BRENDA - Enzyme Database
show all sequences of 2.2.1.3

Dihydroxyacetone synthase: a special transketolase for formaldehyde fixation from the methylotrophic yeast Candida boidinii CBS 5777

Waites, M.J.; Quayle, J.R.; J. Gen. Microbiol. 129, 935-944 (1983)
No PubMed abstract available

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
Co2+
-
Candida boidinii
GSH
-
Candida boidinii
Ni2+
-
Candida boidinii
ribose
-
Candida boidinii
sulfate
-
Candida boidinii
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Mg2+
requirement
Candida boidinii
Mn2+
activation, replaces Mg2+ to some extent
Candida boidinii
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
62500
-
alpha2, 2 * 62500, non-equilibrium pH-gradient electrophoresis followed by SDS-PAGE
Candida boidinii
65500
-
alpha2, 2 * 65500, SDS-PAGE
Candida boidinii
105000
110000
gel filtration
Candida boidinii
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
D-xylulose 5-phosphate + formaldehyde
Candida boidinii
-
glyceraldehyde 3-phosphate + dihydroxyacetone
i.e. 1,3-dihydroxy-2-propanone
Candida boidinii
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Candida boidinii
-
methanol grown
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.766
-
-
Candida boidinii
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D-xylulose 5-phosphate + butyraldehyde
-
486051
Candida boidinii
glyceraldehyde 3-phosphate + 1,3-dihydroxyhexane-2-one
-
-
-
?
D-xylulose 5-phosphate + D-erythrose
-
486051
Candida boidinii
glyceraldehyde 3-phosphate + D-fructose
-
-
-
?
D-xylulose 5-phosphate + D-glucose
-
486051
Candida boidinii
glyceraldehyde 3-phosphate + D-glycero-D-ido-oct-2-ulose
-
-
-
?
D-xylulose 5-phosphate + D-ribose
-
486051
Candida boidinii
glyceraldehyde 3-phosphate + sedoheptulose
-
-
-
?
D-xylulose 5-phosphate + formaldehyde
-
486051
Candida boidinii
glyceraldehyde 3-phosphate + dihydroxyacetone
i.e. 1,3-dihydroxy-2-propanone
486051
Candida boidinii
-
D-xylulose 5-phosphate + formaldehyde
best substrate
486051
Candida boidinii
glyceraldehyde 3-phosphate + dihydroxyacetone
i.e. 1,3-dihydroxy-2-propanone
486051
Candida boidinii
-
D-xylulose 5-phosphate + formaldehyde
D-xylulose 5-phosphate cannot be replaced by D-ribulose 5-phosphate, D-glucose 6-phosphate, or D-ribose 5-phosphate
486051
Candida boidinii
glyceraldehyde 3-phosphate + dihydroxyacetone
i.e. 1,3-dihydroxy-2-propanone
486051
Candida boidinii
-
D-xylulose 5-phosphate + formaldehyde
no acceptors are glyceraldehyde, glyceraldehyde 3-phosphate, erythrose 4-phosphate, ribose 5-phosphate, glucose 6-phosphate, D-ribose, glutaraldehyde, dihydroxyacetone
486051
Candida boidinii
glyceraldehyde 3-phosphate + dihydroxyacetone
i.e. 1,3-dihydroxy-2-propanone
486051
Candida boidinii
-
D-xylulose 5-phosphate + glycolaldehyde
-
486051
Candida boidinii
glyceraldehyde 3-phosphate + 1,3,5-trihydroxypentane-2-one
-
-
-
?
D-xylulose 5-phosphate + heptaldehyde
-
486051
Candida boidinii
glyceraldehyde 3-phosphate + 1,3-dihydroxynonane-2-one
-
-
-
?
D-xylulose 5-phosphate + propionaldehyde
-
486051
Candida boidinii
glyceraldehyde 3-phosphate + 1,3-dihydroxypentane-2-one
-
-
-
?
fructose 6-phosphate + formaldehyde
fructose 6-phosphate can replace D-xylulose 5-phosphate with 10% efficiency as glycolaldehyde donor
486051
Candida boidinii
dihydroxyacetone + erythrose 4-phosphate
-
-
-
?
hydroxypyruvate + formaldehyde
hydroxypyruvate can replace D-xylulose 5-phosphate with 41.4%, hydroxypyruvate can replace D-xylulose 5-phosphate with 56%
486051
Candida boidinii
CO2 + dihydroxyacetone
-
-
-
?
sedoheptulose 7-phosphate + formaldehyde
sedoheptulose 7-phosphate can replace D-xylulose 5-phosphate with 10% efficiency as glycolaldehyde donor
486051
Candida boidinii
dihydroxyacetone + ribose
-
-
-
-
Subunits
Subunits
Commentary
Organism
dimer
alpha2, 2 * 62500, non-equilibrium pH-gradient electrophoresis followed by SDS-PAGE; alpha2, 2 * 65500, SDS-PAGE
Candida boidinii
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
35
-
15 min in stabilizing buffer; 5 min without stabilizing agents
Candida boidinii
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Co2+
-
Candida boidinii
GSH
-
Candida boidinii
Ni2+
-
Candida boidinii
ribose
-
Candida boidinii
sulfate
-
Candida boidinii
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Mg2+
requirement
Candida boidinii
Mn2+
activation, replaces Mg2+ to some extent
Candida boidinii
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
62500
-
alpha2, 2 * 62500, non-equilibrium pH-gradient electrophoresis followed by SDS-PAGE
Candida boidinii
65500
-
alpha2, 2 * 65500, SDS-PAGE
Candida boidinii
105000
110000
gel filtration
Candida boidinii
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
D-xylulose 5-phosphate + formaldehyde
Candida boidinii
-
glyceraldehyde 3-phosphate + dihydroxyacetone
i.e. 1,3-dihydroxy-2-propanone
Candida boidinii
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.766
-
-
Candida boidinii
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D-xylulose 5-phosphate + butyraldehyde
-
486051
Candida boidinii
glyceraldehyde 3-phosphate + 1,3-dihydroxyhexane-2-one
-
-
-
?
D-xylulose 5-phosphate + D-erythrose
-
486051
Candida boidinii
glyceraldehyde 3-phosphate + D-fructose
-
-
-
?
D-xylulose 5-phosphate + D-glucose
-
486051
Candida boidinii
glyceraldehyde 3-phosphate + D-glycero-D-ido-oct-2-ulose
-
-
-
?
D-xylulose 5-phosphate + D-ribose
-
486051
Candida boidinii
glyceraldehyde 3-phosphate + sedoheptulose
-
-
-
?
D-xylulose 5-phosphate + formaldehyde
-
486051
Candida boidinii
glyceraldehyde 3-phosphate + dihydroxyacetone
i.e. 1,3-dihydroxy-2-propanone
486051
Candida boidinii
-
D-xylulose 5-phosphate + formaldehyde
best substrate
486051
Candida boidinii
glyceraldehyde 3-phosphate + dihydroxyacetone
i.e. 1,3-dihydroxy-2-propanone
486051
Candida boidinii
-
D-xylulose 5-phosphate + formaldehyde
D-xylulose 5-phosphate cannot be replaced by D-ribulose 5-phosphate, D-glucose 6-phosphate, or D-ribose 5-phosphate
486051
Candida boidinii
glyceraldehyde 3-phosphate + dihydroxyacetone
i.e. 1,3-dihydroxy-2-propanone
486051
Candida boidinii
-
D-xylulose 5-phosphate + formaldehyde
no acceptors are glyceraldehyde, glyceraldehyde 3-phosphate, erythrose 4-phosphate, ribose 5-phosphate, glucose 6-phosphate, D-ribose, glutaraldehyde, dihydroxyacetone
486051
Candida boidinii
glyceraldehyde 3-phosphate + dihydroxyacetone
i.e. 1,3-dihydroxy-2-propanone
486051
Candida boidinii
-
D-xylulose 5-phosphate + glycolaldehyde
-
486051
Candida boidinii
glyceraldehyde 3-phosphate + 1,3,5-trihydroxypentane-2-one
-
-
-
?
D-xylulose 5-phosphate + heptaldehyde
-
486051
Candida boidinii
glyceraldehyde 3-phosphate + 1,3-dihydroxynonane-2-one
-
-
-
?
D-xylulose 5-phosphate + propionaldehyde
-
486051
Candida boidinii
glyceraldehyde 3-phosphate + 1,3-dihydroxypentane-2-one
-
-
-
?
fructose 6-phosphate + formaldehyde
fructose 6-phosphate can replace D-xylulose 5-phosphate with 10% efficiency as glycolaldehyde donor
486051
Candida boidinii
dihydroxyacetone + erythrose 4-phosphate
-
-
-
?
hydroxypyruvate + formaldehyde
hydroxypyruvate can replace D-xylulose 5-phosphate with 41.4%, hydroxypyruvate can replace D-xylulose 5-phosphate with 56%
486051
Candida boidinii
CO2 + dihydroxyacetone
-
-
-
?
sedoheptulose 7-phosphate + formaldehyde
sedoheptulose 7-phosphate can replace D-xylulose 5-phosphate with 10% efficiency as glycolaldehyde donor
486051
Candida boidinii
dihydroxyacetone + ribose
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
alpha2, 2 * 62500, non-equilibrium pH-gradient electrophoresis followed by SDS-PAGE; alpha2, 2 * 65500, SDS-PAGE
Candida boidinii
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
35
-
15 min in stabilizing buffer; 5 min without stabilizing agents
Candida boidinii
Other publictions for EC 2.2.1.3
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
718524
Xiao
-
Overexpressions of dihydroxyac ...
Candida boidinii
Acta Physiol. Plant.
34
1975-1985
2012
-
-
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1
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-
-
-
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1
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1
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-
-
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-
688869
Seo
Cloning, characterization and ...
Mycobacterium sp., Mycobacterium sp. DSM 3803 / JC1
Microbiology
153
4174-4182
2007
-
-
1
-
-
-
-
-
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1
2
-
2
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2
1
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1
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1
2
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-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
486055
Stewart
Alcohol oxidase and dihydroxya ...
Candida boidinii
J. Cell Sci.
114
2863-2868
2001
-
-
-
-
-
-
-
-
1
-
-
1
-
1
-
-
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1
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1
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1
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-
-
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-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
486056
Salomons
Overproduction of Pex5p stimul ...
Ogataea angusta
J. Biol. Chem.
275
12603-12611
2000
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
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-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
486054
Sakai
Regulation and physiological r ...
Candida boidinii
J. Bacteriol.
180
5885-5890
1998
-
-
1
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
486057
Ro
Dihydroxyacetone synthase from ...
Acinetobacter sp.
J. Bacteriol.
179
6041-6047
1997
-
-
-
-
-
-
-
2
-
1
2
-
-
1
-
-
-
-
-
-
1
-
9
1
1
-
1
-
-
1
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
2
-
1
2
-
-
-
-
-
-
-
1
-
9
1
1
-
1
-
-
1
-
-
-
-
-
-
-
-
486053
Yanase
-
Enzymic preparation of [1,3-13 ...
Candida boidinii, Ogataea angusta
Appl. Microbiol. Biotechnol.
43
228-234
1995
-
-
-
-
-
-
-
-
-
-
-
2
-
2
-
-
-
-
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-
1
-
2
-
-
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-
-
-
-
-
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-
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-
-
2
-
-
-
-
-
-
1
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
486048
Bystrykh
Dihydroxyacetone synthase from ...
Candida boidinii, Candida boidinii KD1, Ogataea angusta, Ogataea angusta DL-1
Methods Enzymol.
188
435-445
1990
-
-
-
-
-
4
9
-
2
3
2
4
-
10
-
-
2
-
-
-
1
4
19
1
-
-
-
-
2
-
-
1
-
-
-
-
-
-
1
-
-
4
-
9
-
-
2
3
2
4
-
-
-
2
-
-
1
4
19
1
-
-
-
-
2
-
-
-
-
-
-
-
-
-
486052
Goodman
Dihydroxyacetone synthase is a ...
Candida boidinii
J. Biol. Chem.
260
7108-7113
1985
-
-
-
-
-
-
-
-
1
-
2
1
-
1
-
1
1
-
-
-
1
-
2
1
-
-
-
-
-
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-
-
-
-
-
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-
1
-
2
1
-
-
1
1
-
-
1
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
486051
Waites
-
Dihydroxyacetone synthase: a s ...
Candida boidinii
J. Gen. Microbiol.
129
935-944
1983
-
-
-
-
-
-
5
-
-
2
3
1
-
1
-
-
-
-
-
-
1
-
14
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
5
-
-
-
2
3
1
-
-
-
-
-
-
1
-
14
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
486047
Kato
Purification and properties of ...
Candida boidinii
Biochim. Biophys. Acta
715
143-150
1982
-
-
-
-
-
2
10
4
-
1
2
1
-
1
-
-
1
-
-
-
1
2
4
1
1
1
2
-
1
1
-
1
-
-
-
-
-
-
1
-
-
2
-
10
-
4
-
1
2
1
-
-
-
1
-
-
1
2
4
1
1
1
2
-
1
1
-
-
-
-
-
-
-
-
486046
Waites
The interrelation between tran ...
Candida boidinii
J. Gen. Microbiol.
124
309-316
1981
-
-
-
-
-
2
-
-
-
-
-
1
-
1
-
-
1
1
-
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1
2
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
1
2
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
486050
Bystrykh
-
Purification and properties of ...
Candida boidinii, Candida boidinii KD1
FEBS Lett.
132
324-328
1981
-
-
-
-
-
2
4
-
-
2
2
2
-
4
-
-
1
-
-
-
1
-
10
1
-
-
-
-
2
-
-
1
-
-
-
-
-
-
1
-
-
2
-
4
-
-
-
2
2
2
-
-
-
1
-
-
1
-
10
1
-
-
-
-
2
-
-
-
-
-
-
-
-
-
486049
O'Connor
-
Pentose phosphate-dependent fi ...
Candida boidinii, Ogataea angusta, Ogataea angusta DL-1
J. Gen. Microbiol.
120
219-225
1980
-
-
-
-
-
2
-
-
-
-
-
3
-
7
-
-
2
-
-
-
1
1
6
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
3
-
-
-
2
-
-
1
1
6
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-