Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli as His-tag fusion protein | Gallus gallus |
Crystallization (Comment) | Organism |
---|---|
recombinant His-tag fusion protein crystallized by sitting drop vapor diffusion method in complex with its inhibitor beta-DADF | Gallus gallus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
beta-DADF | IC50 125 nM | Gallus gallus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
10-formyltetrahydrofolate + 5'-phosphoribosyl-5-amino-imidazole-4-carboxamide | Gallus gallus | penultimate step of the purine de novo synthesis pathway | tetrahydrofolic acid + 5'-phosphoribosyl-5-formamido-4-imidazole-4-carboxamide | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Gallus gallus | P31335 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Gallus gallus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
10-formyltetrahydrofolate + 5'-phosphoribosyl-5-amino-imidazole-4-carboxamide | - |
Gallus gallus | tetrahydrofolic acid + 5'-phosphoribosyl-5-formamido-4-imidazole-4-carboxamide | - |
? | |
10-formyltetrahydrofolate + 5'-phosphoribosyl-5-amino-imidazole-4-carboxamide | penultimate step of the purine de novo synthesis pathway | Gallus gallus | tetrahydrofolic acid + 5'-phosphoribosyl-5-formamido-4-imidazole-4-carboxamide | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | crystal structure analysis, monomer contains two functionally independent domains transformylase and cyclohydrolase activities | Gallus gallus |
Synonyms | Comment | Organism |
---|---|---|
AICAR transformylase/inosine monophosphate cyclohydrolase | bifunctional enzyme catalyzing the last two steps of the purine de novo synthesis pathway | Gallus gallus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
10-formyltetrahydrofolate | - |
Gallus gallus |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.000125 | - |
IC50 125 nM | Gallus gallus | beta-DADF |