Literature summary for 2.2.1.2 extracted from

Sautner, V.; Friedrich, M.M.; Lehwess-Litzmann, A.; Tittmann, K.
Converting transaldolase into aldolase through swapping of the multifunctional acid-base catalyst common and divergent catalytic principles in F6P aldolase and transaldolase (2015), Biochemistry, 54, 4475-4486 .

Search for more literature for 2.2.1.2

Crystallization (Commentary)

Crystallization (Comment)	Organism
mutant enzyme E60Q/F132Y, hanging drop vapor diffusion method, using 200 mM ammonium acetate (pH 4.4), 10% (w/v) PEG 6000, and 25% (v/v) glycerol	Thermoplasma acidophilum

Protein Variants

Protein Variants	Comment	Organism
E60Q/F132Y	the mutant enzyme has no transaldolase activity and shows D-fructose 6-phosphate aldolase activity	Thermoplasma acidophilum
F132Y	the mutant enzyme has no transaldolase activity and shows D-fructose 6-phosphate aldolase activity	Thermoplasma acidophilum

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
2.3	-	D-fructose 6-phosphate	wild type enzyme, at pH 7.5 and 30°C	Thermoplasma acidophilum

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
D-erythrose 4-phosphate + D-fructose 6-phosphate	Thermoplasma acidophilum	-	sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate	-	r
sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate	Thermoplasma acidophilum	-	D-erythrose 4-phosphate + D-fructose 6-phosphate	-	r

Organism

Organism	UniProt	Comment	Textmining
Thermoplasma acidophilum	Q9HKI3	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
D-erythrose 4-phosphate + D-fructose 6-phosphate	-	Thermoplasma acidophilum	sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate	-	r
additional information	the enzyme exhibits a very small yet detectable D-fructose 6-phosphate aldolase activity	Thermoplasma acidophilum	?	-	-
sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate	-	Thermoplasma acidophilum	D-erythrose 4-phosphate + D-fructose 6-phosphate	-	r

Synonyms

Synonyms	Comment	Organism
TAL	-	Thermoplasma acidophilum

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.24	-	D-fructose 6-phosphate	mutant enzyme F132Y, at pH 7.5 and 30°C	Thermoplasma acidophilum
0.62	-	D-fructose 6-phosphate	mutant enzyme E60Q/F132Y, at pH 7.5 and 30°C	Thermoplasma acidophilum
13.86	-	D-fructose 6-phosphate	wild type enzyme, at pH 7.5 and 30°C	Thermoplasma acidophilum

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
6.0261	-	D-fructose 6-phosphate	wild type enzyme, at pH 7.5 and 30°C	Thermoplasma acidophilum

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Release 2023.1 (January 2023)