Protein Variants | Comment | Organism |
---|---|---|
D477A | the mutant shows a kcat/KM value decrease for (2R)-hydroxylated aldehydes decrease by three orders of magnitude compared to the wild type enzyme | Geobacillus stearothermophilus |
L382D/D470S | the mutant leads up to 4 and 5fold higher activities towards L-lactaldehyde and L-glyceraldehyde, respectively, compared to the wild type enzyme | Geobacillus stearothermophilus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
6 | - |
D-glyceraldehyde | at pH 7.2 and 50°C | Geobacillus stearothermophilus | |
8.4 | - |
D-Lactaldehyde | at pH 7.2 and 50°C | Geobacillus stearothermophilus | |
300 | - |
L-glyceraldehyde | Km above 300 mM, at pH 7.2 and 50°C | Geobacillus stearothermophilus | |
300 | - |
L-lactaldehyde | Km above 300 mM, at pH 7.2 and 50°C | Geobacillus stearothermophilus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Geobacillus stearothermophilus | A0A0I9QGZ2 | - |
- |
Storage Stability | Organism |
---|---|
50°C, 7 days, retains 100% activity | Geobacillus stearothermophilus |
65°C, 3 days, retains 100% activity | Geobacillus stearothermophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glyceraldehyde + hydroxypyruvate | - |
Geobacillus stearothermophilus | D-ribulose + ? | - |
? | |
D-lactaldehyde + hydroxypyruvate | - |
Geobacillus stearothermophilus | 5-deoxy-D-ribulose + ? | - |
? | |
L-glyceraldehyde + hydroxypyruvate | minor reactivity | Geobacillus stearothermophilus | L-ribulose + ? | - |
? | |
L-lactaldehyde + hydroxypyruvate | minor reactivity | Geobacillus stearothermophilus | 5-deoxy-L-ribulose + ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
- |
Geobacillus stearothermophilus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
thiamine diphosphate | - |
Geobacillus stearothermophilus |