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Literature summary for 2.3.1.101 extracted from
- Primary structure and properties of the formyltransferase from the mesophilic Methanosarcina barkeri. Comparison with the enzymes from thermophilic and hyperthermophilic methanogens (1996), Arch. Microbiol., 165, 97-105.
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 32000 | - |
1 * 32000 SDS-PAGE | Methanosarcina barkeri |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| formylmethanofuran + 5,6,7,8-tetrahydromethanopterin | Methanosarcina barkeri | - |
methanofuran + N5-formyl-5,6,7,8-tetrahydromethanopterin | - |
r |  |
| formylmethanofuran + 5,6,7,8-tetrahydromethanopterin | Methanosarcina barkeri Fusaro / DSM 804 | - |
methanofuran + N5-formyl-5,6,7,8-tetrahydromethanopterin | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Methanosarcina barkeri | P55301 | - |
- |
| Methanosarcina barkeri Fusaro / DSM 804 | P55301 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme | Methanosarcina barkeri |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Methanosarcina barkeri |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| formylmethanofuran + 5,6,7,8-tetrahydromethanopterin | - |
Methanosarcina barkeri | methanofuran + N5-formyl-5,6,7,8-tetrahydromethanopterin | - |
r | |
| formylmethanofuran + 5,6,7,8-tetrahydromethanopterin | - |
Methanosarcina barkeri Fusaro / DSM 804 | methanofuran + N5-formyl-5,6,7,8-tetrahydromethanopterin | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 32000 SDS-PAGE | Methanosarcina barkeri |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 65 | - |
- |
Methanosarcina barkeri |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
up to, in presence of 1.5 M K2HPO4, hyperthermophilic enzyme | Methanosarcina barkeri |
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