General Stability | Organism |
---|---|
Ca2+ does not stabilize | [Candida] boidinii |
Mg2+ or NH4+ stabilizes for at least 80 h | [Candida] boidinii |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | - |
[Candida] boidinii | |
formaldehyde | above 1 mM, xylulose 5-phosphate protects | [Candida] boidinii | |
GSH | - |
[Candida] boidinii | |
NEM | irreversible, GSH partially protects | [Candida] boidinii |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | 5 mM, essential for effective binding of thiamine diphosphate to enzyme molecule | [Candida] boidinii | |
additional information | no activation by Ca2+ | [Candida] boidinii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
76000 | - |
alpha2, 2 * 76000, SDS-PAGE | [Candida] boidinii |
145000 | - |
gel filtration | [Candida] boidinii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-xylulose 5-phosphate + formaldehyde | [Candida] boidinii | - |
glyceraldehyde 3-phosphate + dihydroxyacetone | i.e. 1,3-dihydroxy-2-propanone | ir | |
D-xylulose 5-phosphate + formaldehyde | [Candida] boidinii KD1 | - |
glyceraldehyde 3-phosphate + dihydroxyacetone | i.e. 1,3-dihydroxy-2-propanone | ir |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
[Candida] boidinii | - |
- |
- |
[Candida] boidinii | - |
methanol grown | - |
[Candida] boidinii KD1 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
[Candida] boidinii |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
4 | - |
- |
[Candida] boidinii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-xylulose 5-phosphate + formaldehyde | - |
[Candida] boidinii | glyceraldehyde 3-phosphate + dihydroxyacetone | i.e. 1,3-dihydroxy-2-propanone | ir | |
D-xylulose 5-phosphate + formaldehyde | formaldehyde is the only acceptor | [Candida] boidinii | glyceraldehyde 3-phosphate + dihydroxyacetone | i.e. 1,3-dihydroxy-2-propanone | ir | |
D-xylulose 5-phosphate + formaldehyde | no acceptors are glyceraldehyde, glyceraldehyde 3-phosphate, erythrose 4-phosphate, ribose 5-phosphate, glucose 6-phosphate, D-ribose, glutaraldehyde, dihydroxyacetone | [Candida] boidinii | glyceraldehyde 3-phosphate + dihydroxyacetone | i.e. 1,3-dihydroxy-2-propanone | ir | |
D-xylulose 5-phosphate + formaldehyde | - |
[Candida] boidinii KD1 | glyceraldehyde 3-phosphate + dihydroxyacetone | i.e. 1,3-dihydroxy-2-propanone | ir | |
D-xylulose 5-phosphate + formaldehyde | formaldehyde is the only acceptor | [Candida] boidinii KD1 | glyceraldehyde 3-phosphate + dihydroxyacetone | i.e. 1,3-dihydroxy-2-propanone | ir | |
D-xylulose 5-phosphate + formaldehyde | no acceptors are glyceraldehyde, glyceraldehyde 3-phosphate, erythrose 4-phosphate, ribose 5-phosphate, glucose 6-phosphate, D-ribose, glutaraldehyde, dihydroxyacetone | [Candida] boidinii KD1 | glyceraldehyde 3-phosphate + dihydroxyacetone | i.e. 1,3-dihydroxy-2-propanone | ir | |
fructose 6-phosphate + formaldehyde | - |
[Candida] boidinii | dihydroxyacetone + erythrose 4-phosphate | - |
? | |
fructose 6-phosphate + formaldehyde | - |
[Candida] boidinii KD1 | dihydroxyacetone + erythrose 4-phosphate | - |
? | |
sedoheptulose 7-phosphate + formaldehyde | - |
[Candida] boidinii | dihydroxyacetone + ribose | - |
? | |
sedoheptulose 7-phosphate + formaldehyde | - |
[Candida] boidinii KD1 | dihydroxyacetone + ribose | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | alpha2, 2 * 76000, SDS-PAGE | [Candida] boidinii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
- |
[Candida] boidinii |
6.8 | 7.1 | - |
[Candida] boidinii |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
thiamine diphosphate | tightly bound | [Candida] boidinii | |
thiamine diphosphate | requirement, thiamine diphosphate protein, 0.5 mM | [Candida] boidinii |