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Literature summary for 2.2.1.1 extracted from

  • Payongsri, P.; Steadman, D.; Hailes, H.C.; Dalby, P.A.
    Second generation engineering of transketolase for polar aromatic aldehyde substrates (2015), Enzyme Microb. Technol., 71, 45-52 .
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
D469T the mutant shows higher activity with 3-formylbenzoic acid and 4-formylbenzoic acid compared to the wild type enzyme Escherichia coli
D469T/R520Q the mutant shows 80% activity with 3-formylbenzoic acid, 50% activity with 4-formylbenzoic acid and no activity with 3-hydroxybenzaldehyde compared to mutant enzyme D469T Escherichia coli
S385E the mutation completely removes the substrate inhibition for 3-formylbenzoic acid Escherichia coli
S385E/D469T/R520Q the mutant shows 350% activity with 3-formylbenzoic acid, 20% activity with 4-formylbenzoic acid and 600% activity activity with 3-hydroxybenzaldehyde compared to mutant enzyme D469T Escherichia coli
S385T/D469T/R520Q the mutant shows 50% activity with 3-formylbenzoic acid, 210% activity with 4-formylbenzoic acid and 1270% activity activity with 3-hydroxybenzaldehyde compared to mutant enzyme D469T Escherichia coli
S385Y/D469T/R520Q the mutant shows 50% activity with 3-formylbenzoic acid, 340% activity with 4-formylbenzoic acid and 1240% activity activity with 3-hydroxybenzaldehyde compared to mutant enzyme D469T Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
3-formylbenzoic acid substrate inhibition Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3
-
3-formylbenzoic acid mutant enzyme S385Y/D469T/R520Q, at pH 7.0 and 22°C Escherichia coli
1.7
-
3-formylbenzoic acid mutant enzyme S385T/D469T/R520Q, at pH 7.0 and 22°C Escherichia coli
2 3 4-formylbenzoic acid mutant enzyme S385T/D469T/R520Q, at pH 7.0 and 22°C Escherichia coli
13
-
3-formylbenzoic acid mutant enzyme D469T/R520Q, at pH 7.0 and 22°C Escherichia coli
13
-
4-formylbenzoic acid mutant enzyme S385Y/D469T/R520Q, at pH 7.0 and 22°C Escherichia coli
18
-
3-formylbenzoic acid mutant enzyme S385E/D469T/R520Q, at pH 7.0 and 22°C Escherichia coli
25
-
4-formylbenzoic acid mutant enzyme D469T/R520Q, at pH 7.0 and 22°C Escherichia coli
56
-
3-formylbenzoic acid mutant enzyme D469T, at pH 7.0 and 22°C Escherichia coli
72
-
4-formylbenzoic acid mutant enzyme S385E/D469T/R520Q, at pH 7.0 and 22°C Escherichia coli
180
-
3-Hydroxybenzaldehyde mutant enzyme S385T/D469T/R520Q, at pH 7.0 and 22°C Escherichia coli
245
-
3-Hydroxybenzaldehyde mutant enzyme S385E/D469T/R520Q, at pH 7.0 and 22°C Escherichia coli
251
-
4-formylbenzoic acid mutant enzyme D469T, at pH 7.0 and 22°C Escherichia coli
390
-
3-Hydroxybenzaldehyde mutant enzyme S385Y/D469T/R520Q, at pH 7.0 and 22°C Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-formylbenzoic acid + hydroxypyruvate
-
Escherichia coli 3-(1,3-dihydroxy-2-oxopropyl)benzoic acid + CO2
-
?
3-hydroxybenzaldehyde + hydroxypyruvate
-
Escherichia coli 1,3-dihydroxy-1-(3-hydroxyphenyl)propan-2-one + CO2
-
?
4-formylbenzoic acid + hydroxypyruvate
-
Escherichia coli 4-(1,3-dihydroxy-2-oxopropyl)benzoic acid + CO2
-
?
propanal + glycolaldehyde
-
Escherichia coli (3S)-1,3-dihydroxypentan-2-one
-
?

Synonyms

Synonyms Comment Organism
TktA
-
Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.2
-
4-formylbenzoic acid mutant enzyme D469T/R520Q, at pH 7.0 and 22°C Escherichia coli
0.3
-
4-formylbenzoic acid mutant enzyme S385E/D469T/R520Q, at pH 7.0 and 22°C Escherichia coli
0.6
-
3-Hydroxybenzaldehyde mutant enzyme S385E/D469T/R520Q, at pH 7.0 and 22°C Escherichia coli
1
-
3-Hydroxybenzaldehyde mutant enzyme S385T/D469T/R520Q, at pH 7.0 and 22°C Escherichia coli
1.7
-
4-formylbenzoic acid mutant enzyme S385Y/D469T/R520Q, at pH 7.0 and 22°C Escherichia coli
2.1
-
4-formylbenzoic acid mutant enzyme S385T/D469T/R520Q, at pH 7.0 and 22°C Escherichia coli
2.1
-
3-Hydroxybenzaldehyde mutant enzyme S385Y/D469T/R520Q, at pH 7.0 and 22°C Escherichia coli
5
-
4-formylbenzoic acid mutant enzyme D469T, at pH 7.0 and 22°C Escherichia coli
6
-
3-formylbenzoic acid mutant enzyme D469T/R520Q, at pH 7.0 and 22°C Escherichia coli
7
-
3-formylbenzoic acid mutant enzyme S385Y/D469T/R520Q, at pH 7.0 and 22°C Escherichia coli
7.3
-
3-formylbenzoic acid mutant enzyme S385T/D469T/R520Q, at pH 7.0 and 22°C Escherichia coli
13.2
-
3-formylbenzoic acid mutant enzyme D469T, at pH 7.0 and 22°C Escherichia coli
34
-
3-formylbenzoic acid mutant enzyme S385E/D469T/R520Q, at pH 7.0 and 22°C Escherichia coli

Cofactor

Cofactor Comment Organism Structure
thiamine diphosphate
-
Escherichia coli

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.0024
-
3-Hydroxybenzaldehyde mutant enzyme S385E/D469T/R520Q, at pH 7.0 and 22°C Escherichia coli
0.0046
-
4-formylbenzoic acid mutant enzyme S385E/D469T/R520Q, at pH 7.0 and 22°C Escherichia coli
0.0054
-
3-Hydroxybenzaldehyde mutant enzyme S385Y/D469T/R520Q, at pH 7.0 and 22°C Escherichia coli
0.0055
-
3-Hydroxybenzaldehyde mutant enzyme S385T/D469T/R520Q, at pH 7.0 and 22°C Escherichia coli
0.0081
-
4-formylbenzoic acid mutant enzyme D469T/R520Q, at pH 7.0 and 22°C Escherichia coli
0.02
-
4-formylbenzoic acid mutant enzyme D469T, at pH 7.0 and 22°C Escherichia coli
0.09
-
4-formylbenzoic acid mutant enzyme S385T/D469T/R520Q, at pH 7.0 and 22°C Escherichia coli
0.11
-
4-formylbenzoic acid mutant enzyme S385Y/D469T/R520Q, at pH 7.0 and 22°C Escherichia coli
0.24
-
3-formylbenzoic acid mutant enzyme D469T, at pH 7.0 and 22°C Escherichia coli
0.47
-
3-formylbenzoic acid mutant enzyme D469T/R520Q, at pH 7.0 and 22°C Escherichia coli
1.9
-
3-formylbenzoic acid mutant enzyme S385E/D469T/R520Q, at pH 7.0 and 22°C Escherichia coli
4.25
-
3-formylbenzoic acid mutant enzyme S385T/D469T/R520Q, at pH 7.0 and 22°C Escherichia coli
5.4
-
3-formylbenzoic acid mutant enzyme S385Y/D469T/R520Q, at pH 7.0 and 22°C Escherichia coli