Application | Comment | Organism |
---|---|---|
pharmacology | enzyme could serve as target for anti-fungal drugs, since it is not present in mammals | Aspergillus nidulans |
Cloned (Comment) | Organism |
---|---|
panB gene encoding enzyme is cloned, sequenced and expressed in Escherichia coli as an active octameric enzyme, ORF encodes a protein of 349 amino acids, panB gene is closely linked to nimO gene on Aspergillus nidulans linkage group VII | Aspergillus nidulans |
Protein Variants | Comment | Organism |
---|---|---|
additional information | panB100 mutant has a deletion of G-268 | Aspergillus nidulans |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
37700 | - |
8 * 37700, calculated from the amino acid sequence, 8 * 40000, recombinant enzyme expressed in Escherichia coli, SDS-PAGE | Aspergillus nidulans |
40000 | - |
8 * 37700, calculated from the amino acid sequence, 8 * 40000, recombinant enzyme expressed in Escherichia coli, SDS-PAGE | Aspergillus nidulans |
340000 | - |
recombinant enzyme expressed in Escherichia coli, gel filtration | Aspergillus nidulans |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,10-methylenetetrahydrofolate + 3-methyl-2-oxobutanoate | Aspergillus nidulans | essential for the biosynthesis of coenzyme A | tetrahydrofolate + 2-dehydropantoate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus nidulans | Q9Y7B6 | wild-type strains biA1 and yA2, strain G1765 | - |
Purification (Comment) | Organism |
---|---|
affinity purification of recombinant PanB | Aspergillus nidulans |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.01 | 0.027 | recombinant enzyme expressed in Escherichia coli | Aspergillus nidulans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,10-methylenetetrahydrofolate + 3-methyl-2-oxobutanoate | - |
Aspergillus nidulans | tetrahydrofolate + 2-dehydropantoate | - |
? | |
5,10-methylenetetrahydrofolate + 3-methyl-2-oxobutanoate | essential for the biosynthesis of coenzyme A | Aspergillus nidulans | tetrahydrofolate + 2-dehydropantoate | - |
? |
Subunits | Comment | Organism |
---|---|---|
octamer | 8 * 37700, calculated from the amino acid sequence, 8 * 40000, recombinant enzyme expressed in Escherichia coli, SDS-PAGE | Aspergillus nidulans |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Aspergillus nidulans |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
5,10-methylenetetrahydrofolate | - |
Aspergillus nidulans | |
tetrahydrofolate | - |
Aspergillus nidulans |