BRENDA - Enzyme Database show
show all sequences of 2.3.1.101

A reversed genetic approach reveals the coenzyme specificity and other catalytic properties of three enzymes putatively involved in anaerobic oxidation of methane with sulfate

Kojima, H.; Moll, J.; Kahnt, J.; Fukui, M.; Shima, S.; Environ. Microbiol. 16, 3431-3442 (2014)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expression in Escherichia coli
uncultured archaeon
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.01
-
formylmethanofuran
pH 6.0, 10C
uncultured archaeon
0.08
-
5,6,7,8-tetrahydromethanopterin
pH 6.0, 10C
uncultured archaeon
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
31864
-
x * 31864, calculated from sequence
uncultured archaeon
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
formylmethanofuran + 5,6,7,8-tetrahydromethanopterin
uncultured archaeon
-
methanofuran + 5-formyl-5,6,7,8-tetrahydromethanopterin
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
uncultured archaeon
D1J8Z9
-
-
Purification (Commentary)
Commentary
Organism
-
uncultured archaeon
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
formylmethanofuran + 5,6,7,8-tetrahydromethanopterin
-
727375
uncultured archaeon
methanofuran + 5-formyl-5,6,7,8-tetrahydromethanopterin
-
-
-
?
formylmethanofuran + 5,6,7,8-tetrahydromethanopterin
the reverse reaction is difficult to measure because the free-energy change of the reverse direction is close to equilibrium but endergonic. Therefore, the reverse reaction stopps almost as soon as a small amount of substrate is converted to product
727375
uncultured archaeon
methanofuran + 5-formyl-5,6,7,8-tetrahydromethanopterin
-
-
-
?
Subunits
Subunits
Commentary
Organism
?
x * 31864, calculated from sequence
uncultured archaeon
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
40
-
-
uncultured archaeon
Temperature Range [C]
Temperature Minimum [C]
Temperature Maximum [C]
Commentary
Organism
20
50
20C: about 60% of maximal activity, 50C: about 60% of maximal activity
uncultured archaeon
pI Value
Organism
Commentary
pI Value Maximum
pI Value
uncultured archaeon
calculated from sequence
-
4.7
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Escherichia coli
uncultured archaeon
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.01
-
formylmethanofuran
pH 6.0, 10C
uncultured archaeon
0.08
-
5,6,7,8-tetrahydromethanopterin
pH 6.0, 10C
uncultured archaeon
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
31864
-
x * 31864, calculated from sequence
uncultured archaeon
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
formylmethanofuran + 5,6,7,8-tetrahydromethanopterin
uncultured archaeon
-
methanofuran + 5-formyl-5,6,7,8-tetrahydromethanopterin
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
-
uncultured archaeon
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
formylmethanofuran + 5,6,7,8-tetrahydromethanopterin
-
727375
uncultured archaeon
methanofuran + 5-formyl-5,6,7,8-tetrahydromethanopterin
-
-
-
?
formylmethanofuran + 5,6,7,8-tetrahydromethanopterin
the reverse reaction is difficult to measure because the free-energy change of the reverse direction is close to equilibrium but endergonic. Therefore, the reverse reaction stopps almost as soon as a small amount of substrate is converted to product
727375
uncultured archaeon
methanofuran + 5-formyl-5,6,7,8-tetrahydromethanopterin
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 31864, calculated from sequence
uncultured archaeon
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
40
-
-
uncultured archaeon
Temperature Range [C] (protein specific)
Temperature Minimum [C]
Temperature Maximum [C]
Commentary
Organism
20
50
20C: about 60% of maximal activity, 50C: about 60% of maximal activity
uncultured archaeon
pI Value (protein specific)
Organism
Commentary
pI Value Maximum
pI Value
uncultured archaeon
calculated from sequence
-
4.7
Other publictions for EC 2.3.1.101
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
727375
Kojima
A reversed genetic approach re ...
uncultured archaeon
Environ. Microbiol.
16
3431-3442
2014
-
-
1
-
-
-
-
2
-
-
1
1
-
3
-
-
1
-
-
-
-
-
2
1
1
1
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
-
-
-
2
-
-
1
1
-
-
-
1
-
-
-
-
2
1
1
1
-
-
-
-
-
1
-
-
-
-
-
-
675376
Acharya
The structure of formylmethano ...
Methanopyrus kandleri
J. Mol. Biol.
357
870-879
2006
-
-
-
1
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
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-
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-
1
-
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-
-
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-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
661043
Shima
Hyperthermophilic and salt-dep ...
Methanopyrus kandleri
Biochem. Soc. Trans.
32
269-272
2004
1
-
-
-
-
1
-
-
-
2
1
-
-
4
-
-
-
-
-
1
-
-
-
3
-
-
2
-
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-
1
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1
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2
1
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-
1
-
-
-
3
-
-
2
-
-
-
-
-
-
-
-
-
-
-
486096
Pomper
Generation of formate by the f ...
Methylorubrum extorquens
FEBS Lett.
523
133-137
2002
-
-
-
-
-
-
-
-
-
-
-
1
-
2
-
-
-
1
-
-
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-
2
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1
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-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
726443
Mamat
Crystal structures and enzymat ...
Archaeoglobus fulgidus, Methanopyrus kandleri, Methanopyrus kandleri DSM 6324, Methanosarcina barkeri, Methanosarcina barkeri DSM 804
Protein Sci.
11
:2168-2178
2002
-
-
2
2
-
-
-
-
-
3
-
-
-
9
-
-
-
-
-
-
-
-
5
4
-
-
3
-
-
-
-
-
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-
-
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2
-
2
-
-
-
-
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-
3
-
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-
-
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-
-
-
5
4
-
-
3
-
-
-
-
-
-
-
-
-
-
-
486095
Pomper
Characterization of the formyl ...
Methylorubrum extorquens, Methylorubrum extorquens ATCC 14718 / DSM 1338 / JCM 2805 / NCIMB 9133 / AM1
Eur. J. Biochem.
268
4769-4775
2001
-
-
1
-
-
-
-
2
-
4
1
2
-
21
1
-
1
1
-
-
1
-
2
1
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
2
-
4
1
2
-
1
-
1
-
-
1
-
2
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
486097
Shima
Tetrahydromethanopterin-specif ...
Methanopyrus kandleri
Methods Enzymol.
331
317-353
2001
-
-
1
-
-
-
-
2
-
1
1
-
1
1
1
-
1
-
-
-
1
-
1
1
1
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
2
-
1
1
-
1
1
-
1
-
-
1
-
1
1
1
-
1
-
-
-
-
-
-
-
-
-
-
-
726828
Shima
Activation and thermostabiliza ...
Methanopyrus kandleri
Arch. Microbiol.
170
469-472
1998
1
-
-
-
-
1
-
-
-
-
-
-
-
2
1
-
-
-
-
-
-
-
1
-
1
-
2
-
1
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-
1
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-
1
-
-
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-
-
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-
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1
-
-
-
-
-
-
1
-
1
-
2
-
1
-
-
-
-
-
-
-
-
-
486094
Ermler
Formylmethanofuran: tetrahydro ...
Methanopyrus kandleri
Structure
5
635-646
1997
-
-
-
1
-
-
-
-
-
-
-
1
-
3
-
-
-
-
-
-
-
-
2
1
-
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1
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-
-
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-
-
1
-
-
-
-
-
-
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-
-
1
-
-
-
-
-
-
-
-
2
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
721443
Vorholt
Pathways of autotrophic CO2 fi ...
Ferroglobus placidus, Ferroglobus placidus DSM 10642
Arch. Microbiol.
167
19-23
1997
-
-
-
-
-
-
-
-
-
-
-
-
-
5
-
-
-
-
-
-
1
-
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1
-
-
-
1
-
-
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-
-
1
-
-
-
1
-
-
-
1
-
-
-
-
1
1
-
-
-
486092
Kunow
Primary structure and properti ...
Methanosarcina barkeri, Methanosarcina barkeri Fusaro / DSM 804
Arch. Microbiol.
165
97-105
1996
-
-
-
-
-
-
-
-
-
-
1
2
-
8
-
-
1
-
-
-
1
-
2
1
1
-
1
-
-
-
-
-
-
-
-
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-
-
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-
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-
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1
2
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1
-
-
1
-
2
1
1
-
1
-
-
-
-
-
-
-
-
-
-
-
486093
Shima
Crystallization and preliminar ...
Methanopyrus kandleri
Proteins Struct. Funct. Genet.
26
118-120
1996
-
-
-
1
-
-
-
-
-
-
-
1
-
3
-
-
-
-
-
-
-
-
1
-
-
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-
-
-
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1
-
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-
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-
1
-
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-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
486091
Shima
Formylmethanofuran:tetrahydrom ...
Methanopyrus kandleri
Eur. J. Biochem.
230
906-913
1995
-
-
1
-
-
-
-
4
-
1
1
-
-
7
-
-
1
-
-
-
1
-
1
1
1
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
4
-
1
1
-
-
-
-
1
-
-
1
-
1
1
1
-
1
-
-
-
-
-
-
-
-
-
-
-
721441
Vornolt
-
Enzymes and coenzymes of the c ...
Archaeoglobus fulgidus, Archaeoglobus lithotrophicus, Archaeoglobus lithotrophicus TF-2, Archaeoglobus profundus, Archaeoglobus profundus DSM 5631
Arch. Microbiol.
163
112-118
1995
-
-
-
-
-
-
-
-
-
-
-
-
-
5
-
-
-
-
-
3
3
-
-
-
3
-
-
-
3
-
-
-
-
-
-
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-
-
-
-
-
-
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-
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-
-
-
-
-
-
-
-
-
-
3
3
-
-
-
3
-
-
-
3
-
-
-
-
3
3
-
-
-
725983
Lehmacher
Cloning, sequencing and transc ...
Methanothermus fervidus, Methanothermus fervidus DSM 2088
Mol. Gen. Genet.
242
73-80
1994
-
-
1
-
-
-
-
-
-
-
2
-
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6
-
-
1
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1
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1
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2
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1
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1
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-
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-
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-
-
-
-
486086
Schwrer
Formylmethanofuran:tetrahydrom ...
Archaeoglobus fulgidus, Archaeoglobus fulgidus VC-19 / DSM 4304
Arch. Microbiol.
159
225-232
1993
-
-
-
-
-
1
-
2
-
2
2
-
1
4
1
-
1
1
-
-
1
-
2
1
1
-
2
-
1
-
-
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1
-
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2
-
2
2
-
1
1
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1
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1
-
2
1
1
-
2
-
1
-
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-
-
-
-
-
-
-
486087
Breitung
Salt dependence, kinetic prope ...
Methanopyrus kandleri
Eur. J. Biochem.
210
971-981
1992
-
-
-
-
-
1
-
3
-
3
1
-
1
5
-
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1
1
-
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1
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2
1
1
-
1
-
1
-
-
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1
-
-
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3
-
3
1
-
1
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1
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1
-
2
1
1
-
1
-
1
-
-
-
-
-
-
-
-
-
11925
Breitung
N-Furfurylformamide as a pseud ...
Methanosarcina barkeri, Methanosarcina barkeri Fusaro / DSM 804, Methanothermobacter thermautotrophicus
FEBS Lett.
268
257-260
1990
-
-
-
-
-
-
-
4
-
-
-
-
-
14
-
-
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-
-
-
-
-
7
-
-
-
-
-
-
-
-
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4
-
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-
7
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
486088
DiMarco
The formylmethanofuran: tetrah ...
Methanothermobacter thermautotrophicus
J. Biol. Chem.
265
472-476
1990
-
-
1
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
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1
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1
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1
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-
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-
-
-
-
486089
Breitung
Formylmethanofuran: tetrahydro ...
Methanosarcina barkeri, Methanothermobacter thermautotrophicus
FEBS Lett.
275
226-230
1990
-
-
-
-
-
-
-
5
-
-
2
2
-
4
1
-
2
2
-
-
1
1
4
2
1
-
1
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
5
-
-
2
2
-
1
-
2
-
-
1
1
4
2
1
-
1
2
1
-
-
-
-
-
-
-
-
-
486084
Donnelly
-
The role of formylmethanofuran ...
Methanothermobacter thermautotrophicus
J. Biol. Chem.
261
16652-16659
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