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Literature summary for 2.1.3.1 extracted from

  • Shenoy, B.C.; Xie, Y.; Sha, D.; Samols, D.
    Identification and characterization of a factor which is essential for assembly of transcarboxylase (1993), Biochemistry, 32, 10750-10756.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
1.3S subunit cloned and expressed in Escherichia coli Propionibacterium freudenreichii subsp. shermanii
5S subunit cloned and expressed in Escherichia coli Propionibacterium freudenreichii subsp. shermanii

Crystallization (Commentary)

Crystallization (Comment) Organism
-
Propionibacterium freudenreichii subsp. shermanii

Inhibitors

Inhibitors Comment Organism Structure
additional information loss of assembly-promoting factor activity after digestion with trypsin, V8 protease, pronase Propionibacterium freudenreichii subsp. shermanii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
120000
-
the 26S holoenzyme consists of a hexameric central 12S subunit, 360000 Da and six peripheral dimeric 5S subunits, 6 * 120000 Da each of which is linked to the central subunit by 2 biotinyl 1.3S subunits, 12 * MW 12000 Propionibacterium freudenreichii subsp. shermanii

Organism

Organism UniProt Comment Textmining
Propionibacterium freudenreichii subsp. shermanii
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Propionibacterium freudenreichii subsp. shermanii
2.3
-
-
Propionibacterium freudenreichii subsp. shermanii
43
-
recombinant 5S subunit + assembly-promotin factor, overall reaction Propionibacterium freudenreichii subsp. shermanii
48
-
-
Propionibacterium freudenreichii subsp. shermanii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
propionyl-CoA + oxaloacetate two partial reactions Propionibacterium freudenreichii subsp. shermanii (S)-methylmalonyl-CoA + pyruvate
-
r

Subunits

Subunits Comment Organism
More existence of a novel, previously unidentified subunit, a assemply-promoting factor, APF, which is nessessary for the assemply of enzyme, MW: 60000 Da, SDS-PAGE Propionibacterium freudenreichii subsp. shermanii
More the 26S holoenzyme consists of a hexameric central 12S subunit, 360000 Da and six peripheral dimeric 5S subunits, 6 * 120000 Da each of which is linked to the central subunit by 2 biotinyl 1.3S subunits, 12 * MW 12000 Propionibacterium freudenreichii subsp. shermanii

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
assembly-promoting factor heat stable Propionibacterium freudenreichii subsp. shermanii