Crystallization (Comment) | Organism |
---|---|
wild-type and mutant E418A in complex with N1-methyl-thiamine diphosphate and wild-type in complex with 4-methylamino-thiamine diphosphate, topology of active sites remains unchanged by cofactor analogues | Saccharomyces cerevisiae |
Protein Variants | Comment | Organism |
---|---|---|
E418A | about 0.12% of wild-type activity | Saccharomyces cerevisiae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4'-methylamino-thiamine diphosphate | cofactor analogue | Saccharomyces cerevisiae | |
N1'-methyl-thiamine diphosphate | cofactor analogue | Saccharomyces cerevisiae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-xylulose 5-phosphate + D-ribose 5-phosphate | - |
Saccharomyces cerevisiae | sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate | - |
? | |
hydroxypyruvate + ferricyanide + H2O | - |
Saccharomyces cerevisiae | glycolic acid + ferrocyanide + ? | - |
? | |
hydroxypyruvate + glycolaldehyde | - |
Saccharomyces cerevisiae | erythrulose + ? | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.002 | - |
Hydroxypyruvate | mutant E418A, cofactor N1-methyl-thiamine diphosphate, 25°C, pH 7.6 | Saccharomyces cerevisiae | |
0.005 | - |
Hydroxypyruvate | wild-type, cofactor N1-methyl-thiamine diphosphate, 25°C, pH 7.6 | Saccharomyces cerevisiae | |
0.05 | - |
D-xylulose 5-phosphate | mutant E418A, cofactor thiamine diphosphate, 25°C, pH 7.6 | Saccharomyces cerevisiae | |
0.63 | - |
D-xylulose 5-phosphate | wild-type, cofactor 4-methylamino-thiamine diphosphate, 25°C, pH 7.6 | Saccharomyces cerevisiae | |
1.8 | - |
D-xylulose 5-phosphate | mutant E418A, cofactor N1-methyl-thiamine diphosphate, 25°C, pH 7.6 | Saccharomyces cerevisiae | |
2.5 | - |
Hydroxypyruvate | wild-type, cofactor 4-methylamino-thiamine diphosphate, 25°C, pH 7.6 | Saccharomyces cerevisiae | |
5 | - |
D-xylulose 5-phosphate | wild-type, cofactor N1-methyl-thiamine diphosphate, 25°C, pH 7.6 | Saccharomyces cerevisiae | |
44.5 | - |
Hydroxypyruvate | wild-type, cofactor thiamine diphosphate, 25°C, pH 7.6 | Saccharomyces cerevisiae | |
45 | - |
D-xylulose 5-phosphate | wild-type, cofactor thiamine diphosphate, 25°C, pH 7.6 | Saccharomyces cerevisiae |