacetamide
-
-
-
?
acetyl-CoA + DL-N6-hydroxylysine
-
486098
Escherichia coli
CoA + N6-acetyl-N6-hydroxylysine
-
-
-
?
acetyl-CoA + hydroxylamine
-
486098
Escherichia coli
CoA + N-acetylhydroxylamine
-
-
-
?
acetyl-CoA + L-N5-hydroxyornithine
-
486098
Escherichia coli
CoA + N5-acetyl-N5-hydroxyornithine
-
-
-
?
acetyl-CoA + L-N6-hydroxylysine
preferred substrate
486098
Escherichia coli
CoA + N6-acetyl-N6-hydroxylysine
-
486098
Escherichia coli
?
acetyl-CoA + N-methylhydroxylamine
-
486098
Escherichia coli
CoA + N-acetyl-N-methylhydroxylamine
-
-
-
?
additional information
propionyl-CoA has 5% of activity compared to acetyl-CoA
486098
Escherichia coli
?
-
-
-
-
additional information
enzyme is involved in the biosynthesis of aerobactin in E. coli
486098
Escherichia coli
?
-
-
-
-
Subunits
Subunits
Commentary
Organism
?
x * 33000, SDS-PAGE
Escherichia coli
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
40
-
sharp decline above
Escherichia coli
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.0000385
-
N-Methylhydroxylamine
-
Escherichia coli
0.0000477
-
N6-hydroxylysine
-
Escherichia coli
0.0000797
-
1-amino-5(hydroxyamino)pentane
-
Escherichia coli
0.000112
-
N6-Hydroxyornithine
-
Escherichia coli
0.00177
-
hydroxylamine
-
Escherichia coli
Ki Value [mM]
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.00071
-
Coomassie brilliant blue
-
Escherichia coli
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Coomassie brilliant blue
mixed inhibitor
Escherichia coli
Ki Value [mM] (protein specific)
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.00071
-
Coomassie brilliant blue
-
Escherichia coli
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.043
-
acetyl-CoA
+ L-N6-hydroxylysine
Escherichia coli
0.055
-
L-N6-hydroxylysine
-
Escherichia coli
0.079
-
acetyl-CoA
+ L-N5-hydroxyornithine
Escherichia coli
0.099
-
acetyl-CoA
+ 1-amino-5-(hydroxyamino)pentane
Escherichia coli
0.101
-
acetyl-CoA
+ N-methylhydroxylamine
Escherichia coli
0.317
-
L-N5-hydroxyornithine
-
Escherichia coli
0.33
-
1-amino-5-(hydroxyamino)pentane
-
Escherichia coli
0.396
-
N-Methylhydroxylamine
-
Escherichia coli
1.81
-
acetyl-CoA
+ hydroxylamine
Escherichia coli
329.4
-
hydroxylamine
-
Escherichia coli
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
33000
-
x * 33000, SDS-PAGE
Escherichia coli
150000
200000
gel filtration, native enzyme
Escherichia coli
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
additional information
Escherichia coli
enzyme is involved in the biosynthesis of aerobactin in E. coli
?
-
-
-
Purification (Commentary) (protein specific)
Commentary
Organism
with plasmid pABN11
Escherichia coli
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
26
-
N6-hydroxylysine
Escherichia coli
Storage Stability (protein specific)
Storage Stability
Organism
20C, stable for several days
Escherichia coli
4C, stable for several months
Escherichia coli
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
acetyl-CoA + (5-hydroxyamine)pentan-1-amine
-
486098
Escherichia coli
CoA + N-(5-aminopentyl)-N-hydroxyacetamide
-
-
-
?
acetyl-CoA + DL-N6-hydroxylysine
-
486098
Escherichia coli
CoA + N6-acetyl-N6-hydroxylysine
-
-
-
?
acetyl-CoA + hydroxylamine
-
486098
Escherichia coli
CoA + N-acetylhydroxylamine
-
-
-
?
acetyl-CoA + L-N5-hydroxyornithine
-
486098
Escherichia coli
CoA + N5-acetyl-N5-hydroxyornithine
-
-
-
?
acetyl-CoA + L-N6-hydroxylysine
preferred substrate
486098
Escherichia coli
CoA + N6-acetyl-N6-hydroxylysine
-
486098
Escherichia coli
?
acetyl-CoA + N-methylhydroxylamine
-
486098
Escherichia coli
CoA + N-acetyl-N-methylhydroxylamine
-
-
-
?
additional information
propionyl-CoA has 5% of activity compared to acetyl-CoA
486098
Escherichia coli
?
-
-
-
-
additional information
enzyme is involved in the biosynthesis of aerobactin in E. coli
486098
Escherichia coli
?
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 33000, SDS-PAGE
Escherichia coli
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
40
-
sharp decline above
Escherichia coli
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.0000385
-
N-Methylhydroxylamine
-
Escherichia coli
0.0000477
-
N6-hydroxylysine
-
Escherichia coli
0.0000797
-
1-amino-5(hydroxyamino)pentane
-
Escherichia coli
0.000112
-
N6-Hydroxyornithine
-
Escherichia coli
0.00177
-
hydroxylamine
-
Escherichia coli
Other publictions for EC 2.3.1.102
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
486098
Coy
Isolation and properties of N ...
Escherichia coli
Biochemistry
25
2485-2489
1986
-
-
-
-
-
-
1
10
-
-
2
1
-
1
-
-
1
-
-
-
1
2
8
1
1
-
-
5
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
1
10
-
-
2
1
-
-
-
1
-
-
1
2
8
1
1
-
-
5
-
-
-
-
-
-
-
-
-
-
486099
Lorenzo de
Aerobactin biosynthesis and tr ...
Escherichia coli
J. Bacteriol.
165
570-578
1986
-
-
1
-
-
-
-
-
-
-
1
1
-
1
-
-
1
-
-
-
-
-
1
-
1
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
1
-
-
-
-
1
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-