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Literature summary for 2.2.1.11 extracted from

  • White, R.H.; Xu, H.
    Methylglyoxal is an intermediate in the biosynthesis of 6-deoxy-5-ketofructose-1-phosphate: a precursor for aromatic amino acid biosynthesis in Methanocaldococcus jannaschii (2006), Biochemistry, 45, 12366-12379.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene MJ1585, expression in Escherichia coli using primersforward 5'-GGTCATATGGGGATTTTTATG-3' and reverse 5'-GCTGGATCCTTATTTCCTATCTC-3' Methanocaldococcus jannaschii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
methylglyoxal + D-fructose 1,6-bisphosphate Methanocaldococcus jannaschii
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D-glyceraldehyde 3-phosphate + 1-deoxy-D-threo-hexo-2,5-diulose 6-phosphate
-
?
methylglyoxal + D-fructose 1-phosphate Methanocaldococcus jannaschii
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D-glyceraldehyde + 1-deoxy-D-threo-hexo-2,5-diulose 6-phosphate
-
?
additional information Methanocaldococcus jannaschii the enzyme catalyzes the condensation of methylglyoxal with a dihydroxyacetone-phosphate fragment, which is derived from fructose 1-phosphate and/or fructose 1,6-bisphosphate, generating 6-deoxy-5-ketofructose 1-phosphate ?
-
?

Organism

Organism UniProt Comment Textmining
Methanocaldococcus jannaschii Q58980 gene MJ1585
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
methylglyoxal + D-fructose 1,6-bisphosphate
-
Methanocaldococcus jannaschii D-glyceraldehyde 3-phosphate + 1-deoxy-D-threo-hexo-2,5-diulose 6-phosphate
-
?
methylglyoxal + D-fructose 1-phosphate
-
Methanocaldococcus jannaschii D-glyceraldehyde + 1-deoxy-D-threo-hexo-2,5-diulose 6-phosphate
-
?
additional information the enzyme catalyzes the condensation of methylglyoxal with a dihydroxyacetone-phosphate fragment, which is derived from fructose 1-phosphate and/or fructose 1,6-bisphosphate, generating 6-deoxy-5-ketofructose 1-phosphate Methanocaldococcus jannaschii ?
-
?

Synonyms

Synonyms Comment Organism
MJ1585
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Methanocaldococcus jannaschii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
70
-
assay at Methanocaldococcus jannaschii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Methanocaldococcus jannaschii

General Information

General Information Comment Organism
metabolism the enzyme is required in the biosynthetic pathway for creating 6-deoxy-5-ketofructose 1-phosphate, DKFP, a precursor sugar for aromatic amino acid biosynthesis in Methanocaldococcus jannaschii. This pathway is not leading via the nucleoside diphosphate mannose pathway nor a pathway involving nucleoside diphosphate derivatives of fructose 1-phosphate, fructose 2-phosphate, or fructose 1,6-bisphosphate, but methylglyoxal and a fructose 1-phosphate- or fructose 1,6-bisphosphate-derived dihydroxyacetone phosphate fragment are key intermediates in DKFP production, detailed overview. The stereochemistry of the aldolase reaction is consistent with MJ1585 also functioning as the fructose 1,6-bisphosphate aldolase, EC 4.1.2.13, used in glycolysis. The MJ1585 aldolase does not catalyze the reaction of DL-lactaldehyde with fructose 1-phosphate, fructose 1,6-phosphate, or DHAP to form 6-deoxyallulose 1-phosphate or fuculose 1-phosphate Methanocaldococcus jannaschii