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Literature summary for 2.2.1.1 extracted from
- The properties from transketolase from photosynthetic tissue (1982), Planta, 155, 316-320.
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| chloroplast | no stimulation by light | Triticum aestivum | 9507 | - |
| chloroplast | no stimulation by light | Spinacia oleracea | 9507 | - |
| cytosol | - |
Triticum aestivum | 5829 | - |
| cytosol | - |
Spinacia oleracea | 5829 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | requirement | Triticum aestivum |  |
| Mg2+ | requirement | Spinacia oleracea | |
| Mg2+ | chloroplastic, not cytosolic enzyme | Triticum aestivum | |
| Mg2+ | chloroplastic, not cytosolic enzyme | Spinacia oleracea | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 37600 | - |
4 * 37600, holoenzyme, SDS-PAGE | Triticum aestivum |
| 150000 | - |
gel filtration | Triticum aestivum |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-xylulose 5-phosphate + D-ribose 5-phosphate | Triticum aestivum | - |
sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate | - |
r | |
| D-xylulose 5-phosphate + D-ribose 5-phosphate | Spinacia oleracea | - |
sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Spinacia oleracea | - |
spinach | - |
| Triticum aestivum | - |
wheat, cv. Sappo | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Triticum aestivum |
- |
Spinacia oleracea |
Renatured (Commentary)
| Renatured (Comment) | Organism |
|---|---|
| thiamine diphosphate reconstitutes dissociated holoenzyme | Triticum aestivum |
| thiamine diphosphate reconstitutes dissociated holoenzyme | Spinacia oleracea |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| leaf | - |
Triticum aestivum | - |
| leaf | - |
Spinacia oleracea | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1.6 | - |
- |
Triticum aestivum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-xylulose 5-phosphate + D-erythrose 4-phosphate | - |
Triticum aestivum | D-fructose 6-phosphate + D-glyceraldehyde 3-phosphate | - |
r | |
| D-xylulose 5-phosphate + D-erythrose 4-phosphate | - |
Spinacia oleracea | D-fructose 6-phosphate + D-glyceraldehyde 3-phosphate | - |
r | |
| D-xylulose 5-phosphate + D-ribose 5-phosphate | - |
Triticum aestivum | sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate | - |
r | |
| D-xylulose 5-phosphate + D-ribose 5-phosphate | - |
Spinacia oleracea | sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate | - |
r | |
| D-xylulose 5-phosphate + D-ribose 5-phosphate | D-erythrose 4-phosphate | Triticum aestivum | sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate | - |
r | |
| D-xylulose 5-phosphate + D-ribose 5-phosphate | D-erythrose 4-phosphate | Spinacia oleracea | sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate | - |
r | |
| D-xylulose 5-phosphate + D-ribose 5-phosphate | sedoheptulose 7-phosphate | Triticum aestivum | sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate | - |
r | |
| D-xylulose 5-phosphate + D-ribose 5-phosphate | sedoheptulose 7-phosphate | Spinacia oleracea | sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate | - |
r | |
| D-xylulose 5-phosphate + D-ribose 5-phosphate | wide specificity for both reactants: donor substrates may be: fructose 6-phosphate | Triticum aestivum | sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate | - |
r | |
| D-xylulose 5-phosphate + D-ribose 5-phosphate | wide specificity for both reactants: donor substrates may be: fructose 6-phosphate | Spinacia oleracea | sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | - |
Spinacia oleracea |
| dimer | apoenzyme, gel filtration after 2-mercaptoethanol incubation | Triticum aestivum |
| More | catalytically active apoenzyme dimer and holoenzyme tetramer | Triticum aestivum |
| More | tetramer dissociates into dimers in the absence of thiamine diphosphate or in dilute solution | Triticum aestivum |
| tetramer | 4 * 37600, holoenzyme, SDS-PAGE | Triticum aestivum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | 8.5 | - |
Triticum aestivum |
| 7.5 | 8.5 | - |
Spinacia oleracea |
| 8.5 | 9 | - |
Triticum aestivum |
| 8.5 | 9 | - |
Spinacia oleracea |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| thiamine diphosphate | - |
Triticum aestivum | |
| thiamine diphosphate | - |
Spinacia oleracea | |
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