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Literature summary for 2.3.1.100 extracted from

  • Bizzozero, O.A.; McGarry, J.F.; Lees, M.B.
    Acylation of endogenous myelin proteolipid protein with different acyl-CoAs (1987), J. Biol. Chem., 262, 2138-2145.
    View publication on PubMed
Search for more literature for 2.3.1.100

Activating Compound

Activating Compound Comment Organism Structure
dithiothreitol 1 mM, slightly stimulates Rattus norvegicus
Nonidet P-40 stimulates at 0.05% Rattus norvegicus
octyl glucoside slight stimulation Rattus norvegicus
sodium deoxycholate stimulates at 0.5% Rattus norvegicus
Triton X-100 0.1%, stimulates Rattus norvegicus
Tween 20 0.05%, slight stimulation Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
Sodium dodecyl sulfate at concentration 0.5%: 92% loss of activity Rattus norvegicus
Tween 20 inhibits slightly at 0.5% Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.041
-
 palmitoyl-CoA
-
 Rattus norvegicus
0.046
-
 oleoyl-CoA
-
 Rattus norvegicus
0.05
-
 stearoyl-CoA
-
 Rattus norvegicus
0.219
-
 myristoyl-CoA
-
 Rattus norvegicus

Metals/Ions

Metals/Ions Comment Organism Structure
MgCl2 2 mM, stimulates Rattus norvegicus
MnCl2 stimulates Rattus norvegicus
NaF slight stimulation Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Rattus norvegicus enzyme is probably involved in the acylation of endogenous myelin proteolipid protein ?
-
 ?
palmitoyl-CoA + myelin proteolipid protein Rattus norvegicus
-
 CoA + myelin proteolipid O-palmitoylprotein
-
 ?

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
brain myelin labeled product also found in vitro in brain tissue slices Rattus norvegicus
-

Storage Stability

Storage Stability Organism
freezing and thawing causes marked decrease in activity Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information enzyme is probably involved in the acylation of endogenous myelin proteolipid protein Rattus norvegicus ?
-
 ?
myristoyl-CoA + myelin proteolipid protein low activity Rattus norvegicus CoA + myelin proteolipid O-myristoylprotein
-
 ?
oleoyl-CoA + myelin proteolipid protein
-
 Rattus norvegicus CoA + myelin proteolipid O-oleoylprotein
-
 ?
palmitoyl-CoA + myelin proteolipid protein
-
 Rattus norvegicus CoA + myelin proteolipid O-palmitoylprotein
-
 ?
stearoyl-CoA + myelin proteolipid protein
-
 Rattus norvegicus CoA + myelin proteolipid O-stearoylprotein
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Rattus norvegicus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
10 50 45% of maximal acticity at 10°C, 75% of maximal activity at 50°C Rattus norvegicus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
75
-
 5 min, complete loss of activity Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
 Rattus norvegicus

pH Range

pH Minimum pH Maximum Comment Organism
6 8 25% of maximal activity at pH 6, 90% of maximal activity at pH 8 Rattus norvegicus