Cloned (Comment) | Organism |
---|---|
panB gene encoding enzyme is cloned, sequenced and overexpressed in Escherichia coli BL21, ORF of 846 bp encodes a protein of 281 amino acids, N-terminal 37 amino acids are essential for enzyme function | Mycobacterium tuberculosis variant bovis |
PCR amplification of panB gene, which is identical to that of Mycobacterium bovis BCG | Mycobacterium tuberculosis |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
soluble | - |
Mycobacterium tuberculosis | - |
- |
soluble | most of recombinant enzyme is found in a soluble fraction | Mycobacterium tuberculosis variant bovis | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
29337 | - |
x * 29337, calculated from the amino acid sequence, x * 35000, recombinant enzyme expressed in Escherichia coli BL21, SDS-PAGE | Mycobacterium tuberculosis variant bovis |
35000 | - |
x * 29337, calculated from the amino acid sequence, x * 35000, recombinant enzyme expressed in Escherichia coli BL21, SDS-PAGE | Mycobacterium tuberculosis variant bovis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,10-methylenetetrahydrofolate + 3-methyl-2-oxobutanoate | Mycobacterium tuberculosis | first committed step in pantothenate biosynthesis | tetrahydrofolate + 2-dehydropantoate | - |
? | |
5,10-methylenetetrahydrofolate + 3-methyl-2-oxobutanoate | Mycobacterium tuberculosis variant bovis | first committed step in pantothenate biosynthesis | tetrahydrofolate + 2-dehydropantoate | - |
? | |
5,10-methylenetetrahydrofolate + 3-methyl-2-oxobutanoate | Mycobacterium tuberculosis KIT 10468 | first committed step in pantothenate biosynthesis | tetrahydrofolate + 2-dehydropantoate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | - |
- |
- |
Mycobacterium tuberculosis KIT 10468 | - |
- |
- |
Mycobacterium tuberculosis variant bovis | - |
BCG, strain 1173-P2, live vaccine against tuberculosis, used for superficial bladder cancer immunotherapy | - |
Purification (Comment) | Organism |
---|---|
purification of recombinant enzyme overexpressed in Escherichia coli BL21 | Mycobacterium tuberculosis variant bovis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.019 | - |
recombinant enzyme | Mycobacterium tuberculosis variant bovis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,10-methylenetetrahydrofolate + 3-methyl-2-oxobutanoate | - |
Mycobacterium tuberculosis | tetrahydrofolate + 2-dehydropantoate | - |
? | |
5,10-methylenetetrahydrofolate + 3-methyl-2-oxobutanoate | - |
Mycobacterium tuberculosis variant bovis | tetrahydrofolate + 2-dehydropantoate | - |
? | |
5,10-methylenetetrahydrofolate + 3-methyl-2-oxobutanoate | first committed step in pantothenate biosynthesis | Mycobacterium tuberculosis | tetrahydrofolate + 2-dehydropantoate | - |
? | |
5,10-methylenetetrahydrofolate + 3-methyl-2-oxobutanoate | first committed step in pantothenate biosynthesis | Mycobacterium tuberculosis variant bovis | tetrahydrofolate + 2-dehydropantoate | - |
? | |
5,10-methylenetetrahydrofolate + 3-methyl-2-oxobutanoate | - |
Mycobacterium tuberculosis KIT 10468 | tetrahydrofolate + 2-dehydropantoate | - |
? | |
5,10-methylenetetrahydrofolate + 3-methyl-2-oxobutanoate | first committed step in pantothenate biosynthesis | Mycobacterium tuberculosis KIT 10468 | tetrahydrofolate + 2-dehydropantoate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 29337, calculated from the amino acid sequence, x * 35000, recombinant enzyme expressed in Escherichia coli BL21, SDS-PAGE | Mycobacterium tuberculosis variant bovis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Mycobacterium tuberculosis |
37 | - |
assay at | Mycobacterium tuberculosis variant bovis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
5,10-methylenetetrahydrofolate | - |
Mycobacterium tuberculosis | |
5,10-methylenetetrahydrofolate | - |
Mycobacterium tuberculosis variant bovis | |
tetrahydrofolate | - |
Mycobacterium tuberculosis | |
tetrahydrofolate | - |
Mycobacterium tuberculosis variant bovis |