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Literature summary for 2.2.1.5 extracted from
- Purification, general properties and two other catalytic activities of alpha-ketoglutarate:glyoxylate carboligase of Mycobacterium phlei (1971), Biochim. Biophys. Acta, 242, 637-647.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | 93% inhibition at 1 mM | Mycolicibacterium phlei |  |
| iodoacetic acid | 83% inhibition at 3.3 mM | Mycolicibacterium phlei | |
| p-chloromercuriphenylsulfonic acid | complete inhibition at 1 mM | Mycolicibacterium phlei | |
| Zn2+ | 97% inhibition at 1 mM | Mycolicibacterium phlei | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2 | - |
2-oxoglutarate | - |
Mycolicibacterium phlei | |
| 3.2 | - |
glyoxylate | - |
Mycolicibacterium phlei | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | 31% activation at 1 mM | Mycolicibacterium phlei | |
| Mn2+ | 175% activation at 1 mM | Mycolicibacterium phlei | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mycolicibacterium phlei | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Mycolicibacterium phlei |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| 2-oxoglutarate + glyoxylate = 2-hydroxy-3-oxoadipate + CO2 | mechanism | Mycolicibacterium phlei |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 2.21 | - |
- |
Mycolicibacterium phlei |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-oxoglutarate | - |
Mycolicibacterium phlei | ? + CO2 | - |
? | |
| 2-oxoglutarate + acetaldehyde | - |
Mycolicibacterium phlei | 5-hydroxy-4-oxohexanoate + CO2 | - |
? | |
| 2-oxoglutarate + glyoxylate | - |
Mycolicibacterium phlei | 2-hydroxy-3-oxohexandioate + CO2 | further spontaneous decarboxylation in the presence of acid | ? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
enzyme assay at | Mycolicibacterium phlei |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.3 | - |
- |
Mycolicibacterium phlei |
| 6.5 | - |
enzyme assay at | Mycolicibacterium phlei |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| thiamine diphosphate | - |
Mycolicibacterium phlei | |
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