Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
additional information | in vitro induction of the expression by arabinose decreases the amount of recombinant enzyme isolated and lowers its specific activity | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.011 | - |
D-xylulose 5-phosphate | pH 7.6, 22°C | Homo sapiens | |
0.063 | - |
D-ribose 5-phosphate | pH 7.6, 22°C | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | presence of magnesium ions, alone or in combination with thiamine diphosphate, does not affect the activity | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
67900 | - |
x * 68000, SDS-PAGE, x * 67900, calculated | Homo sapiens |
68000 | - |
x * 68000, SDS-PAGE, x * 67900, calculated | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant protein | Homo sapiens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
3.64 | - |
pH 7.6, 22°C | Homo sapiens |
Storage Stability | Organism |
---|---|
-18°C, 50 mM glycylglycine buffer, pH 7.6, no activity loss throughout several months | Homo sapiens |
4°C, 50 mM glycylglycine buffer, pH 7.6, stable for several days | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 D-xylulose 5-phosphate | reversible single substrate reaction, about 6% of the double-substrate reaction rate | Homo sapiens | glyceraldehyde 3-phosphate + glycolaldehyde | - |
? | |
D-xylulose 5-phosphate + D-ribose 5-phosphate | - |
Homo sapiens | sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 68000, SDS-PAGE, x * 67900, calculated | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
thiamine diphosphate | Km value is 0.074 mM | Homo sapiens |