BRENDA - Enzyme Database show
show all sequences of 2.1.3.12

New aminocoumarin antibiotics formed by a combined mutational and chemoenzymatic approach utilizing the carbamoyltransferase NovN

Xu, H.; Heide, L.; Li, S.M.; Chem. Biol. 11, 655-662 (2004)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
overexpression in Streptomyces lividans as an N-terminal His6 fusion protein
Streptomyces niveus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0019
-
novclobiocin 104
pH 8.0, 30°C
Streptomyces niveus
0.0024
-
decarbamoylnovobiocin
pH 8.0, 30°C
Streptomyces niveus
0.0143
-
novclobiocin 105
pH 8.0, 30°C
Streptomyces niveus
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Mg2+
the enzyme is strictly dependent on the presence ATP and divalent cations such as Mg2+ or Mn2+. Optimal concentrations of both Mg2+ and ATP is 2 mM
Streptomyces niveus
Mn2+
the enzyme is strictly dependent on the presence ATP and divalent cations such as Mg2+ or Mn2+. Optimal concentrations of both Mg2+ and ATP is 2 mM
Streptomyces niveus
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
78500
-
1 * 78500, calculated from sequence
Streptomyces niveus
79000
-
gel filtration
Streptomyces niveus
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
carbamoyl phosphate + decarbamoylnovobiocin
Streptomyces niveus
the enzyme is involved in the biosynthesis of the aminocoumarin antibiotics, novobiocin
phosphate + novobiocin
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Streptomyces niveus
-
-
-
Purification (Commentary)
Commentary
Organism
N-terminal His6 fusion protein
Streptomyces niveus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
carbamoyl phosphate + decarbamoylnovobiocin
the enzyme is involved in the biosynthesis of the aminocoumarin antibiotics, novobiocin
722014
Streptomyces niveus
phosphate + novobiocin
-
-
-
?
carbamoyl phosphate + decarbamoylnovobiocin
NovN is specific for carbamoyl phosphate as substrate. With acetyl phosphate, no product formation is observed
722014
Streptomyces niveus
phosphate + novobiocin
-
-
-
?
carbamoyl phosphate + novclobiocin 104
87% of novclobiocin 104 is converted to the carbamoylated derivative after overnight incubation
722014
Streptomyces niveus
phosphate + ?
-
-
-
?
carbamoyl phosphate + novclobiocin 105
-
722014
Streptomyces niveus
phosphate + novclobiocin 115
-
-
-
?
carbamoyl phosphate + novclobiocin 107
-
722014
Streptomyces niveus
phosphate + novclobiocin 117
-
-
-
?
carbamoyl phosphate + novclobiocin 108
-
722014
Streptomyces niveus
phosphate + novclobiocin 118
-
-
-
?
carbamoyl phosphate + novclobiocin 283
-
722014
Streptomyces niveus
phosphate + novclobiocin 284
-
-
-
?
Subunits
Subunits
Commentary
Organism
monomer
1 * 78500, calculated from sequence
Streptomyces niveus
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
assay at
Streptomyces niveus
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.32
-
novclobiocin 104
pH 8.0, 30°C
Streptomyces niveus
1.53
-
novclobiocin 105
pH 8.0, 30°C
Streptomyces niveus
2.4
-
decarbamoylnovobiocin
pH 8.0, 30°C
Streptomyces niveus
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
assay at
Streptomyces niveus
Cofactor
Cofactor
Commentary
Organism
Structure
ATP
the enzyme is strictly dependent on the presence ATP and divalent cations such as Mg2+ or Mn2+. Optimal concentrations of both Mg2+ and ATP is 2 mM
Streptomyces niveus
Cloned(Commentary) (protein specific)
Commentary
Organism
overexpression in Streptomyces lividans as an N-terminal His6 fusion protein
Streptomyces niveus
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
ATP
the enzyme is strictly dependent on the presence ATP and divalent cations such as Mg2+ or Mn2+. Optimal concentrations of both Mg2+ and ATP is 2 mM
Streptomyces niveus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0019
-
novclobiocin 104
pH 8.0, 30°C
Streptomyces niveus
0.0024
-
decarbamoylnovobiocin
pH 8.0, 30°C
Streptomyces niveus
0.0143
-
novclobiocin 105
pH 8.0, 30°C
Streptomyces niveus
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Mg2+
the enzyme is strictly dependent on the presence ATP and divalent cations such as Mg2+ or Mn2+. Optimal concentrations of both Mg2+ and ATP is 2 mM
Streptomyces niveus
Mn2+
the enzyme is strictly dependent on the presence ATP and divalent cations such as Mg2+ or Mn2+. Optimal concentrations of both Mg2+ and ATP is 2 mM
Streptomyces niveus
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
78500
-
1 * 78500, calculated from sequence
Streptomyces niveus
79000
-
gel filtration
Streptomyces niveus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
carbamoyl phosphate + decarbamoylnovobiocin
Streptomyces niveus
the enzyme is involved in the biosynthesis of the aminocoumarin antibiotics, novobiocin
phosphate + novobiocin
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
N-terminal His6 fusion protein
Streptomyces niveus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
carbamoyl phosphate + decarbamoylnovobiocin
the enzyme is involved in the biosynthesis of the aminocoumarin antibiotics, novobiocin
722014
Streptomyces niveus
phosphate + novobiocin
-
-
-
?
carbamoyl phosphate + decarbamoylnovobiocin
NovN is specific for carbamoyl phosphate as substrate. With acetyl phosphate, no product formation is observed
722014
Streptomyces niveus
phosphate + novobiocin
-
-
-
?
carbamoyl phosphate + novclobiocin 104
87% of novclobiocin 104 is converted to the carbamoylated derivative after overnight incubation
722014
Streptomyces niveus
phosphate + ?
-
-
-
?
carbamoyl phosphate + novclobiocin 105
-
722014
Streptomyces niveus
phosphate + novclobiocin 115
-
-
-
?
carbamoyl phosphate + novclobiocin 107
-
722014
Streptomyces niveus
phosphate + novclobiocin 117
-
-
-
?
carbamoyl phosphate + novclobiocin 108
-
722014
Streptomyces niveus
phosphate + novclobiocin 118
-
-
-
?
carbamoyl phosphate + novclobiocin 283
-
722014
Streptomyces niveus
phosphate + novclobiocin 284
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
monomer
1 * 78500, calculated from sequence
Streptomyces niveus
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
assay at
Streptomyces niveus
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.32
-
novclobiocin 104
pH 8.0, 30°C
Streptomyces niveus
1.53
-
novclobiocin 105
pH 8.0, 30°C
Streptomyces niveus
2.4
-
decarbamoylnovobiocin
pH 8.0, 30°C
Streptomyces niveus
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
assay at
Streptomyces niveus
General Information
General Information
Commentary
Organism
physiological function
the enzyme is involved in the biosynthesis of the aminocoumarin antibiotics, novobiocin
Streptomyces niveus
General Information (protein specific)
General Information
Commentary
Organism
physiological function
the enzyme is involved in the biosynthesis of the aminocoumarin antibiotics, novobiocin
Streptomyces niveus
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
107
-
novclobiocin 105
pH 8.0, 30°C
Streptomyces niveus
695
-
novclobiocin 104
pH 8.0, 30°C
Streptomyces niveus
1000
-
decarbamoylnovobiocin
pH 8.0, 30°C
Streptomyces niveus
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
107
-
novclobiocin 105
pH 8.0, 30°C
Streptomyces niveus
695
-
novclobiocin 104
pH 8.0, 30°C
Streptomyces niveus
1000
-
decarbamoylnovobiocin
pH 8.0, 30°C
Streptomyces niveus
Other publictions for EC 2.1.3.12
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
721183
Gomez Garcia
Crystallization and preliminar ...
Streptomyces niveus
Acta Crystallogr. Sect. F
64
1000-1002
2008
-
-
1
1
-
-
-
-
-
-
1
-
-
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
721284
Freel Meyers
Characterization of NovP and N ...
Streptomyces niveus
Angew. Chem. Int. Ed. Engl.
43
67-70
2004
-
-
1
-
-
-
-
2
-
-
-
1
-
1
-
-
1
-
-
-
-
-
2
-
1
-
-
1
1
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
2
-
-
-
1
-
-
-
1
-
-
-
-
2
-
1
-
-
1
1
-
-
-
-
1
1
-
1
1
721594
Freel Meyers
Assembly of dimeric variants o ...
Streptomyces niveus
Biochemistry
43
15022-15036
2004
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
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-
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-
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-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
722014
Xu
New aminocoumarin antibiotics ...
Streptomyces niveus
Chem. Biol.
11
655-662
2004
-
-
1
-
-
-
-
3
-
2
2
1
-
1
-
-
1
-
-
-
-
-
7
1
1
-
-
3
1
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
3
-
2
2
1
-
-
-
1
-
-
-
-
7
1
1
-
-
3
1
-
-
-
-
1
1
-
3
3