Literature summary for 2.2.1.1 extracted from

Shaw, J.A.; Henard, C.A.; Liu, L.; Dieckman, L.M.; Vazquez-Torres, A.; Bourret, T.J.
Salmonella enterica serovar Typhimurium has three transketolase enzymes contributing to the pentose phosphate pathway (2018), J. Biol. Chem., 293, 11271-11282 .

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KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.023	-	D-erythrose 4-phosphate	with excess D-xylose 5-phosphate as cosubstrate, at pH 7.5 and 37°C	Salmonella enterica subsp. enterica serovar Typhimurium
0.058	-	D-ribose 5-phosphate	with excess D-xylose 5-phosphate as cosubstrate, at pH 7.5 and 37°C	Salmonella enterica subsp. enterica serovar Typhimurium
0.082	-	D-erythrose 4-phosphate	with excess D-xylose 5-phosphate as cosubstrate, at pH 7.5 and 37°C	Salmonella enterica subsp. enterica serovar Typhimurium
0.099	-	D-erythrose 4-phosphate	with excess D-xylose 5-phosphate as cosubstrate, at pH 7.5 and 37°C	Salmonella enterica subsp. enterica serovar Typhimurium
0.12	-	D-xylulose 5-phosphate	with excess D-erythrose 4-phosphate as cosubstrate, at pH 7.5 and 37°C	Salmonella enterica subsp. enterica serovar Typhimurium
0.17	-	D-xylulose 5-phosphate	with excess D-erythrose 4-phosphate as cosubstrate, at pH 7.5 and 37°C	Salmonella enterica subsp. enterica serovar Typhimurium
0.18	-	D-xylulose 5-phosphate	with excess D-erythrose 4-phosphate as cosubstrate, at pH 7.5 and 37°C	Salmonella enterica subsp. enterica serovar Typhimurium
0.27	-	D-xylulose 5-phosphate	with excess D-ribose 5-phosphate as cosubstrate, at pH 7.5 and 37°C	Salmonella enterica subsp. enterica serovar Typhimurium
0.29	-	D-xylulose 5-phosphate	with excess D-ribose 5-phosphate as cosubstrate, at pH 7.5 and 37°C	Salmonella enterica subsp. enterica serovar Typhimurium
0.66	-	D-ribose 5-phosphate	with excess D-xylose 5-phosphate as cosubstrate, at pH 7.5 and 37°C	Salmonella enterica subsp. enterica serovar Typhimurium
0.73	-	D-ribose 5-phosphate	with excess D-xylose 5-phosphate as cosubstrate, at pH 7.5 and 37°C	Salmonella enterica subsp. enterica serovar Typhimurium
0.75	-	D-xylulose 5-phosphate	with excess D-ribose 5-phosphate as cosubstrate, at pH 7.5 and 37°C	Salmonella enterica subsp. enterica serovar Typhimurium

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
D-erythrose 4-phosphate + D-xylulose 5-phosphate	Salmonella enterica subsp. enterica serovar Typhimurium	-	?	-	r
D-erythrose 4-phosphate + D-xylulose 5-phosphate	Salmonella enterica subsp. enterica serovar Typhimurium ATCC 14028s	-	?	-	r
D-ribose 5-phosphate + D-xylulose 5-phosphate	Salmonella enterica subsp. enterica serovar Typhimurium	-	sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate	-	r
D-ribose 5-phosphate + D-xylulose 5-phosphate	Salmonella enterica subsp. enterica serovar Typhimurium ATCC 14028s	-	sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate	-	r
sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate	Salmonella enterica subsp. enterica serovar Typhimurium	-	D-ribose 5-phosphate + D-xylulose 5-phosphate	-	r
sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate	Salmonella enterica subsp. enterica serovar Typhimurium ATCC 14028s	-	D-ribose 5-phosphate + D-xylulose 5-phosphate	-	r

Organism

Organism	UniProt	Comment	Textmining
Salmonella enterica subsp. enterica serovar Typhimurium	A0A0F6B484 and A0A0F6B483	-	-
Salmonella enterica subsp. enterica serovar Typhimurium	A0A0F6B4M5	-	-
Salmonella enterica subsp. enterica serovar Typhimurium	A0A0F6B6H7	-	-
Salmonella enterica subsp. enterica serovar Typhimurium ATCC 14028s	A0A0F6B484 and A0A0F6B483	-	-
Salmonella enterica subsp. enterica serovar Typhimurium ATCC 14028s	A0A0F6B4M5	-	-
Salmonella enterica subsp. enterica serovar Typhimurium ATCC 14028s	A0A0F6B6H7	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
D-erythrose 4-phosphate + D-xylulose 5-phosphate	-	Salmonella enterica subsp. enterica serovar Typhimurium	?	-	r
D-erythrose 4-phosphate + D-xylulose 5-phosphate	-	Salmonella enterica subsp. enterica serovar Typhimurium ATCC 14028s	?	-	r
D-ribose 5-phosphate + D-xylulose 5-phosphate	-	Salmonella enterica subsp. enterica serovar Typhimurium	sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate	-	r
D-ribose 5-phosphate + D-xylulose 5-phosphate	-	Salmonella enterica subsp. enterica serovar Typhimurium ATCC 14028s	sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate	-	r
additional information	isoform TktA is responsible for more than 88% of the transketolase activity in wild type cells	Salmonella enterica subsp. enterica serovar Typhimurium	?	-	-
additional information	isoform TktA is responsible for more than 88% of the transketolase activity in wild type cells	Salmonella enterica subsp. enterica serovar Typhimurium ATCC 14028s	?	-	-
sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate	-	Salmonella enterica subsp. enterica serovar Typhimurium	D-ribose 5-phosphate + D-xylulose 5-phosphate	-	r
sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate	-	Salmonella enterica subsp. enterica serovar Typhimurium ATCC 14028s	D-ribose 5-phosphate + D-xylulose 5-phosphate	-	r

Subunits

Subunits	Comment	Organism
?	x * 34000, isoform TktC, SDS-PAGE	Salmonella enterica subsp. enterica serovar Typhimurium
?	x * 72000, isoform TktA, SDS-PAGE	Salmonella enterica subsp. enterica serovar Typhimurium
?	x * 73000, isoform TktB, SDS-PAGE	Salmonella enterica subsp. enterica serovar Typhimurium

Synonyms

Synonyms	Comment	Organism
STM14_2885	-	Salmonella enterica subsp. enterica serovar Typhimurium
STM14_2886	-	Salmonella enterica subsp. enterica serovar Typhimurium
TktA	isoform	Salmonella enterica subsp. enterica serovar Typhimurium
TktB	isoform	Salmonella enterica subsp. enterica serovar Typhimurium
TKTc	isoform	Salmonella enterica subsp. enterica serovar Typhimurium

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
671	-	D-xylulose 5-phosphate	with excess D-erythrose 4-phosphate as cosubstrate, at pH 7.5 and 37°C	Salmonella enterica subsp. enterica serovar Typhimurium
816	-	D-xylulose 5-phosphate	with excess D-ribose 5-phosphate as cosubstrate, at pH 7.5 and 37°C	Salmonella enterica subsp. enterica serovar Typhimurium
1350	-	D-ribose 5-phosphate	with excess D-xylose 5-phosphate as cosubstrate, at pH 7.5 and 37°C	Salmonella enterica subsp. enterica serovar Typhimurium
1390	-	D-erythrose 4-phosphate	with excess D-xylose 5-phosphate as cosubstrate, at pH 7.5 and 37°C	Salmonella enterica subsp. enterica serovar Typhimurium
52500	-	D-xylulose 5-phosphate	with excess D-erythrose 4-phosphate as cosubstrate, at pH 7.5 and 37°C	Salmonella enterica subsp. enterica serovar Typhimurium
67100	-	D-ribose 5-phosphate	with excess D-xylose 5-phosphate as cosubstrate, at pH 7.5 and 37°C	Salmonella enterica subsp. enterica serovar Typhimurium
71400	-	D-xylulose 5-phosphate	with excess D-erythrose 4-phosphate as cosubstrate, at pH 7.5 and 37°C	Salmonella enterica subsp. enterica serovar Typhimurium
78500	-	D-xylulose 5-phosphate	with excess D-ribose 5-phosphate as cosubstrate, at pH 7.5 and 37°C	Salmonella enterica subsp. enterica serovar Typhimurium
86500	-	D-erythrose 4-phosphate	with excess D-xylose 5-phosphate as cosubstrate, at pH 7.5 and 37°C	Salmonella enterica subsp. enterica serovar Typhimurium
95400	-	D-ribose 5-phosphate	with excess D-xylose 5-phosphate as cosubstrate, at pH 7.5 and 37°C	Salmonella enterica subsp. enterica serovar Typhimurium
104000	-	D-erythrose 4-phosphate	with excess D-xylose 5-phosphate as cosubstrate, at pH 7.5 and 37°C	Salmonella enterica subsp. enterica serovar Typhimurium
131000	-	D-xylulose 5-phosphate	with excess D-ribose 5-phosphate as cosubstrate, at pH 7.5 and 37°C	Salmonella enterica subsp. enterica serovar Typhimurium

Cofactor

Cofactor	Comment	Organism	Structure
thiamine diphosphate	-	Salmonella enterica subsp. enterica serovar Typhimurium

General Information

General Information	Comment	Organism
malfunction	transketolase-deficient Salmonella enterica Typhimurium can grow with glucose, but not ribose, as the sole carbon source	Salmonella enterica subsp. enterica serovar Typhimurium

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
5580	-	D-xylulose 5-phosphate	with excess D-erythrose 4-phosphate as cosubstrate, at pH 7.5 and 37°C	Salmonella enterica subsp. enterica serovar Typhimurium
10800	-	D-xylulose 5-phosphate	with excess D-ribose 5-phosphate as cosubstrate, at pH 7.5 and 37°C	Salmonella enterica subsp. enterica serovar Typhimurium
23200	-	D-ribose 5-phosphate	with excess D-xylose 5-phosphate as cosubstrate, at pH 7.5 and 37°C	Salmonella enterica subsp. enterica serovar Typhimurium
60700	-	D-erythrose 4-phosphate	with excess D-xylose 5-phosphate as cosubstrate, at pH 7.5 and 37°C	Salmonella enterica subsp. enterica serovar Typhimurium
102000	-	D-ribose 5-phosphate	with excess D-xylose 5-phosphate as cosubstrate, at pH 7.5 and 37°C	Salmonella enterica subsp. enterica serovar Typhimurium
106000	-	D-erythrose 4-phosphate	with excess D-xylose 5-phosphate as cosubstrate, at pH 7.5 and 37°C	Salmonella enterica subsp. enterica serovar Typhimurium
131000	-	D-ribose 5-phosphate	with excess D-xylose 5-phosphate as cosubstrate, at pH 7.5 and 37°C	Salmonella enterica subsp. enterica serovar Typhimurium
269000	-	D-xylulose 5-phosphate	with excess D-ribose 5-phosphate as cosubstrate, at pH 7.5 and 37°C	Salmonella enterica subsp. enterica serovar Typhimurium
299000	-	D-xylulose 5-phosphate	with excess D-erythrose 4-phosphate as cosubstrate, at pH 7.5 and 37°C	Salmonella enterica subsp. enterica serovar Typhimurium
422000	-	D-xylulose 5-phosphate	with excess D-erythrose 4-phosphate as cosubstrate, at pH 7.5 and 37°C	Salmonella enterica subsp. enterica serovar Typhimurium
477000	-	D-xylulose 5-phosphate	with excess D-ribose 5-phosphate as cosubstrate, at pH 7.5 and 37°C	Salmonella enterica subsp. enterica serovar Typhimurium
1050000	-	D-erythrose 4-phosphate	with excess D-xylose 5-phosphate as cosubstrate, at pH 7.5 and 37°C	Salmonella enterica subsp. enterica serovar Typhimurium

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Release 2023.1 (January 2023)