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Literature summary for 2.2.1.6 extracted from

  • Krause, F.S.; Blombach, B.; Eikmanns, B.J.
    Metabolic engineering of Corynebacterium glutamicum for 2-ketoisovalerate production (2010), Appl. Environ. Microbiol., 76, 8053-8061.
    View publication on PubMedView publication on EuropePMC

Application

Application Comment Organism
synthesis engineering of the wild type of Corynebacterium glutamicum for the growth-decoupled production of 2-ketoisovalerate from glucose by deletion of the aceE gene encoding the E1p subunit of the pyruvate dehydrogenase complex, deletion of the transaminase B gene ilvE, and additional overexpression of the ilvBNCD genes, encoding the L-valine biosynthetic enzymes acetohydroxyacid synthase (AHAS), acetohydroxyacid isomeroreductase, and dihydroxyacid dehydratase. 2-Ketoisovalerate production is further improved by deletion of the pyruvate:quinone oxidoreductase gene pqo. In fed-batch fermentations at high cell densities, the newly constructed strains produce up to 188 mM (21.8 g/liter) 2-ketoisovalerate and showd a product yield of about 0.47 mol per mol (0.3 g/g) of glucose and a volumetric productivity of about 4.6 mM (0.53 g/liter) 2-ketoisovalerate per h in the overall production phase Corynebacterium glutamicum

Inhibitors

Inhibitors Comment Organism Structure
2-oxoisovalerate competitive Corynebacterium glutamicum

Organism

Organism UniProt Comment Textmining
Corynebacterium glutamicum P42463 P42463, i.e. acetolactate synthase large subunit ilvB
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Corynebacterium glutamicum DSM 20300 P42463 P42463, i.e. acetolactate synthase large subunit ilvB
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Synonyms

Synonyms Comment Organism
ilvB
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Corynebacterium glutamicum