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Literature summary for 2.2.1.1 extracted from

  • Mocali, A.; Paoletti, F.
    Transketolase from human leukocytes. Isolation, properties and induction of polyclonal antibodies (1989), Eur. J. Biochem., 180, 213-219.
    View publication on PubMed

General Stability

General Stability Organism
dilution, stable to Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.63
-
D-ribose 5-phosphate cosubstrate D-xylulose 5-phosphate Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ requirement Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
66000
-
alpha2, 2 * 66000, SDS-PAGE Homo sapiens
135000
-
gel filtration Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-xylulose 5-phosphate + D-ribose 5-phosphate Homo sapiens
-
sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate
-
r

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
leukocyte
-
Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
10.27
-
-
Homo sapiens

Storage Stability

Storage Stability Organism
-20°C, 2 months Homo sapiens
4°C, several weeks Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-fructose 6-phosphate + D-ribose 5-phosphate 40% as effective as D-xylulose 5-phosphate Homo sapiens ?
-
?
D-xylulose 5-phosphate + D-erythrose 4-phosphate best acceptor with D-xylulose 5-phosphate as donor Homo sapiens D-fructose 6-phosphate + D-glyceraldehyde 3-phosphate
-
r
D-xylulose 5-phosphate + D-ribose 5-phosphate
-
Homo sapiens sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate
-
r
D-xylulose 5-phosphate + D-ribose 5-phosphate best donor with D-ribose 5-phosphate as acceptor Homo sapiens sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate
-
r
D-xylulose 5-phosphate + D-ribose 5-phosphate D-ribose 5-phosphate Homo sapiens sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate
-
r
D-xylulose 5-phosphate + D-ribose 5-phosphate D-erythrose 4-phosphate Homo sapiens sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate
-
r
D-xylulose 5-phosphate + D-ribose 5-phosphate catalyzes reversible transfer of a keto group between several donor and acceptor substrates Homo sapiens sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate
-
r
D-xylulose 5-phosphate + D-ribose 5-phosphate wide specificity for both reactants: donor substrates may be: fructose 6-phosphate Homo sapiens sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate
-
r
additional information no acceptors with D-xylulose 5-phosphate as donor substrate: glucose 6-phosphate Homo sapiens ?
-
?

Subunits

Subunits Comment Organism
dimer alpha2, 2 * 66000, SDS-PAGE Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
additional information
-
pI: 6.5-8.5 (6-8 distinct isozymes, isoelectric focusing) Homo sapiens
8 8.5
-
Homo sapiens

pH Range

pH Minimum pH Maximum Comment Organism
7.1 9.5 about half-maximal activity at pH 7.1 and 9.5 Homo sapiens

Cofactor

Cofactor Comment Organism Structure
thiamine diphosphate
-
Homo sapiens