Any feedback?
Literature summary for 2.2.1.9 extracted from
- Structural views along the Mycobacterium tuberculosis MenD reaction pathway illuminate key aspects of thiamin diphosphate-dependent enzyme mechanisms (2016), Structure, 24, 1167-1177 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Mycobacterium smegmatis strain mc24517 | Mycobacterium tuberculosis |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| apoenzyme and in complex with thiamine diphosphate and Mg2+, sitting drop vapor diffusion method | Mycobacterium tuberculosis |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required for activity, 1 mM used in assay conditions | Mycobacterium tuberculosis |  |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| isochorismate + 2-oxoglutarate | Mycobacterium tuberculosis | - |
5-enolpyruvoyl-6-hydroxy-2-succinyl-cyclohex-3-ene-1-carboxylate + CO2 | - |
? | |
| isochorismate + 2-oxoglutarate | Mycobacterium tuberculosis H37Rv | - |
5-enolpyruvoyl-6-hydroxy-2-succinyl-cyclohex-3-ene-1-carboxylate + CO2 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mycobacterium tuberculosis | P9WK11 | - |
- |
| Mycobacterium tuberculosis H37Rv | P9WK11 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| IMAC column chromatography and gel filtration | Mycobacterium tuberculosis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| isochorismate + 2-oxoglutarate | - |
Mycobacterium tuberculosis | 5-enolpyruvoyl-6-hydroxy-2-succinyl-cyclohex-3-ene-1-carboxylate + CO2 | - |
? | |
| isochorismate + 2-oxoglutarate | - |
Mycobacterium tuberculosis H37Rv | 5-enolpyruvoyl-6-hydroxy-2-succinyl-cyclohex-3-ene-1-carboxylate + CO2 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | x-ray crystallography | Mycobacterium tuberculosis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexadiene-1-carboxylate synthase | - |
Mycobacterium tuberculosis |
| MenD | - |
Mycobacterium tuberculosis |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| thiamine diphosphate | dependent on | Mycobacterium tuberculosis | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
