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Literature summary for 2.2.1.6 extracted from

  • Choi, K.; Noh, K.M.; Choi, J.; Park, J.; Won, H.; Kim, J.; Kim, J.; Yoon, M.
    Sulfonylurea is a non-competitive inhibitor of acetohydroxyacid synthase from Mycobacterium tuberculosis (2006), Bull. Korean Chem. Soc., 27, 1697-1700.
No PubMed abstract available

Cloned(Commentary)

Cloned (Comment) Organism
expression of the His-tagged catalytic subunit Mycobacterium tuberculosis

Inhibitors

Inhibitors Comment Organism Structure
chlorimuron ethyl IC50: 0.009 mM, over 80% inhibition at 0.04 mM Mycobacterium tuberculosis
metsulfuron methyl IC50: 0.006 mM, over 80% inhibition at 0.04 mM Mycobacterium tuberculosis
additional information mechanism and potency of enzyme inhibition by sulfonylurea herbicides, overview Mycobacterium tuberculosis
primisulfuron methyl IC50: 0.0042 mM, over 80% inhibition at 0.04 mM Mycobacterium tuberculosis
pyrazosulfuron ethyl IC50: 87 nM, over 80% inhibition at 0.04 mM Mycobacterium tuberculosis
sulfometuron methyl IC50: 0.0048 mM, over 80% inhibition at 0.04 mM Mycobacterium tuberculosis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.76
-
pyruvate catalytic subunit Mycobacterium tuberculosis

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ a divalent metal ion is required Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
pyruvate Mycobacterium tuberculosis first committed step in the biosynthesis of valine and leucine 2-acetolactate + CO2
-
?
pyruvate Mycobacterium tuberculosis H37Rv first committed step in the biosynthesis of valine and leucine 2-acetolactate + CO2
-
?
pyruvate + 2-oxobutyrate Mycobacterium tuberculosis first committed step in the biosynthesis of isoleucine 2-aceto-2-hydroxybutyrate + CO2
-
?
pyruvate + 2-oxobutyrate Mycobacterium tuberculosis H37Rv first committed step in the biosynthesis of isoleucine 2-aceto-2-hydroxybutyrate + CO2
-
?

Organism

Organism UniProt Comment Textmining
Mycobacterium tuberculosis
-
-
-
Mycobacterium tuberculosis H37Rv
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged catalytic subunit to homogeneity Mycobacterium tuberculosis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
pyruvate
-
Mycobacterium tuberculosis 2-acetolactate + CO2
-
?
pyruvate first committed step in the biosynthesis of valine and leucine Mycobacterium tuberculosis 2-acetolactate + CO2
-
?
pyruvate
-
Mycobacterium tuberculosis H37Rv 2-acetolactate + CO2
-
?
pyruvate first committed step in the biosynthesis of valine and leucine Mycobacterium tuberculosis H37Rv 2-acetolactate + CO2
-
?
pyruvate + 2-oxobutyrate
-
Mycobacterium tuberculosis 2-aceto-2-hydroxybutyrate + CO2
-
?
pyruvate + 2-oxobutyrate first committed step in the biosynthesis of isoleucine Mycobacterium tuberculosis 2-aceto-2-hydroxybutyrate + CO2
-
?
pyruvate + 2-oxobutyrate
-
Mycobacterium tuberculosis H37Rv 2-aceto-2-hydroxybutyrate + CO2
-
?
pyruvate + 2-oxobutyrate first committed step in the biosynthesis of isoleucine Mycobacterium tuberculosis H37Rv 2-aceto-2-hydroxybutyrate + CO2
-
?

Subunits

Subunits Comment Organism
More the enzyme consists of two diffrent types of subunits, a catalytic one and a regulatory one Mycobacterium tuberculosis

Synonyms

Synonyms Comment Organism
acetohydroxyacid synthase
-
Mycobacterium tuberculosis
AHAS
-
Mycobacterium tuberculosis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Mycobacterium tuberculosis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.19
-
pyruvate catalytic subunit Mycobacterium tuberculosis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Mycobacterium tuberculosis

Cofactor

Cofactor Comment Organism Structure
FAD
-
Mycobacterium tuberculosis
thiamine diphosphate
-
Mycobacterium tuberculosis

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
additional information
-
additional information inhibition kinetics of sulfonylurea herbicides Mycobacterium tuberculosis

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.000087
-
IC50: 87 nM, over 80% inhibition at 0.04 mM Mycobacterium tuberculosis pyrazosulfuron ethyl
0.0042
-
IC50: 0.0042 mM, over 80% inhibition at 0.04 mM Mycobacterium tuberculosis primisulfuron methyl
0.0048
-
IC50: 0.0048 mM, over 80% inhibition at 0.04 mM Mycobacterium tuberculosis sulfometuron methyl
0.006
-
IC50: 0.006 mM, over 80% inhibition at 0.04 mM Mycobacterium tuberculosis metsulfuron methyl
0.009
-
IC50: 0.009 mM, over 80% inhibition at 0.04 mM Mycobacterium tuberculosis chlorimuron ethyl