Protein Variants | Comment | Organism |
---|---|---|
D469T | the mutant shows higher activity with 3-formylbenzoic acid and 4-formylbenzoic acid compared to the wild type enzyme | Escherichia coli |
D469T/R520Q | the mutant shows 80% activity with 3-formylbenzoic acid, 50% activity with 4-formylbenzoic acid and no activity with 3-hydroxybenzaldehyde compared to mutant enzyme D469T | Escherichia coli |
S385E | the mutation completely removes the substrate inhibition for 3-formylbenzoic acid | Escherichia coli |
S385E/D469T/R520Q | the mutant shows 350% activity with 3-formylbenzoic acid, 20% activity with 4-formylbenzoic acid and 600% activity activity with 3-hydroxybenzaldehyde compared to mutant enzyme D469T | Escherichia coli |
S385T/D469T/R520Q | the mutant shows 50% activity with 3-formylbenzoic acid, 210% activity with 4-formylbenzoic acid and 1270% activity activity with 3-hydroxybenzaldehyde compared to mutant enzyme D469T | Escherichia coli |
S385Y/D469T/R520Q | the mutant shows 50% activity with 3-formylbenzoic acid, 340% activity with 4-formylbenzoic acid and 1240% activity activity with 3-hydroxybenzaldehyde compared to mutant enzyme D469T | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
3-formylbenzoic acid | substrate inhibition | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.3 | - |
3-formylbenzoic acid | mutant enzyme S385Y/D469T/R520Q, at pH 7.0 and 22°C | Escherichia coli | |
1.7 | - |
3-formylbenzoic acid | mutant enzyme S385T/D469T/R520Q, at pH 7.0 and 22°C | Escherichia coli | |
2 | 3 | 4-formylbenzoic acid | mutant enzyme S385T/D469T/R520Q, at pH 7.0 and 22°C | Escherichia coli | |
13 | - |
3-formylbenzoic acid | mutant enzyme D469T/R520Q, at pH 7.0 and 22°C | Escherichia coli | |
13 | - |
4-formylbenzoic acid | mutant enzyme S385Y/D469T/R520Q, at pH 7.0 and 22°C | Escherichia coli | |
18 | - |
3-formylbenzoic acid | mutant enzyme S385E/D469T/R520Q, at pH 7.0 and 22°C | Escherichia coli | |
25 | - |
4-formylbenzoic acid | mutant enzyme D469T/R520Q, at pH 7.0 and 22°C | Escherichia coli | |
56 | - |
3-formylbenzoic acid | mutant enzyme D469T, at pH 7.0 and 22°C | Escherichia coli | |
72 | - |
4-formylbenzoic acid | mutant enzyme S385E/D469T/R520Q, at pH 7.0 and 22°C | Escherichia coli | |
180 | - |
3-Hydroxybenzaldehyde | mutant enzyme S385T/D469T/R520Q, at pH 7.0 and 22°C | Escherichia coli | |
245 | - |
3-Hydroxybenzaldehyde | mutant enzyme S385E/D469T/R520Q, at pH 7.0 and 22°C | Escherichia coli | |
251 | - |
4-formylbenzoic acid | mutant enzyme D469T, at pH 7.0 and 22°C | Escherichia coli | |
390 | - |
3-Hydroxybenzaldehyde | mutant enzyme S385Y/D469T/R520Q, at pH 7.0 and 22°C | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-formylbenzoic acid + hydroxypyruvate | - |
Escherichia coli | 3-(1,3-dihydroxy-2-oxopropyl)benzoic acid + CO2 | - |
? | |
3-hydroxybenzaldehyde + hydroxypyruvate | - |
Escherichia coli | 1,3-dihydroxy-1-(3-hydroxyphenyl)propan-2-one + CO2 | - |
? | |
4-formylbenzoic acid + hydroxypyruvate | - |
Escherichia coli | 4-(1,3-dihydroxy-2-oxopropyl)benzoic acid + CO2 | - |
? | |
propanal + glycolaldehyde | - |
Escherichia coli | (3S)-1,3-dihydroxypentan-2-one | - |
? |
Synonyms | Comment | Organism |
---|---|---|
TktA | - |
Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.2 | - |
4-formylbenzoic acid | mutant enzyme D469T/R520Q, at pH 7.0 and 22°C | Escherichia coli | |
0.3 | - |
4-formylbenzoic acid | mutant enzyme S385E/D469T/R520Q, at pH 7.0 and 22°C | Escherichia coli | |
0.6 | - |
3-Hydroxybenzaldehyde | mutant enzyme S385E/D469T/R520Q, at pH 7.0 and 22°C | Escherichia coli | |
1 | - |
3-Hydroxybenzaldehyde | mutant enzyme S385T/D469T/R520Q, at pH 7.0 and 22°C | Escherichia coli | |
1.7 | - |
4-formylbenzoic acid | mutant enzyme S385Y/D469T/R520Q, at pH 7.0 and 22°C | Escherichia coli | |
2.1 | - |
4-formylbenzoic acid | mutant enzyme S385T/D469T/R520Q, at pH 7.0 and 22°C | Escherichia coli | |
2.1 | - |
3-Hydroxybenzaldehyde | mutant enzyme S385Y/D469T/R520Q, at pH 7.0 and 22°C | Escherichia coli | |
5 | - |
4-formylbenzoic acid | mutant enzyme D469T, at pH 7.0 and 22°C | Escherichia coli | |
6 | - |
3-formylbenzoic acid | mutant enzyme D469T/R520Q, at pH 7.0 and 22°C | Escherichia coli | |
7 | - |
3-formylbenzoic acid | mutant enzyme S385Y/D469T/R520Q, at pH 7.0 and 22°C | Escherichia coli | |
7.3 | - |
3-formylbenzoic acid | mutant enzyme S385T/D469T/R520Q, at pH 7.0 and 22°C | Escherichia coli | |
13.2 | - |
3-formylbenzoic acid | mutant enzyme D469T, at pH 7.0 and 22°C | Escherichia coli | |
34 | - |
3-formylbenzoic acid | mutant enzyme S385E/D469T/R520Q, at pH 7.0 and 22°C | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
thiamine diphosphate | - |
Escherichia coli |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0024 | - |
3-Hydroxybenzaldehyde | mutant enzyme S385E/D469T/R520Q, at pH 7.0 and 22°C | Escherichia coli | |
0.0046 | - |
4-formylbenzoic acid | mutant enzyme S385E/D469T/R520Q, at pH 7.0 and 22°C | Escherichia coli | |
0.0054 | - |
3-Hydroxybenzaldehyde | mutant enzyme S385Y/D469T/R520Q, at pH 7.0 and 22°C | Escherichia coli | |
0.0055 | - |
3-Hydroxybenzaldehyde | mutant enzyme S385T/D469T/R520Q, at pH 7.0 and 22°C | Escherichia coli | |
0.0081 | - |
4-formylbenzoic acid | mutant enzyme D469T/R520Q, at pH 7.0 and 22°C | Escherichia coli | |
0.02 | - |
4-formylbenzoic acid | mutant enzyme D469T, at pH 7.0 and 22°C | Escherichia coli | |
0.09 | - |
4-formylbenzoic acid | mutant enzyme S385T/D469T/R520Q, at pH 7.0 and 22°C | Escherichia coli | |
0.11 | - |
4-formylbenzoic acid | mutant enzyme S385Y/D469T/R520Q, at pH 7.0 and 22°C | Escherichia coli | |
0.24 | - |
3-formylbenzoic acid | mutant enzyme D469T, at pH 7.0 and 22°C | Escherichia coli | |
0.47 | - |
3-formylbenzoic acid | mutant enzyme D469T/R520Q, at pH 7.0 and 22°C | Escherichia coli | |
1.9 | - |
3-formylbenzoic acid | mutant enzyme S385E/D469T/R520Q, at pH 7.0 and 22°C | Escherichia coli | |
4.25 | - |
3-formylbenzoic acid | mutant enzyme S385T/D469T/R520Q, at pH 7.0 and 22°C | Escherichia coli | |
5.4 | - |
3-formylbenzoic acid | mutant enzyme S385Y/D469T/R520Q, at pH 7.0 and 22°C | Escherichia coli |