Cloned (Comment) | Organism |
---|---|
gene AHAS, expression of the His-tagged enzyme in Escherichia coli strain BL21(DE3), the enzyme contains additional 34 residues, MGSSHHHHHHSSGLVPRGSHMASMTGGQQMGRGS, at the N-terminus | Bacillus anthracis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cadre | an imidazolinone herbicide | Bacillus anthracis | |
Londax | a sulfonylurea herbicide | Bacillus anthracis | |
additional information | AHAS from Bacillus anthracis shows strong resistance to three classes of herbicides, the sulfonylurea Londax, the imidazolinone Cadre, and the triazolopyrimidine TP | Bacillus anthracis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.28 | - |
thiamine diphosphate | - |
Bacillus anthracis | |
4.8 | - |
pyruvate | pH 7.4, 37°C, recombinant enzyme | Bacillus anthracis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | K0.5 is 1.160 mM | Bacillus anthracis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
65000 | - |
x * 65000, recombinant enzyme, SDS-PAGE | Bacillus anthracis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 pyruvate | Bacillus anthracis | acetohydroxyacid synthase is the enzyme that catalyses the first step in the common pathway of the biosynthesis of the branched chain amino acids, valine, leucine and isoleucine | 2-acetolactate + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus anthracis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
His-tagged recombinant enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography to 96% purity | Bacillus anthracis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.5 | - |
calculated per subunit, purified recombinant His-tagged enzyme | Bacillus anthracis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 pyruvate | - |
Bacillus anthracis | 2-acetolactate + CO2 | - |
? | |
2 pyruvate | acetohydroxyacid synthase is the enzyme that catalyses the first step in the common pathway of the biosynthesis of the branched chain amino acids, valine, leucine and isoleucine | Bacillus anthracis | 2-acetolactate + CO2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 65000, recombinant enzyme, SDS-PAGE | Bacillus anthracis |
Synonyms | Comment | Organism |
---|---|---|
acetohydroxyacid synthase | - |
Bacillus anthracis |
AHAS | - |
Bacillus anthracis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Bacillus anthracis |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
10 | 50 | 30°C: about 50% of maximal activity, 50°C: about 65% of maximal activity, temperature profile | Bacillus anthracis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Bacillus anthracis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 10 | pH 6.5: about 40% of maximal activity, pH 10.0: about 65% of maximal activity, pH profile | Bacillus anthracis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Bacillus anthracis | |
thiamine diphosphate | - |
Bacillus anthracis |