BRENDA - Enzyme Database
show all sequences of 2.2.1.3

Purification and properties of dihydroxyacetone synthase from methylotrophic yeast Candida boidinii

Bystrykh, L.V.; Sokolov, A.P.; Trotsenko, Y.A.; FEBS Lett. 132, 324-328 (1981)
No PubMed abstract available

Data extracted from this reference:

General Stability
General Stability
Organism
Ca2+ does not stabilize
Candida boidinii
Mg2+ or NH4+ stabilizes for at least 80 h
Candida boidinii
Inhibitors
Inhibitors
Commentary
Organism
Structure
EDTA
-
Candida boidinii
formaldehyde
above 1 mM, xylulose 5-phosphate protects
Candida boidinii
GSH
-
Candida boidinii
NEM
irreversible, GSH partially protects
Candida boidinii
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Mg2+
5 mM, essential for effective binding of thiamine diphosphate to enzyme molecule
Candida boidinii
additional information
no activation by Ca2+
Candida boidinii
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
76000
-
alpha2, 2 * 76000, SDS-PAGE
Candida boidinii
145000
-
gel filtration
Candida boidinii
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
D-xylulose 5-phosphate + formaldehyde
Candida boidinii
-
glyceraldehyde 3-phosphate + dihydroxyacetone
i.e. 1,3-dihydroxy-2-propanone
Candida boidinii
ir
D-xylulose 5-phosphate + formaldehyde
Candida boidinii KD1
-
glyceraldehyde 3-phosphate + dihydroxyacetone
i.e. 1,3-dihydroxy-2-propanone
Candida boidinii KD1
ir
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Candida boidinii
-
; methanol grown
-
Candida boidinii KD1
-
-
-
Purification (Commentary)
Commentary
Organism
-
Candida boidinii
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
4
-
-
Candida boidinii
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D-xylulose 5-phosphate + formaldehyde
-
486050
Candida boidinii
glyceraldehyde 3-phosphate + dihydroxyacetone
i.e. 1,3-dihydroxy-2-propanone
486050
Candida boidinii
ir
D-xylulose 5-phosphate + formaldehyde
formaldehyde is the only acceptor
486050
Candida boidinii
glyceraldehyde 3-phosphate + dihydroxyacetone
i.e. 1,3-dihydroxy-2-propanone
486050
Candida boidinii
ir
D-xylulose 5-phosphate + formaldehyde
no acceptors are glyceraldehyde, glyceraldehyde 3-phosphate, erythrose 4-phosphate, ribose 5-phosphate, glucose 6-phosphate, D-ribose, glutaraldehyde, dihydroxyacetone
486050
Candida boidinii
glyceraldehyde 3-phosphate + dihydroxyacetone
i.e. 1,3-dihydroxy-2-propanone
486050
Candida boidinii
ir
D-xylulose 5-phosphate + formaldehyde
-
486050
Candida boidinii KD1
glyceraldehyde 3-phosphate + dihydroxyacetone
i.e. 1,3-dihydroxy-2-propanone
486050
Candida boidinii KD1
ir
D-xylulose 5-phosphate + formaldehyde
formaldehyde is the only acceptor
486050
Candida boidinii KD1
glyceraldehyde 3-phosphate + dihydroxyacetone
i.e. 1,3-dihydroxy-2-propanone
486050
Candida boidinii KD1
ir
D-xylulose 5-phosphate + formaldehyde
no acceptors are glyceraldehyde, glyceraldehyde 3-phosphate, erythrose 4-phosphate, ribose 5-phosphate, glucose 6-phosphate, D-ribose, glutaraldehyde, dihydroxyacetone
486050
Candida boidinii KD1
glyceraldehyde 3-phosphate + dihydroxyacetone
i.e. 1,3-dihydroxy-2-propanone
486050
Candida boidinii KD1
ir
fructose 6-phosphate + formaldehyde
-
486050
Candida boidinii
dihydroxyacetone + erythrose 4-phosphate
-
-
-
?
fructose 6-phosphate + formaldehyde
-
486050
Candida boidinii KD1
dihydroxyacetone + erythrose 4-phosphate
-
-
-
?
sedoheptulose 7-phosphate + formaldehyde
-
486050
Candida boidinii
dihydroxyacetone + ribose
-
-
-
-
sedoheptulose 7-phosphate + formaldehyde
-
486050
Candida boidinii KD1
dihydroxyacetone + ribose
-
-
-
-
Subunits
Subunits
Commentary
Organism
dimer
alpha2, 2 * 76000, SDS-PAGE
Candida boidinii
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
additional information
-
-
Candida boidinii
6.8
7.1
-
Candida boidinii
Cofactor
Cofactor
Commentary
Organism
Structure
thiamine diphosphate
requirement, thiamine diphosphate protein, 0.5 mM; tightly bound
Candida boidinii
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
thiamine diphosphate
requirement, thiamine diphosphate protein, 0.5 mM; tightly bound
Candida boidinii
General Stability (protein specific)
General Stability
Organism
Ca2+ does not stabilize
Candida boidinii
Mg2+ or NH4+ stabilizes for at least 80 h
Candida boidinii
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
EDTA
-
Candida boidinii
formaldehyde
above 1 mM, xylulose 5-phosphate protects
Candida boidinii
GSH
-
Candida boidinii
NEM
irreversible, GSH partially protects
Candida boidinii
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Mg2+
5 mM, essential for effective binding of thiamine diphosphate to enzyme molecule
Candida boidinii
additional information
no activation by Ca2+
Candida boidinii
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
76000
-
alpha2, 2 * 76000, SDS-PAGE
Candida boidinii
145000
-
gel filtration
Candida boidinii
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
D-xylulose 5-phosphate + formaldehyde
Candida boidinii
-
glyceraldehyde 3-phosphate + dihydroxyacetone
i.e. 1,3-dihydroxy-2-propanone
Candida boidinii
ir
D-xylulose 5-phosphate + formaldehyde
Candida boidinii KD1
-
glyceraldehyde 3-phosphate + dihydroxyacetone
i.e. 1,3-dihydroxy-2-propanone
Candida boidinii KD1
ir
Purification (Commentary) (protein specific)
Commentary
Organism
-
Candida boidinii
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
4
-
-
Candida boidinii
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D-xylulose 5-phosphate + formaldehyde
-
486050
Candida boidinii
glyceraldehyde 3-phosphate + dihydroxyacetone
i.e. 1,3-dihydroxy-2-propanone
486050
Candida boidinii
ir
D-xylulose 5-phosphate + formaldehyde
formaldehyde is the only acceptor
486050
Candida boidinii
glyceraldehyde 3-phosphate + dihydroxyacetone
i.e. 1,3-dihydroxy-2-propanone
486050
Candida boidinii
ir
D-xylulose 5-phosphate + formaldehyde
no acceptors are glyceraldehyde, glyceraldehyde 3-phosphate, erythrose 4-phosphate, ribose 5-phosphate, glucose 6-phosphate, D-ribose, glutaraldehyde, dihydroxyacetone
486050
Candida boidinii
glyceraldehyde 3-phosphate + dihydroxyacetone
i.e. 1,3-dihydroxy-2-propanone
486050
Candida boidinii
ir
D-xylulose 5-phosphate + formaldehyde
-
486050
Candida boidinii KD1
glyceraldehyde 3-phosphate + dihydroxyacetone
i.e. 1,3-dihydroxy-2-propanone
486050
Candida boidinii KD1
ir
D-xylulose 5-phosphate + formaldehyde
formaldehyde is the only acceptor
486050
Candida boidinii KD1
glyceraldehyde 3-phosphate + dihydroxyacetone
i.e. 1,3-dihydroxy-2-propanone
486050
Candida boidinii KD1
ir
D-xylulose 5-phosphate + formaldehyde
no acceptors are glyceraldehyde, glyceraldehyde 3-phosphate, erythrose 4-phosphate, ribose 5-phosphate, glucose 6-phosphate, D-ribose, glutaraldehyde, dihydroxyacetone
486050
Candida boidinii KD1
glyceraldehyde 3-phosphate + dihydroxyacetone
i.e. 1,3-dihydroxy-2-propanone
486050
Candida boidinii KD1
ir
fructose 6-phosphate + formaldehyde
-
486050
Candida boidinii
dihydroxyacetone + erythrose 4-phosphate
-
-
-
?
fructose 6-phosphate + formaldehyde
-
486050
Candida boidinii KD1
dihydroxyacetone + erythrose 4-phosphate
-
-
-
?
sedoheptulose 7-phosphate + formaldehyde
-
486050
Candida boidinii
dihydroxyacetone + ribose
-
-
-
-
sedoheptulose 7-phosphate + formaldehyde
-
486050
Candida boidinii KD1
dihydroxyacetone + ribose
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
alpha2, 2 * 76000, SDS-PAGE
Candida boidinii
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
additional information
-
-
Candida boidinii
6.8
7.1
-
Candida boidinii
Other publictions for EC 2.2.1.3
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
718524
Xiao
-
Overexpressions of dihydroxyac ...
Candida boidinii
Acta Physiol. Plant.
34
1975-1985
2012
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1
-
-
-
-
-
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-
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1
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1
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-
-
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-
688869
Seo
Cloning, characterization and ...
Mycobacterium sp., Mycobacterium sp. DSM 3803 / JC1
Microbiology
153
4174-4182
2007
-
-
1
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-
-
-
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1
2
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2
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2
1
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1
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1
2
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2
1
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-
-
486055
Stewart
Alcohol oxidase and dihydroxya ...
Candida boidinii
J. Cell Sci.
114
2863-2868
2001
-
-
-
-
-
-
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-
1
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1
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1
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1
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1
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-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
486056
Salomons
Overproduction of Pex5p stimul ...
Ogataea angusta
J. Biol. Chem.
275
12603-12611
2000
-
-
-
-
-
-
-
-
-
-
-
1
-
1
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-
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1
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1
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1
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-
-
-
-
486054
Sakai
Regulation and physiological r ...
Candida boidinii
J. Bacteriol.
180
5885-5890
1998
-
-
1
-
-
-
-
-
-
-
-
1
-
1
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-
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-
-
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1
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1
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1
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-
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-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
486057
Ro
Dihydroxyacetone synthase from ...
Acinetobacter sp.
J. Bacteriol.
179
6041-6047
1997
-
-
-
-
-
-
-
2
-
1
2
-
-
1
-
-
-
-
-
-
1
-
9
1
1
-
1
-
-
1
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
2
-
1
2
-
-
-
-
-
-
-
1
-
9
1
1
-
1
-
-
1
-
-
-
-
-
-
-
-
486053
Yanase
-
Enzymic preparation of [1,3-13 ...
Candida boidinii, Ogataea angusta
Appl. Microbiol. Biotechnol.
43
228-234
1995
-
-
-
-
-
-
-
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2
-
2
-
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1
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2
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2
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1
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2
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-
486048
Bystrykh
Dihydroxyacetone synthase from ...
Candida boidinii, Candida boidinii KD1, Ogataea angusta, Ogataea angusta DL-1
Methods Enzymol.
188
435-445
1990
-
-
-
-
-
4
9
-
2
3
2
4
-
10
-
-
2
-
-
-
1
4
19
1
-
-
-
-
2
-
-
1
-
-
-
-
-
-
1
-
-
4
-
9
-
-
2
3
2
4
-
-
-
2
-
-
1
4
19
1
-
-
-
-
2
-
-
-
-
-
-
-
-
-
486052
Goodman
Dihydroxyacetone synthase is a ...
Candida boidinii
J. Biol. Chem.
260
7108-7113
1985
-
-
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1
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2
1
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1
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1
1
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1
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2
1
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-
1
-
2
1
-
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1
1
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1
-
2
1
-
-
-
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-
-
-
-
-
-
-
-
486051
Waites
-
Dihydroxyacetone synthase: a s ...
Candida boidinii
J. Gen. Microbiol.
129
935-944
1983
-
-
-
-
-
-
5
-
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2
3
1
-
1
-
-
-
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1
-
14
1
-
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1
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-
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5
-
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2
3
1
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1
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14
1
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1
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-
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-
-
486047
Kato
Purification and properties of ...
Candida boidinii
Biochim. Biophys. Acta
715
143-150
1982
-
-
-
-
-
2
10
4
-
1
2
1
-
1
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1
-
-
-
1
2
4
1
1
1
2
-
1
1
-
1
-
-
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-
-
-
1
-
-
2
-
10
-
4
-
1
2
1
-
-
-
1
-
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1
2
4
1
1
1
2
-
1
1
-
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-
486046
Waites
The interrelation between tran ...
Candida boidinii
J. Gen. Microbiol.
124
309-316
1981
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2
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1
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1
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1
1
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1
2
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4
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2
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1
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1
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1
2
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4
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-
486050
Bystrykh
-
Purification and properties of ...
Candida boidinii, Candida boidinii KD1
FEBS Lett.
132
324-328
1981
-
-
-
-
-
2
4
-
-
2
2
2
-
4
-
-
1
-
-
-
1
-
10
1
-
-
-
-
2
-
-
1
-
-
-
-
-
-
1
-
-
2
-
4
-
-
-
2
2
2
-
-
-
1
-
-
1
-
10
1
-
-
-
-
2
-
-
-
-
-
-
-
-
-
486049
O'Connor
-
Pentose phosphate-dependent fi ...
Candida boidinii, Ogataea angusta, Ogataea angusta DL-1
J. Gen. Microbiol.
120
219-225
1980
-
-
-
-
-
2
-
-
-
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-
3
-
7
-
-
2
-
-
-
1
1
6
-
-
-
-
-
2
-
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-
-
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-
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-
-
2
-
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-
3
-
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2
-
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1
1
6
-
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-
2
-
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