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Literature summary for 2.1.3.2 extracted from

  • Chang, T.Y.; Prescott, L.M.; Jones, M.E.
    Aspartate carbamyltransferase (Streptococcus faecalis) (1978), Methods Enzymol., 51, 41-50.
    View publication on PubMed
Search for more literature for 2.1.3.2

Activating Compound

Activating Compound Comment Organism Structure
4-aminobutyrate 400% activation Enterococcus faecalis
beta-Alanine 600% activation Enterococcus faecalis
Butyrate 5700% activation Enterococcus faecalis
Carbamoyl-beta-alanine 5000% activation Enterococcus faecalis
D-Aspartate 5700% activation Enterococcus faecalis
formate activates Enterococcus faecalis
Isobutyrate 5800% activation Enterococcus faecalis
L-2-aminobutyrate 1400% activation Enterococcus faecalis
L-alanine 800% activation Enterococcus faecalis
additional information every type of anion tested, including ATP and CTP can stimulate the reaction to a certain extent Enterococcus faecalis
propionate 5600% activation Enterococcus faecalis

Inhibitors

Inhibitors Comment Organism Structure
HgCl2 0.001 mM, 50% inhibition Enterococcus faecalis
Mersalyl 0.01 mM, 50% inhibition Enterococcus faecalis
p-mercuribenzoate 0.01 mM, 50% inhibition Enterococcus faecalis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.2
-
 Carbamoyl phosphate at high salt concentrations Enterococcus faecalis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
32500
-
 4 * 32500, SDS-PAGE Enterococcus faecalis
125000
-
 sucrose density gradient centrifugation Enterococcus faecalis
128000
-
 sedimentation equilibrium Enterococcus faecalis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
carbamoylphosphate + L-aspartate Enterococcus faecalis
-
 phosphate + N-carbamoyl-L-aspartate
-
 ?

Organism

Organism UniProt Comment Textmining
Enterococcus faecalis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
pH 4.8, hydroxylapatite, ammonium sulfate, DEAE-Sephadex Enterococcus faecalis

Reaction

Reaction Comment Organism Reaction ID
carbamoyl phosphate + L-aspartate = phosphate + N-carbamoyl-L-aspartate reaction mechanism Enterococcus faecalis
a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
350
-
-
 Enterococcus faecalis

Storage Stability

Storage Stability Organism
-20°C, 0.1-1 mg protein/ml, at least 2 years, no loss of activity 4°C, 0.1-1 mg/ml, at least 2 months, no loss of activity Enterococcus faecalis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
carbamoyl phosphate + L-aspartate
-
 Enterococcus faecalis phosphate + N-carbamoyl-L-aspartate
-
 ?
carbamoylphosphate + L-aspartate
-
 Enterococcus faecalis phosphate + N-carbamoyl-L-aspartate
-
 ?

Subunits

Subunits Comment Organism
tetramer 4 * 32500, SDS-PAGE Enterococcus faecalis

pH Stability

pH Stability pH Stability Maximum Comment Organism
6.6
-
 stable Enterococcus faecalis