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Literature summary for 2.1.2.3 extracted from

  • Wolan, D.W.; Greasley, S.E.; Wall, M.J.; Benkovic, S.J.; Wilson, I.A.
    Structure of avian AICAR transformylase with a multisubstrate adduct inhibitor beta-DADF identifies the folate binding site (2003), Biochemistry, 42, 10904-10914.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli as His-tag fusion protein Gallus gallus

Crystallization (Commentary)

Crystallization (Comment) Organism
recombinant His-tag fusion protein crystallized by sitting drop vapor diffusion method in complex with its inhibitor beta-DADF Gallus gallus

Inhibitors

Inhibitors Comment Organism Structure
beta-DADF IC50 125 nM Gallus gallus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
10-formyltetrahydrofolate + 5'-phosphoribosyl-5-amino-imidazole-4-carboxamide Gallus gallus penultimate step of the purine de novo synthesis pathway tetrahydrofolic acid + 5'-phosphoribosyl-5-formamido-4-imidazole-4-carboxamide
-
?

Organism

Organism UniProt Comment Textmining
Gallus gallus P31335
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Gallus gallus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
10-formyltetrahydrofolate + 5'-phosphoribosyl-5-amino-imidazole-4-carboxamide
-
Gallus gallus tetrahydrofolic acid + 5'-phosphoribosyl-5-formamido-4-imidazole-4-carboxamide
-
?
10-formyltetrahydrofolate + 5'-phosphoribosyl-5-amino-imidazole-4-carboxamide penultimate step of the purine de novo synthesis pathway Gallus gallus tetrahydrofolic acid + 5'-phosphoribosyl-5-formamido-4-imidazole-4-carboxamide
-
?

Subunits

Subunits Comment Organism
dimer crystal structure analysis, monomer contains two functionally independent domains transformylase and cyclohydrolase activities Gallus gallus

Synonyms

Synonyms Comment Organism
AICAR transformylase/inosine monophosphate cyclohydrolase bifunctional enzyme catalyzing the last two steps of the purine de novo synthesis pathway Gallus gallus

Cofactor

Cofactor Comment Organism Structure
10-formyltetrahydrofolate
-
Gallus gallus

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.000125
-
IC50 125 nM Gallus gallus beta-DADF