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Literature summary for 2.2.1.1 extracted from
- Purification and characterization of, and preparation of an antibody to, transketolase from human red blood cells (1986), Biochim. Biophys. Acta, 872, 24-32.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| cysteine | activation, 10 mM | Homo sapiens |  |
| ethanol | activation, 1-10% v/v | Homo sapiens | |
General Stability
| General Stability | Organism |
|---|---|
| freezing and thawing, stable to | Homo sapiens |
| purification and storage lead to modification | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Hg2+ | - |
Homo sapiens | |
| PCMB | reversible by cysteine | Homo sapiens | |
| phosphate | not | Homo sapiens | |
| Rabbit antibodies | anti-transketolase IgG, 20% inhibition | Homo sapiens |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.15 | - |
D-xylulose 5-phosphate | (+ D-ribose 5-phosphate) | Homo sapiens | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | no exogenous Mg2+-addition necessary | Homo sapiens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 66000 | - |
alpha2, 2 * 66000, SDS-PAGE | Homo sapiens |
| 104000 | - |
gel filtration | Homo sapiens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| erythrocyte | - |
Homo sapiens | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 3.2 | 3.9 | - |
Homo sapiens |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -90°C, several weeks | Homo sapiens |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | alpha2, 2 * 66000, SDS-PAGE | Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Homo sapiens |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
albumin enhances thermal stability | Homo sapiens |
| 55 | - |
- |
Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| additional information | - |
pI: 8.6 | Homo sapiens |
| 7.6 | 8 | - |
Homo sapiens |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.8 | 9 | about half-maximal activity at pH 6.8 and 9.0 | Homo sapiens |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| thiamine diphosphate | not thiamine, thiamine mono-or triphosphate | Homo sapiens | |
| thiamine diphosphate | additional thiamine does not enhance activity | Homo sapiens | |
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