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Literature summary for 2.2.1.6 extracted from

  • Oh, K.J.; Park, E.J.; Yoon, M.Y.; Han, T.R.; Choi, J.D.
    Roles of histidine residues in tobacco acetolactate synthase (2001), Biochem. Biophys. Res. Commun., 282, 1237-1243.
    View publication on PubMed
Search for more literature for 2.2.1.6

Protein Variants

Protein Variants Comment Organism
H351F 5fold increase in Km-value, weak resistance to Londax and Cadre, difference in secondary structure compared to wild-type Nicotiana tabacum
H351M 18fold increase in Km-value, strong resistance to Londax and Cadre, difference in secondary structure compared to wild-type Nicotiana tabacum
H351Q 60fold increase in Km-value, strong resistance to Londax and Cadre, difference in secondary structure compared to wild-type Nicotiana tabacum
H392M no significant effects Nicotiana tabacum
H487F no enzymic activity, no affinity for FAD Nicotiana tabacum
H487L no enzymic activity, no affinity for FAD Nicotiana tabacum

Inhibitors

Inhibitors Comment Organism Structure
2-(5-ethyl-3-methylpyridin-2-yl)-5-isopropyl-5-methyl-3,5-dihydro-4H-imidazol-4-one i.e. Cadre Nicotiana tabacum
N-(4,6-dimethylpyrimidin-2-yl)-5-methyl-6,7,8,8a-tetrahydro-5aH-cyclopenta[e][1,2,4]triazolo[1,5-a]pyrimidine-2-sulfonamide i.e. TP Nicotiana tabacum
N-[[(4-methoxy-1,3,5-triazin-2-yl)amino]carbonyl]-1-phenylmethanesulfonamide - methyl hydroperoxide ratio 1:1, i.e. Londax Nicotiana tabacum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
13.71
-
 pyruvate mutant H392M, 37°C, pH 7.5 Nicotiana tabacum
16.09
-
 pyruvate wild-type, 37°C, pH 7.5 Nicotiana tabacum
76.63
-
 pyruvate mutantH351F, 37°C, pH 7.5 Nicotiana tabacum
287.8
-
 pyruvate mutant H351M, 37°C, pH 7.5 Nicotiana tabacum
959.5
-
 pyruvate mutant H351Q, 37°C, pH 7.5 Nicotiana tabacum

Organism

Organism UniProt Comment Textmining
Nicotiana tabacum
-
 expression in Escherichia coli
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.03
-
 mutant H351Q, 37°C, pH 7.5 Nicotiana tabacum
0.04
-
 mutant H351M, 37°C, pH 7.5 Nicotiana tabacum
1.58
-
 mutantH351F, 37°C, pH 7.5 Nicotiana tabacum
3.23
-
 mutant H392M, 37°C, pH 7.5 Nicotiana tabacum
5.78
-
 wild-type, 37°C, pH 7.5 Nicotiana tabacum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
pyruvate
-
 Nicotiana tabacum 2-acetolactate + CO2
-
 ?

Cofactor

Cofactor Comment Organism Structure
FAD
-
 Nicotiana tabacum

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.00000045
-
 N-(4,6-dimethylpyrimidin-2-yl)-5-methyl-6,7,8,8a-tetrahydro-5aH-cyclopenta[e][1,2,4]triazolo[1,5-a]pyrimidine-2-sulfonamide mutantH351F, 37°C, pH 7.5 Nicotiana tabacum
0.00000311
-
 N-(4,6-dimethylpyrimidin-2-yl)-5-methyl-6,7,8,8a-tetrahydro-5aH-cyclopenta[e][1,2,4]triazolo[1,5-a]pyrimidine-2-sulfonamide wild-type, 37°C, pH 7.5 Nicotiana tabacum
0.00000365
-
 N-(4,6-dimethylpyrimidin-2-yl)-5-methyl-6,7,8,8a-tetrahydro-5aH-cyclopenta[e][1,2,4]triazolo[1,5-a]pyrimidine-2-sulfonamide mutant H392M, 37°C, pH 7.5 Nicotiana tabacum
0.00000368
-
 N-(4,6-dimethylpyrimidin-2-yl)-5-methyl-6,7,8,8a-tetrahydro-5aH-cyclopenta[e][1,2,4]triazolo[1,5-a]pyrimidine-2-sulfonamide mutant H351M, 37°C, pH 7.5 Nicotiana tabacum
0.0000093
-
 N-[[(4-methoxy-1,3,5-triazin-2-yl)amino]carbonyl]-1-phenylmethanesulfonamide - methyl hydroperoxide mutant H392M, 37°C, pH 7.5 Nicotiana tabacum
0.0000109
-
 N-[[(4-methoxy-1,3,5-triazin-2-yl)amino]carbonyl]-1-phenylmethanesulfonamide - methyl hydroperoxide wild-type, 37°C, pH 7.5 Nicotiana tabacum
0.00002304
-
 N-[[(4-methoxy-1,3,5-triazin-2-yl)amino]carbonyl]-1-phenylmethanesulfonamide - methyl hydroperoxide mutantH351F, 37°C, pH 7.5 Nicotiana tabacum
0.00004857
-
 N-(4,6-dimethylpyrimidin-2-yl)-5-methyl-6,7,8,8a-tetrahydro-5aH-cyclopenta[e][1,2,4]triazolo[1,5-a]pyrimidine-2-sulfonamide mutant H351Q, 37°C, pH 7.5 Nicotiana tabacum
0.00135
-
 2-(5-ethyl-3-methylpyridin-2-yl)-5-isopropyl-5-methyl-3,5-dihydro-4H-imidazol-4-one wild-type, 37°C, pH 7.5 Nicotiana tabacum
0.00205
-
 2-(5-ethyl-3-methylpyridin-2-yl)-5-isopropyl-5-methyl-3,5-dihydro-4H-imidazol-4-one mutant H392M, 37°C, pH 7.5 Nicotiana tabacum
0.01735
-
 2-(5-ethyl-3-methylpyridin-2-yl)-5-isopropyl-5-methyl-3,5-dihydro-4H-imidazol-4-one mutantH351F, 37°C, pH 7.5 Nicotiana tabacum