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Literature summary for 2.1.3.2 extracted from

  • Neelon, K.; Schreier, H.J.; Meekins, H.; Robinson, P.M.; Roberts, M.F.
    Compatible solute effects on thermostability of glutamine synthetase and aspartate transcarbamoylase from Methanococcus jannaschii (2005), Biochim. Biophys. Acta, 1753, 164-173.
    View publication on PubMed

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-
Methanocaldococcus jannaschii
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
carbamoyl phosphate + L-aspartate
-
Escherichia coli phosphate + N-carbamoyl-L-aspartate
-
?
carbamoyl phosphate + L-aspartate
-
Methanocaldococcus jannaschii phosphate + N-carbamoyl-L-aspartate
-
?

Subunits

Subunits Comment Organism
trimer
-
Methanocaldococcus jannaschii

Synonyms

Synonyms Comment Organism
aspartate transcarbamoylase
-
Escherichia coli
aspartate transcarbamoylase
-
Methanocaldococcus jannaschii
ATCase
-
Escherichia coli
ATCase
-
Methanocaldococcus jannaschii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Escherichia coli
55
-
assay at Methanocaldococcus jannaschii

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
55 65 4 min, loss of 70% of secondary structure in absence of solutes, Tm is highly enhanced in presence of KCl, NaCl, or alpha and beta isomers of K+-glutamate, NaCl, KCl, glycine, and betaine are not thermoprotective, but trehalose, sucrose, and K+-glutamate are, K+-glutamate raises Tm and maintains enzyme activity, overview Escherichia coli
95
-
30 min, stable in solutes, but large loss of secondary structure in absence of solutes, the loss is enhanced in presence of KCl, NaCl, or K+-glutamate, glycine and sucrose are thermoprotective Methanocaldococcus jannaschii
100
-
30 min, stable in solutes, but loss of 41% of secondary structure in absence of solutes, the loss is enhanced in presence of KCl, NaCl, or K+-glutamate, glycine and sucrose are thermoprotective Methanocaldococcus jannaschii