Salt dependence, kinetic properties and catalytic mechanism of N-formylmethanofuran:tetrahydromethanopterin formyltransferase from the extreme thermophile Methanopyrus kandleri

Breitung, J.; Börner, G.; Scholz, S.; Linder, D.; Stetter, K.O.; Thauer, R.K.; Eur. J. Biochem. 210, 971-981 (1992)

Data extracted from this reference:

KM Value [mM]

KM Value Maximum [mM]

Substrate

Commentary

Organism

Structure

0.05

-

formylmethanofuran

-

Methanopyrus kandleri

0.1

-

5,6,7,8-tetrahydromethanopterin

-

Methanopyrus kandleri

20

-

N-Furfurylformamide

-

Methanopyrus kandleri

Metals/Ions

Commentary

Organism

Structure

(NH4)2SO4

-

Methanopyrus kandleri

K2HPO4

no activity in absence, assay mixture contains 2 M

Methanopyrus kandleri

salts

absolutely dependent on the presence of phosphate or sulfate salts for activity, efficiency of activation in decreasing order: K2HPO4, (NH4)2SO4, K2SO4, Na2SO4, Na2HPO4, no activation: NaCl, KCl, NH4Cl

Methanopyrus kandleri

Reaction

Commentary

Organism

Reaction ID

formylmethanofuran + 5,6,7,8-tetrahydromethanopterin = methanofuran + 5-formyl-5,6,7,8-tetrahydromethanopterin

ternary complex type mechanism

Methanopyrus kandleri

Substrates

Commentary Substrates

Literature (Substrates)

Organism

Products

Commentary (Products)

Literature (Products)

Organism (Products)

Reversibility

Substrate Product ID

formylmethanofuran + 5,6,7,8-tetrahydromethanopterin

specific for tetrahydromethanopterin

486087

Methanopyrus kandleri

methanofuran + N5-formyl-5,6,7,8-tetrahydromethanopterin

-

486087

Methanopyrus kandleri

-

N-furfurylformamide + 5,6,7,8-tetrahydromethanopterin

pseudo-substrate

486087

Methanopyrus kandleri

2-(aminomethyl)furane + N5-formyl-5,6,7,8-tetrahydromethanopterin

-

-

-

?

KM Value [mM]

KM Value Maximum [mM]

Substrate

Commentary

Organism

Structure

0.05

-

formylmethanofuran

-

Methanopyrus kandleri

0.1

-

5,6,7,8-tetrahydromethanopterin

-

Methanopyrus kandleri

20

-

N-Furfurylformamide

-

Methanopyrus kandleri

Metals/Ions

Commentary

Organism

Structure

(NH4)2SO4

-

Methanopyrus kandleri

K2HPO4

no activity in absence, assay mixture contains 2 M

Methanopyrus kandleri

salts

absolutely dependent on the presence of phosphate or sulfate salts for activity, efficiency of activation in decreasing order: K2HPO4, (NH4)2SO4, K2SO4, Na2SO4, Na2HPO4, no activation: NaCl, KCl, NH4Cl

Methanopyrus kandleri

Substrates

Commentary Substrates

Literature (Substrates)

Organism

Products

Commentary (Products)

Literature (Products)

Organism (Products)

Reversibility

ID

formylmethanofuran + 5,6,7,8-tetrahydromethanopterin

specific for tetrahydromethanopterin

486087

Methanopyrus kandleri

methanofuran + N5-formyl-5,6,7,8-tetrahydromethanopterin

-

486087

Methanopyrus kandleri

-

N-furfurylformamide + 5,6,7,8-tetrahydromethanopterin

pseudo-substrate

486087

Methanopyrus kandleri

2-(aminomethyl)furane + N5-formyl-5,6,7,8-tetrahydromethanopterin

-

-

-

?

No.

1st author

Pub Med

title

organims

journal

volume

pages

year

Activating Compound

Application

Cloned(Commentary)

Crystallization (Commentary)

Engineering

General Stability

Inhibitors

KM Value [mM]

Localization

Metals/Ions

Molecular Weight [Da]

Natural Substrates/ Products (Substrates)

Organic Solvent Stability

Organism

Oxidation Stability

Posttranslational Modification

Purification (Commentary)

Reaction

Renatured (Commentary)

Source Tissue

Specific Activity [micromol/min/mg]

Storage Stability

Substrates and Products (Substrate)

Subunits

Synonyms

Temperature Optimum [°C]

Temperature Range [°C]

Temperature Stability [°C]

Turnover Number [1/s]

pH Optimum

pH Range

pH Stability

Cofactor

Ki Value [mM]

pI Value

IC50 Value

Activating Compound (protein specific)

Application (protein specific)

Cloned(Commentary) (protein specific)

Cofactor (protein specific)

Crystallization (Commentary) (protein specific)

Engineering (protein specific)

General Stability (protein specific)

IC50 Value (protein specific)

Inhibitors (protein specific)

Ki Value [mM] (protein specific)

KM Value [mM] (protein specific)

Localization (protein specific)

Metals/Ions (protein specific)

Molecular Weight [Da] (protein specific)

Natural Substrates/ Products (Substrates) (protein specific)

Organic Solvent Stability (protein specific)

Oxidation Stability (protein specific)

Posttranslational Modification (protein specific)

Purification (Commentary) (protein specific)

Renatured (Commentary) (protein specific)

Source Tissue (protein specific)

Specific Activity [micromol/min/mg] (protein specific)

Storage Stability (protein specific)

Substrates and Products (Substrate) (protein specific)

Subunits (protein specific)

Temperature Optimum [°C] (protein specific)

Temperature Range [°C] (protein specific)

Temperature Stability [°C] (protein specific)

Turnover Number [1/s] (protein specific)

pH Optimum (protein specific)

pH Range (protein specific)

pH Stability (protein specific)

pI Value (protein specific)

Expression

General Information

General Information (protein specific)

Expression (protein specific)

KCat/KM [mM/s]

KCat/KM [mM/s] (protein specific)

727375

Kojima

A reversed genetic approach re ...

uncultured archaeon

Environ. Microbiol.

16

3431-3442

2014

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675376

Acharya

The structure of formylmethano ...

Methanopyrus kandleri

J. Mol. Biol.

357

870-879

2006

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661043

Shima

Hyperthermophilic and salt-dep ...

Methanopyrus kandleri

Biochem. Soc. Trans.

32

269-272

2004

1

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486096

Pomper

Generation of formate by the f ...

Methylorubrum extorquens

FEBS Lett.

523

133-137

2002

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726443

Mamat

Crystal structures and enzymat ...

Archaeoglobus fulgidus, Methanopyrus kandleri, Methanopyrus kandleri DSM 6324, Methanosarcina barkeri, Methanosarcina barkeri DSM 804

Protein Sci.

11

:2168-2178

2002

-

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2

2

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3

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9

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486095

Pomper

Characterization of the formyl ...

Methylorubrum extorquens, Methylorubrum extorquens ATCC 14718 / DSM 1338 / JCM 2805 / NCIMB 9133 / AM1

Eur. J. Biochem.

268

4769-4775

2001

-

-

1

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2

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4

1

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22

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-

-

-

-

486097

Shima

Tetrahydromethanopterin-specif ...

Methanopyrus kandleri

Methods Enzymol.

331

317-353

2001

-

-

1

-

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2

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1

1

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-

726828

Shima

Activation and thermostabiliza ...

Methanopyrus kandleri

Arch. Microbiol.

170

469-472

1998

1

-

-

-

-

1

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2

1

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1

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-

486094

Ermler

Formylmethanofuran: tetrahydro ...

Methanopyrus kandleri

Structure

5

635-646

1997

-

-

-

1

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1

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3

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721443

Vorholt

Pathways of autotrophic CO2 fi ...

Ferroglobus placidus, Ferroglobus placidus DSM 10642

Arch. Microbiol.

167

19-23

1997

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5

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486092

Kunow

Primary structure and properti ...

Methanosarcina barkeri, Methanosarcina barkeri Fusaro / DSM 804

Arch. Microbiol.

165

97-105

1996

-

-

-

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2

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7

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486093

Shima

Crystallization and preliminar ...

Methanopyrus kandleri

Proteins Struct. Funct. Genet.

26

118-120

1996

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486091

Shima

Formylmethanofuran:tetrahydrom ...

Methanopyrus kandleri

Eur. J. Biochem.

230

906-913

1995

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1

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7

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721441

Vornolt

-

Enzymes and coenzymes of the c ...

Archaeoglobus fulgidus, Archaeoglobus lithotrophicus, Archaeoglobus lithotrophicus TF-2, Archaeoglobus profundus, Archaeoglobus profundus DSM 5631

Arch. Microbiol.

163

112-118

1995

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725983

Lehmacher

Cloning, sequencing and transc ...

Methanothermus fervidus, Methanothermus fervidus DSM 2088

Mol. Gen. Genet.

242

73-80

1994

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6

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486086

Schwörer

Formylmethanofuran:tetrahydrom ...

Archaeoglobus fulgidus, Archaeoglobus fulgidus VC-19 / DSM 4304

Arch. Microbiol.

159

225-232

1993

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-

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486087

Breitung

Salt dependence, kinetic prope ...

Methanopyrus kandleri

Eur. J. Biochem.

210

971-981

1992

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11925

Breitung

N-Furfurylformamide as a pseud ...

Methanosarcina barkeri, Methanosarcina barkeri Fusaro / DSM 804, Methanothermobacter thermautotrophicus

FEBS Lett.

268

257-260

1990

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486088

DiMarco

The formylmethanofuran: tetrah ...

Methanothermobacter thermautotrophicus

J. Biol. Chem.

265

472-476

1990

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486089

Breitung

Formylmethanofuran: tetrahydro ...

Methanosarcina barkeri, Methanothermobacter thermautotrophicus

FEBS Lett.

275

226-230

1990

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5

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1

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486084

Donnelly

-

The role of formylmethanofuran ...

Methanothermobacter thermautotrophicus

J. Biol. Chem.

261

16652-16659

1986

-

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486085

Leigh

-

Structure of methanofuran, the ...

Methanothermobacter thermautotrophicus

J. Am. Chem. Soc.

106

3636-3640

1984

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