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Literature summary for 2.2.1.2 extracted from

  • Huang, S.Y.; Zhang, Y.H.; Zhong, J.J.
    A thermostable recombinant transaldolase with high activity over a broad pH range (2012), Appl. Microbiol. Biotechnol., 93, 2403-2410.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Thermotoga maritima

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.27
-
D-erythrose 4-phosphate pH 9.0, 80°C Thermotoga maritima
0.36
-
D-fructose 6-phosphate pH 9.0, 80°C Thermotoga maritima

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
24000
-
x * 24000, SDS-PAGE, x * 24213, calculated Thermotoga maritima
24213
-
x * 24000, SDS-PAGE, x * 24213, calculated Thermotoga maritima

Organism

Organism UniProt Comment Textmining
Thermotoga maritima Q9WYD1
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
8.9
-
30°C, pH 9.0 Thermotoga maritima
42
-
80°C, pH 9.0 Thermotoga maritima

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-erythrose 4-phosphate + D-fructose 6-phosphate
-
Thermotoga maritima sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate
-
?

Subunits

Subunits Comment Organism
? x * 24000, SDS-PAGE, x * 24213, calculated Thermotoga maritima

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
80
-
-
Thermotoga maritima

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
40
-
28% of maximum activity Thermotoga maritima
60
-
73% of maximum activity Thermotoga maritima
90
-
96% of maximum activity Thermotoga maritima

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
half life 198 h Thermotoga maritima
80
-
half-life 13 h Thermotoga maritima

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
22.3
-
D-fructose 6-phosphate pH 9.0, 80°C Thermotoga maritima
22.9
-
D-erythrose 4-phosphate pH 9.0, 80°C Thermotoga maritima

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
in glycine-NaOH buffer Thermotoga maritima

pH Range

pH Minimum pH Maximum Comment Organism
6 9
-
Thermotoga maritima