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Literature summary for 2.1.2.8 extracted from
- Virus-induced acquisition of metabolic function. VII. Biosynthesis de novo of deoxycytidylate hydroxymethylase (1964), J. Biol. Chem., 239, 2957-2963.
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 68000 | - |
E. coli B infected with bacteriophage T6r+, gel filtration, sedimentation and diffusion data | bacteriophage T6r+ |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | bacteriophage T6r+ | biosynthesis of enzyme de novo after infection | ? | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| bacteriophage T6r+ | - |
Escherichia coli strain B infected with bacteriophage T6r+ | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Escherichia coli infected with | bacteriophage T6r+ |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
bacteriophage T6r+ |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, up to 10 days, only minor loss of activity | bacteriophage T6r+ |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5,10-methylenetetrahydrofolate + H2O + deoxycytidylate | - |
bacteriophage T6r+ | tetrahydrofolate + 5-hydroxymethyldeoxycytidylate | - |
? | |
| additional information | biosynthesis of enzyme de novo after infection | bacteriophage T6r+ | ? | - |
? | |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | 320 substrate molecules per min per enzyme molecule | bacteriophage T6r+ |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| 5,10-methylenetetrahydrofolate | - |
bacteriophage T6r+ | |
| tetrahydrofolate | - |
bacteriophage T6r+ | |
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