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Literature summary for 2.1.3.3 extracted from

  • Ruepp, A.; Mueller, H.N.; Lottspeich, F.; Soppa, J.
    Catabolic ornithine transcarbamylase of Halobacterium halobium (salinarium): purification, characterization, sequence determination, and evolution (1995), J. Bacteriol., 177, 1129-1136.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.1.3.3

Activating Compound

Activating Compound Comment Organism Structure
arginine 50 mM, 2fold activatin Halobacterium salinarum

Cloned(Commentary)

Cloned (Comment) Organism
-
 Halobacterium salinarum

General Stability

General Stability Organism
unstable below 1 M KCl Halobacterium salinarum

Inhibitors

Inhibitors Comment Organism Structure
ADP 5 mM, 90% inhibition Halobacterium salinarum
AMP 5 mM, 90% inhibition Halobacterium salinarum
ATP 5 mM, 90% inhibition Halobacterium salinarum
CTP 5 mM, 90% inhibition Halobacterium salinarum
GTP 5 mM, 90% inhibition Halobacterium salinarum
phosphate slight inhibition Halobacterium salinarum
UTP 5 mM, 90% inhibition Halobacterium salinarum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
32000
-
 6 * 32000, deduced from nucleotide sequence Halobacterium salinarum
200000
-
 gel filtration Halobacterium salinarum

Organism

Organism UniProt Comment Textmining
Halobacterium salinarum Q48296 also known as Halobacterium salinarium, catabolic ornithine carbamoyltransferase
-

Purification (Commentary)

Purification (Comment) Organism
gel filtration, ammonium sulfate-mediated hydrophobic chromatography Halobacterium salinarum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 highest activity at 4 M KCl Halobacterium salinarum

Storage Stability

Storage Stability Organism
25°C, high-salt solution, 1 year, no loss of activity Halobacterium salinarum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
carbamoyl phosphate + L-ornithine catabolic ornithine carbamoyltransferase, catalyzes the reverse reaction, i.e. the cleavage of citrulline Halobacterium salinarum phosphate + L-citrulline
-
 ?

Subunits

Subunits Comment Organism
hexamer 6 * 32000, deduced from nucleotide sequence Halobacterium salinarum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.8
-
 30% of maximal activity at pH 7.0 Halobacterium salinarum