Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | positive transcriptional regulation of the talA-tktB operon by ppGpp | Escherichia coli | |
additional information | positive transcriptional regulation of the talA-tktB operon by ppGpp. Activation of tktB transcription by ppGpp occurs both through the modulation of RpoS levels and independent of RpoS | Escherichia coli |
Application | Comment | Organism |
---|---|---|
additional information | ppGpp-dependent functional pathway that operates through transketolase B, and which is buffered in wild-type strain by the presence of an isozyme, transketolase A | Escherichia coli |
Cloned (Comment) | Organism |
---|---|
construction of plasmid pHR30 using pQE80L vector for IPTG inducible TktB expression | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
additional information | on LB medium, tktB mutants show no growth defect. TktA tktB double mutant shows growth inhibition in LB medium comparable to that observed in tktA ppGpp null strains. DELTAtktB::kan mutation confers synthetic growth defects in the tktA mutant similar to that observed from ppGpp deficiency. PpGpp regulates transketolase B activity in the tktA relA256 mutant | Escherichia coli |
additional information | tktA mutants are slightly slower growing on LB medium. TktA tktB double mutant shows growth inhibition in LB medium comparable to that observed in tktA ppGpp null strains. DELTAtktB::kan mutation confers synthetic growth defects in the tktA mutant similar to that observed from ppGpp deficiency. PpGpp regulates transketolase B activity in the tktA relA256 mutant | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | ppGpp deficiency lowers TktB activity | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P27302 | strain derivatives of MG1655 | - |
Escherichia coli | P33570 | strain derivatives of MG1655 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glyceraldehyde 3-phosphate + D-fructose 6-phosphate | - |
Escherichia coli | D-erythrose 4-phosphate + D-xylulose 5-phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
TktA | - |
Escherichia coli |
TktB | - |
Escherichia coli |
transketolase A | - |
Escherichia coli |
transketolase B | - |
Escherichia coli |