General Stability | Organism |
---|---|
alkaline pH, low ionic strength, monovalent ions, low protein concentration and elevated temperatures favor dissociation to inactive 5S, 6S and 1.3S subunits | Propionibacterium sp. |
glycerol, 20% retards dissociation of 12S and 6S-biotinyl subunit | Propionibacterium sp. |
polyvalent anions stabilize | Propionibacterium sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
(S)-methylmalonyl-CoA | - |
Propionibacterium sp. | |
2-oxobutyrate | - |
Propionibacterium sp. | |
3-methyloxaloacetate | strong | Propionibacterium sp. | |
Avidin | strong | Propionibacterium sp. | |
coenzyme A | - |
Propionibacterium sp. | |
additional information | no inhibition by metal chelating agents, such as EDTA | Propionibacterium sp. | |
oxalate | - |
Propionibacterium sp. | |
oxaloacetate | - |
Propionibacterium sp. | |
propionyl pantetheine | - |
Propionibacterium sp. | |
propionyl-CoA | - |
Propionibacterium sp. | |
pyruvate | - |
Propionibacterium sp. | |
SH-reagents | weak | Propionibacterium sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics and mechanism | Propionibacterium sp. | |
0.0077 | - |
(2S)-methylmalonyl-coenzyme A | - |
Propionibacterium sp. | |
0.034 | - |
propionyl-CoA | - |
Propionibacterium sp. | |
0.035 | - |
malonyl-CoA | - |
Propionibacterium sp. | |
0.063 | - |
oxaloacetate | - |
Propionibacterium sp. | |
0.25 | - |
butyryl-CoA | - |
Propionibacterium sp. | |
0.5 | - |
acetyl-CoA | - |
Propionibacterium sp. | |
0.77 | - |
pyruvate | - |
Propionibacterium sp. |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Propionibacterium sp. | 5737 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | requirement | Propionibacterium sp. | |
additional information | no additional metal requirement | Propionibacterium sp. | |
Zn2+ | requirement, can replace Co2+ | Propionibacterium sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
additional information | - |
two active forms of enzyme: 18S and 16S, the 16S species arises from 18S species by loss of a 6S biotin, Co, Zn subunit | Propionibacterium sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
propionyl-CoA + oxaloacetate | Propionibacterium sp. | - |
(S)-methylmalonyl-CoA + pyruvate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Propionibacterium sp. | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Propionibacterium sp. |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
(S)-methylmalonyl-CoA + pyruvate = propanoyl-CoA + oxaloacetate | mechanism | Propionibacterium sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Propionibacterium sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetoacetyl-CoA + oxaloacetate | carboxylation at about 2.5% the rate of propionyl-CoA | Propionibacterium sp. | 3-oxoglutaryl-CoA + pyruvate | - |
? | |
acetyl-CoA + oxaloacetate | carboxylation at about 50% the rate of propionyl-CoA | Propionibacterium sp. | malonyl-CoA + pyruvate | - |
r | |
butyryl-CoA + oxaloacetate | carboxylation at about 10% the rate of propionyl-CoA | Propionibacterium sp. | ethylmalonyl-CoA + pyruvate | - |
? | |
additional information | 2-oxobutyrate, 2-oxovalerate, 2-oxoglutarate, 3-oxoglutarate cannot replace pyruvate and is no C1-donor | Propionibacterium sp. | ? | - |
? | |
propionyl-CoA + oxaloacetate | - |
Propionibacterium sp. | (S)-methylmalonyl-CoA + pyruvate | - |
r | |
propionyl-CoA + oxaloacetate | - |
Propionibacterium sp. | (S)-methylmalonyl-CoA + pyruvate | enzyme specific for the S isomer | r |
Subunits | Comment | Organism |
---|---|---|
More | structure | Propionibacterium sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | 7.8 | broad | Propionibacterium sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
biotin | requirement | Propionibacterium sp. | |
biotin | biotinyl-enzyme, 6 mol per 18S and 4 mol per 16S enzyme species | Propionibacterium sp. | |
additional information | no ATP requirement | Propionibacterium sp. |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000018 | - |
oxalate | - |
Propionibacterium sp. | |
0.00004 | - |
(S)-methylmalonyl-CoA | - |
Propionibacterium sp. | |
0.00049 | - |
propionyl-CoA | - |
Propionibacterium sp. | |
0.0007 | - |
oxaloacetate | - |
Propionibacterium sp. | |
0.003 | - |
3-methyloxaloacetate | - |
Propionibacterium sp. | |
0.003 | 0.007 | propionyl pantetheine | - |
Propionibacterium sp. | |
0.0063 | - |
coenzyme A | - |
Propionibacterium sp. | |
0.0089 | - |
pyruvate | - |
Propionibacterium sp. | |
0.025 | - |
2-oxobutyrate | - |
Propionibacterium sp. |