Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Escherichia coli |
expression in Pseudomonas aeruginosa | Pseudomonas aeruginosa |
Protein Variants | Comment | Organism |
---|---|---|
E106A | mutant arcB6240, with strongly reduced cooperativity for carbamoyl phosphate and anabolic activity | Pseudomonas aeruginosa |
E106G | mutant arcB6254, with strongly reduced cooperativity for carbamoyl phosphate and anabolic activity | Pseudomonas aeruginosa |
Q106E | mutant to study the carbamoyl phosphate cooperativity on the anabolic OTCase | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.06 | - |
Carbamoyl phosphate | Q106E | Escherichia coli | |
0.36 | - |
Carbamoyl phosphate | wild type | Escherichia coli | |
0.8 | - |
ornithine | wild type, pH 7.25 | Pseudomonas aeruginosa | |
0.85 | - |
ornithine | - |
Escherichia coli | |
1.8 | - |
ornithine | mutant arcB6254, pH 7.25 | Pseudomonas aeruginosa | |
1.85 | - |
ornithine | mutant arcB6240, pH 7.25 | Pseudomonas aeruginosa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Pseudomonas aeruginosa | - |
- |
- |
Pseudomonas aeruginosa PAO532 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Pseudomonas aeruginosa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
carbamoyl phosphate + L-ornithine | - |
Escherichia coli | phosphate + citrulline | - |
? | |
carbamoyl phosphate + L-ornithine | - |
Pseudomonas aeruginosa | phosphate + citrulline | - |
? | |
carbamoyl phosphate + L-ornithine | - |
Pseudomonas aeruginosa PAO532 | phosphate + citrulline | - |
? |
Synonyms | Comment | Organism |
---|---|---|
otcase | - |
Escherichia coli |
otcase | - |
Pseudomonas aeruginosa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.25 | - |
wild type | Pseudomonas aeruginosa |
8.2 | - |
mutant arcB6240 | Pseudomonas aeruginosa |
8.5 | - |
mutant arcB6254 | Pseudomonas aeruginosa |