Cloned (Comment) | Organism |
---|---|
expression of the His-tagged catalytic subunit | Mycobacterium tuberculosis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
chlorimuron ethyl | IC50: 0.009 mM, over 80% inhibition at 0.04 mM | Mycobacterium tuberculosis | |
metsulfuron methyl | IC50: 0.006 mM, over 80% inhibition at 0.04 mM | Mycobacterium tuberculosis | |
additional information | mechanism and potency of enzyme inhibition by sulfonylurea herbicides, overview | Mycobacterium tuberculosis | |
primisulfuron methyl | IC50: 0.0042 mM, over 80% inhibition at 0.04 mM | Mycobacterium tuberculosis | |
pyrazosulfuron ethyl | IC50: 87 nM, over 80% inhibition at 0.04 mM | Mycobacterium tuberculosis | |
sulfometuron methyl | IC50: 0.0048 mM, over 80% inhibition at 0.04 mM | Mycobacterium tuberculosis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.76 | - |
pyruvate | catalytic subunit | Mycobacterium tuberculosis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | a divalent metal ion is required | Mycobacterium tuberculosis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
pyruvate | Mycobacterium tuberculosis | first committed step in the biosynthesis of valine and leucine | 2-acetolactate + CO2 | - |
? | |
pyruvate | Mycobacterium tuberculosis H37Rv | first committed step in the biosynthesis of valine and leucine | 2-acetolactate + CO2 | - |
? | |
pyruvate + 2-oxobutyrate | Mycobacterium tuberculosis | first committed step in the biosynthesis of isoleucine | 2-aceto-2-hydroxybutyrate + CO2 | - |
? | |
pyruvate + 2-oxobutyrate | Mycobacterium tuberculosis H37Rv | first committed step in the biosynthesis of isoleucine | 2-aceto-2-hydroxybutyrate + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | - |
- |
- |
Mycobacterium tuberculosis H37Rv | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged catalytic subunit to homogeneity | Mycobacterium tuberculosis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
pyruvate | - |
Mycobacterium tuberculosis | 2-acetolactate + CO2 | - |
? | |
pyruvate | first committed step in the biosynthesis of valine and leucine | Mycobacterium tuberculosis | 2-acetolactate + CO2 | - |
? | |
pyruvate | - |
Mycobacterium tuberculosis H37Rv | 2-acetolactate + CO2 | - |
? | |
pyruvate | first committed step in the biosynthesis of valine and leucine | Mycobacterium tuberculosis H37Rv | 2-acetolactate + CO2 | - |
? | |
pyruvate + 2-oxobutyrate | - |
Mycobacterium tuberculosis | 2-aceto-2-hydroxybutyrate + CO2 | - |
? | |
pyruvate + 2-oxobutyrate | first committed step in the biosynthesis of isoleucine | Mycobacterium tuberculosis | 2-aceto-2-hydroxybutyrate + CO2 | - |
? | |
pyruvate + 2-oxobutyrate | - |
Mycobacterium tuberculosis H37Rv | 2-aceto-2-hydroxybutyrate + CO2 | - |
? | |
pyruvate + 2-oxobutyrate | first committed step in the biosynthesis of isoleucine | Mycobacterium tuberculosis H37Rv | 2-aceto-2-hydroxybutyrate + CO2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | the enzyme consists of two diffrent types of subunits, a catalytic one and a regulatory one | Mycobacterium tuberculosis |
Synonyms | Comment | Organism |
---|---|---|
acetohydroxyacid synthase | - |
Mycobacterium tuberculosis |
AHAS | - |
Mycobacterium tuberculosis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Mycobacterium tuberculosis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.19 | - |
pyruvate | catalytic subunit | Mycobacterium tuberculosis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Mycobacterium tuberculosis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Mycobacterium tuberculosis | |
thiamine diphosphate | - |
Mycobacterium tuberculosis |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | inhibition kinetics of sulfonylurea herbicides | Mycobacterium tuberculosis |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.000087 | - |
IC50: 87 nM, over 80% inhibition at 0.04 mM | Mycobacterium tuberculosis | pyrazosulfuron ethyl | |
0.0042 | - |
IC50: 0.0042 mM, over 80% inhibition at 0.04 mM | Mycobacterium tuberculosis | primisulfuron methyl | |
0.0048 | - |
IC50: 0.0048 mM, over 80% inhibition at 0.04 mM | Mycobacterium tuberculosis | sulfometuron methyl | |
0.006 | - |
IC50: 0.006 mM, over 80% inhibition at 0.04 mM | Mycobacterium tuberculosis | metsulfuron methyl | |
0.009 | - |
IC50: 0.009 mM, over 80% inhibition at 0.04 mM | Mycobacterium tuberculosis | chlorimuron ethyl |