Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4-aminobutyrate | - |
Enterococcus faecalis | |
arginine | - |
Enterococcus faecalis | |
arsenate | competitive vs. carbamoylphosphate | Enterococcus faecalis | |
diphosphate | competitive vs. carbamoylphosphate | Enterococcus faecalis | |
glutamate | - |
Enterococcus faecalis | |
guanidobutyrate | - |
Enterococcus faecalis | |
norvaline | - |
Enterococcus faecalis | |
Oxalurate | - |
Enterococcus faecalis | |
phosphate | competitive vs. carbamoylphosphate | Enterococcus faecalis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.02 | - |
putrescine | at pH 7.0 | Enterococcus faecalis | |
0.17 | - |
Carbamoyl phosphate | at pH 7.0 | Enterococcus faecalis | |
4 | - |
arsenate | arsenolytic cleavage | Enterococcus faecalis | |
10 | - |
N-Carbamoylputrescine | arsenolytic cleavage | Enterococcus faecalis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40000 | - |
3 * 40000, treatment with glutaraldehyde, SDS-PAGE | Enterococcus faecalis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Enterococcus faecalis | - |
- |
- |
Pediococcus acidilactici | - |
- |
- |
Purification (Comment) | Organism |
---|---|
65°C, ammonium sulfate, DEAE-Sephadex, Sephadex G-200 | Enterococcus faecalis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
460 | - |
- |
Enterococcus faecalis |
Storage Stability | Organism |
---|---|
-20°C, 2.5 mg/ml, 50 mM potassium phosphate, pH 7.5, 3 years, no loss of activity | Enterococcus faecalis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
arsenate + N-carbamoylputrescine | - |
Enterococcus faecalis | ? | - |
? | |
carbamoyl phosphate + 1,3-diaminopropane | - |
Enterococcus faecalis | phosphate + N1-carbamoyl-1,3-diaminopropane | - |
? | |
carbamoyl phosphate + 1,6-diaminohexane | - |
Enterococcus faecalis | phosphate + N1-carbamoyl-1,6-diaminohexane | - |
? | |
carbamoyl phosphate + cadaverine | - |
Enterococcus faecalis | phosphate + N-carbamoylcadaverine | - |
? | |
carbamoyl phosphate + ornithine | - |
Enterococcus faecalis | phosphate + citrulline | - |
? | |
carbamoyl phosphate + putrescine | - |
Enterococcus faecalis | phosphate + N-carbamoylputrescine | - |
? | |
carbamoyl phosphate + spermidine | - |
Enterococcus faecalis | phosphate + N-carbamoylspermidine | - |
? | |
carbamoyl phosphate + spermine | - |
Enterococcus faecalis | phosphate + N-carbamoylspermine | - |
? |
Subunits | Comment | Organism |
---|---|---|
trimer | 3 * 40000, treatment with glutaraldehyde, SDS-PAGE | Enterococcus faecalis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 9 | at 10 mM putrescine and carbamoyl phosphate | Enterococcus faecalis |
7.8 | - |
at at 0.5 mM putrescine and carbamyol phosphate | Enterococcus faecalis |
9 | - |
at 0.5 mM putrescine | Enterococcus faecalis |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.06 | - |
diphosphate | - |
Enterococcus faecalis | |
4 | - |
phosphate | - |
Enterococcus faecalis | |
12 | - |
arsenate | - |
Enterococcus faecalis |