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Literature summary for 2.2.1.1 extracted from

  • Veitch, N.J.; Maugeri, D.A.; Cazzulo, J.J.; Lindqvist, Y.; Barrett, M.P.
    Transketolase from Leishmania mexicana has a dual subcellular localization (2004), Biochem. J., 382, 759-767.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.2.1.1

Cloned(Commentary)

Cloned (Comment) Organism
-
 Leishmania mexicana

Crystallization (Commentary)

Crystallization (Comment) Organism
-
 Leishmania mexicana

Inhibitors

Inhibitors Comment Organism Structure
D-ribose-5-phosphate substrate inhibition above 50 mM Leishmania mexicana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.75
-
 D-ribose-5-phosphate pH 7.5 Leishmania mexicana

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol predominantly cytosolic Leishmania mexicana 5829
-
glycosome significant part of total activity Leishmania mexicana 20015
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
70000
-
 crystallization data, 2 * 70000, SDS-PAGE, 2 * 71800, deduced from gene sequence Leishmania mexicana
71800
-
 crystallization data, 2 * 70000, SDS-PAGE, 2 * 71800, deduced from gene sequence Leishmania mexicana

Organism

Organism UniProt Comment Textmining
Leishmania mexicana Q8MPM3 expression in Escherichia coli
-

Posttranslational Modification

Posttranslational Modification Comment Organism
proteolytic modification type-1 peroxisome targeting signal Leishmania mexicana

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.7
-
 pH 7.5 Leishmania mexicana

Storage Stability

Storage Stability Organism
-80°C, without glycerol, stable Leishmania mexicana

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-xylulose 5-phosphate + D-ribose 5-phosphate
-
 Leishmania mexicana sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate
-
 ?

Subunits

Subunits Comment Organism
dimer crystallization data, 2 * 70000, SDS-PAGE, 2 * 71800, deduced from gene sequence Leishmania mexicana

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
8.4
-
 D-ribose-5-phosphate pH 7.5 Leishmania mexicana