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Literature summary for 2.1.2.11 extracted from

  • Kurtov, D.; Kinghorn, J.R.; Unkles, S.E.
    The Aspergillus nidulans panB gene encodes ketopantoate hydroxymethyltransferase, required for biosynthesis of pantothenate and coenzyme A (1999), Mol. Gen. Genet., 262, 115-120.
    View publication on PubMed

Application

Application Comment Organism
pharmacology enzyme could serve as target for anti-fungal drugs, since it is not present in mammals Aspergillus nidulans

Cloned(Commentary)

Cloned (Comment) Organism
panB gene encoding enzyme is cloned, sequenced and expressed in Escherichia coli as an active octameric enzyme, ORF encodes a protein of 349 amino acids, panB gene is closely linked to nimO gene on Aspergillus nidulans linkage group VII Aspergillus nidulans

Protein Variants

Protein Variants Comment Organism
additional information panB100 mutant has a deletion of G-268 Aspergillus nidulans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
37700
-
8 * 37700, calculated from the amino acid sequence, 8 * 40000, recombinant enzyme expressed in Escherichia coli, SDS-PAGE Aspergillus nidulans
40000
-
8 * 37700, calculated from the amino acid sequence, 8 * 40000, recombinant enzyme expressed in Escherichia coli, SDS-PAGE Aspergillus nidulans
340000
-
recombinant enzyme expressed in Escherichia coli, gel filtration Aspergillus nidulans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5,10-methylenetetrahydrofolate + 3-methyl-2-oxobutanoate Aspergillus nidulans essential for the biosynthesis of coenzyme A tetrahydrofolate + 2-dehydropantoate
-
?

Organism

Organism UniProt Comment Textmining
Aspergillus nidulans Q9Y7B6 wild-type strains biA1 and yA2, strain G1765
-

Purification (Commentary)

Purification (Comment) Organism
affinity purification of recombinant PanB Aspergillus nidulans

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.01 0.027 recombinant enzyme expressed in Escherichia coli Aspergillus nidulans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5,10-methylenetetrahydrofolate + 3-methyl-2-oxobutanoate
-
Aspergillus nidulans tetrahydrofolate + 2-dehydropantoate
-
?
5,10-methylenetetrahydrofolate + 3-methyl-2-oxobutanoate essential for the biosynthesis of coenzyme A Aspergillus nidulans tetrahydrofolate + 2-dehydropantoate
-
?

Subunits

Subunits Comment Organism
octamer 8 * 37700, calculated from the amino acid sequence, 8 * 40000, recombinant enzyme expressed in Escherichia coli, SDS-PAGE Aspergillus nidulans

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Aspergillus nidulans

Cofactor

Cofactor Comment Organism Structure
5,10-methylenetetrahydrofolate
-
Aspergillus nidulans
tetrahydrofolate
-
Aspergillus nidulans