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Literature summary for 2.2.1.6 extracted from
- Expression in Escherichia coli, purification, and characterization of the tobacco sulfonylurea herbicide-resistant recombinant acetolactate synthase and its interaction with the triazolopyrimidine herbicides (1998), J. Biochem. Mol. Biol., 31, 287-295.
No PubMed abstract available
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| cloned into the bacterial expression vector pGEX-2T and used to transform Escherichia coli strain pGEX-2T | Nicotiana tabacum |
| GST-wALS, and sulfonylurea-resistant enzyme fused with glutathione S-transferase, GST-mALS | Nicotiana tabacum |
| wild-type recombinant enzyme fused with glutathione S-transferase | Nicotiana tabacum |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| pyruvate | Nicotiana tabacum | - |
? | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Nicotiana tabacum | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| pyruvate | - |
Nicotiana tabacum | 2-acetolactate + CO2 | - |
? | |
| pyruvate | - |
Nicotiana tabacum | ? | - |
? | |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | - |
Nicotiana tabacum | |
| thiamine diphosphate | - |
Nicotiana tabacum | |
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