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Literature summary for 2.1.3.1 extracted from

  • Wang, Z.; Lin, M.; Wang, L.; Ammar, E.; Yang, S.
    Metabolic engineering of Propionibacterium freudenreichii subsp. shermanii for enhanced propionic acid fermentation: Effects of overexpressing three biotin-dependent carboxylases (2015), Process Biochem., 50, 194-204.
No PubMed abstract available
Search for more literature for 2.1.3.1

Cloned(Commentary)

Cloned (Comment) Organism
gene mmc, co-overexpression of pyruvate carboxylase, methylmalonyl-CoA decarboxylase, and methylmalonyl-CoA carboxyltransferase from Propionibacterium acidipropionici ATCC 4875 in Propionibacterium shermanii, the MMC activity increases by 107% Propionibacterium freudenreichii

Protein Variants

Protein Variants Comment Organism
additional information metabolic engineering of Propionibacteriumfreudenreichii subsp. shermanii DSM 4902 for enhanced propionic acid fermentation by overexpression of pyruvate carboxylase, methylmalonyl-CoA decarboxylase, and methylmalonyl-CoA carboxyltransferase, three biotin-dependent carboxylases in the dicarboxylic acid pathway controlling the carbon fluxin the Wood–Werkman cycle, from Propionibacterium acidipropionici ATCC 4875 in Propionibacterium shermani. The co-fermentation kinetics show that with more glycerol as carbon source, both the wild-type and Ps(pKPYC4) produce more propionic acid and succinic acid, but acetic acid formation is not as significantly affected, neither is the Gly/Glu ratio, metabolic flux analysis, detailed overview Propionibacterium freudenreichii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(S)-methylmalonyl-CoA + pyruvate Propionibacterium freudenreichii
-
 propanoyl-CoA + oxaloacetate
-
 ?
(S)-methylmalonyl-CoA + pyruvate Propionibacterium freudenreichii DSM 4902
-
 propanoyl-CoA + oxaloacetate
-
 ?

Organism

Organism UniProt Comment Textmining
Propionibacterium freudenreichii
-
 subsp. shermanii, gene mmc
-
Propionibacterium freudenreichii DSM 4902
-
 subsp. shermanii, gene mmc
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-methylmalonyl-CoA + pyruvate
-
 Propionibacterium freudenreichii propanoyl-CoA + oxaloacetate
-
 ?
(S)-methylmalonyl-CoA + pyruvate
-
 Propionibacterium freudenreichii DSM 4902 propanoyl-CoA + oxaloacetate
-
 ?

Synonyms

Synonyms Comment Organism
methylmalonyl-CoA carboxyltransferase
-
 Propionibacterium freudenreichii
MMC
-
 Propionibacterium freudenreichii

General Information

General Information Comment Organism
metabolism the enzyme is one of three biotin-dependent carboxylases in the dicarboxylic acid pathway controlling the carbon fluxin the Wood-Werkman cycle, metabolic flux analysis Propionibacterium freudenreichii