Literature summary for 2.2.1.7 extracted from

Handa, S.; Ramamoorthya, D.; Spradlinga, T.J.; Guidaa, W.C.; Adamsb, J.H.; Bendinskasc, K.G.; Merkler, D.J.
Production of recombinant 1-deoxy-D-xylulose-5-phosphate synthase from Plasmodium vivax in Escherichia coli (2013), FEBS Open Bio, 3, 124-129.

Search for more literature for 2.2.1.7

Application

Application	Comment	Organism
medicine	development of 1-deoxy-D-xylulose-5-phosphate synthase inhibitors to treat Plasmodium vivax malaria	Plasmodium vivax

Cloned(Commentary)

Cloned (Comment)	Organism
use of a step wise deletion approach for the successful production of a catalytically active and soluble form of Plasmodium vivax 1-deoxy-D-xylulose-5-phosphate synthase in Escherichia coli. Signal and transit peptide removed soluble and functionally active enzyme is expressed in Escherichia coli	Plasmodium vivax

Inhibitors

Inhibitors	Comment	Organism	Structure
3-Fluoropyruvate	competitive with respect to pyruvate	Plasmodium vivax
EDTA	completely active after dialysis against EDTA. Incubation of the enzyme with saturated ammonium sulfate and 5.0mM EDTA at pH 3.5 leads to the complete loss of DXScc activity, with only a 25% recovery of activity upon the addition of Mg(II)	Plasmodium vivax

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.019	-	D-glyceraldehyde 3-phosphate	pH and temperature not specified in the publication	Plasmodium vivax
0.87	-	pyruvate	pH and temperature not specified in the publication	Plasmodium vivax

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	molar ratio of Mg2+ to enzyme is 0.97. The results suggest that Mg(II) may serve both a structural and catalytic role in the catalytic core and that Mg(II) is bound to the catalytic core with a higher affinity than thiamine diphosphate	Plasmodium vivax

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
103822	-	2 * 103822, MALDI-MS analysis	Plasmodium vivax
103921	-	2 * 103921, calculated from sequence	Plasmodium vivax
115000	-	2 * 115000, SDS-PAGE	Plasmodium vivax
260000	-	gel filtratiion	Plasmodium vivax

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
pyruvate + D-glyceraldehyde 3-phosphate	Plasmodium vivax	-	1-deoxy-D-xylulose 5-phosphate + CO2	-	?

Organism

Organism	UniProt	Comment	Textmining
Plasmodium vivax	A5K817	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Plasmodium vivax

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
1.2	-	pH and temperature not specified in the publication	Plasmodium vivax

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
pyruvate + D-glyceraldehyde 3-phosphate	-	Plasmodium vivax	1-deoxy-D-xylulose 5-phosphate + CO2	-	?

Subunits

Subunits	Comment	Organism
homodimer	2 * 115000, SDS-PAGE	Plasmodium vivax
homodimer	2 * 103822, MALDI-MS analysis	Plasmodium vivax
homodimer	2 * 103921, calculated from sequence	Plasmodium vivax

Synonyms

Synonyms	Comment	Organism
DXS	-	Plasmodium vivax

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
9.5	-	pyruvate	pH and temperature not specified in the publication	Plasmodium vivax
11	-	D-glyceraldehyde 3-phosphate	pH and temperature not specified in the publication	Plasmodium vivax

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7	7.5	-	Plasmodium vivax

Cofactor

Cofactor	Comment	Organism	Structure
thiamine diphosphate	-	Plasmodium vivax

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.2	-	3-Fluoropyruvate	pH and temperature not specified in the publication	Plasmodium vivax

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
11	-	pyruvate	pH and temperature not specified in the publication	Plasmodium vivax
570	-	D-glyceraldehyde 3-phosphate	pH and temperature not specified in the publication	Plasmodium vivax

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Release 2023.1 (January 2023)