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Literature summary for 2.1.2.8 extracted from
- T4 phage-coded deoxycytidylate hydroxymethylase: purification and studies on intermolecular interactions (1977), Biochem. Biophys. Res. Commun., 77, 898-904.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| structural gene 42 encodes enzyme | Tequatrovirus T4 |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| additional information | enzyme is no membrane component or very loosely attached to the membrane | Tequatrovirus T4 | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 27000 | - |
2 * 27000, SDS-PAGE | Tequatrovirus T4 |
| 57000 | - |
gel filtration | Tequatrovirus T4 |
| 63000 | - |
sedimentation equilibrium method | Tequatrovirus T4 |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5,10-methylenetetrahydrofolate + H2O + deoxycytidylate | Tequatrovirus T4 | - |
tetrahydrofolate + 5-hydroxymethyldeoxycytidylate | 5-hydroxymethyl-dCMP is a virus-specific DNA precursor | ? |  |
| additional information | Tequatrovirus T4 | enzyme is part of a complex catalyzing DNA precursor biosynthesis | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Tequatrovirus T4 | - |
Escherichia coli B infected with bacteriophage T4D and amber mutants | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Escherichia coli B infected with | Tequatrovirus T4 |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 4.9 | - |
- |
Tequatrovirus T4 |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5,10-methylenetetrahydrofolate + H2O + deoxycytidylate | - |
Tequatrovirus T4 | tetrahydrofolate + 5-hydroxymethyldeoxycytidylate | - |
? | |
| 5,10-methylenetetrahydrofolate + H2O + deoxycytidylate | - |
Tequatrovirus T4 | tetrahydrofolate + 5-hydroxymethyldeoxycytidylate | 5-hydroxymethyl-dCMP is a virus-specific DNA precursor | ? | |
| additional information | enzyme is part of a complex catalyzing DNA precursor biosynthesis | Tequatrovirus T4 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 27000, SDS-PAGE | Tequatrovirus T4 |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| 5,10-methylenetetrahydrofolate | - |
Tequatrovirus T4 | |
| tetrahydrofolate | - |
Tequatrovirus T4 | |
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Release 2023.1 (January 2023)
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