Literature summary for 2.2.1.6 extracted from

Choi, K.J.; Noh, K.M.; Kim, D.E.; Ha, B.H.; Kim, E.E.; Yoon, M.Y.
Identification of the catalytic subunit of acetohydroxyacid synthase in Haemophilus influenzae and its potent inhibitors (2007), Arch. Biochem. Biophys., 466, 24-30.

Search for more literature for 2.2.1.6

Cloned(Commentary)

Cloned (Comment)	Organism
His-tagged AHAS catalytic subunit , overexpression in Escherichia coli strain BL21(DE3)	Haemophilus influenzae

Inhibitors

Inhibitors	Comment	Organism	Structure
AVS-2087	-	Haemophilus influenzae
KHG20612	-	Haemophilus influenzae
KSW30191	-	Haemophilus influenzae
additional information	inhibitor library screening	Haemophilus influenzae
sulfometuron methyl	-	Haemophilus influenzae

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
9.2	-	pyruvate	pH 7.5, 37°C, recombinant enzyme	Haemophilus influenzae

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	-	Haemophilus influenzae

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
63700	-	x * 63700, recombinant His-tagged catalytic subunit, SDS-PAGE	Haemophilus influenzae

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
2 pyruvate	Haemophilus influenzae	AHAS catalyzes the first common step in the biosynthetic pathway of the branched-amino acids leucine, isoleucine, and valine	2-acetolactate + CO2	-	?

Organism

Organism	UniProt	Comment	Textmining
Haemophilus influenzae	P45261	large subunit	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant His-tagged AHAS catalytic subunit from Escherichia coli strain BL21(DE3) to homogeneity by nickel affinity and anion exchange chromatography, and gel filtration	Haemophilus influenzae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
1.5	-	purified recombinant enzyme	Haemophilus influenzae

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2 pyruvate	AHAS catalyzes the first common step in the biosynthetic pathway of the branched-amino acids leucine, isoleucine, and valine	Haemophilus influenzae	2-acetolactate + CO2	-	?
2 pyruvate	condensation reaction	Haemophilus influenzae	2-acetolactate + CO2	-	?

Subunits

Subunits	Comment	Organism
?	x * 63700, recombinant His-tagged catalytic subunit, SDS-PAGE	Haemophilus influenzae

Synonyms

Synonyms	Comment	Organism
acetohydroxyacid synthase	-	Haemophilus influenzae
AHAS	-	Haemophilus influenzae

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	assay at	Haemophilus influenzae

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.5	-	assay at	Haemophilus influenzae

Cofactor

Cofactor	Comment	Organism	Structure
FAD	dependent on	Haemophilus influenzae
thiamine diphosphate	dependent on	Haemophilus influenzae

IC50 Value

IC50 Value	IC50 Value Maximum	Comment	Organism	Inhibitor	Structure
0.00053	-	pH 7.5, 37°C, recombinant enzyme	Haemophilus influenzae	AVS-2087
0.00142	-	pH 7.5, 37°C, recombinant enzyme	Haemophilus influenzae	KSW30191
0.00491	-	pH 7.5, 37°C, recombinant enzyme	Haemophilus influenzae	KHG20612
0.2763	-	pH 7.5, 37°C, recombinant enzyme	Haemophilus influenzae	sulfometuron methyl

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Release 2023.1 (January 2023)