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Literature summary for 2.1.2.11 extracted from

  • Kim, J.K.; Kim, K.D.; Lim, J.S.; Lee, H.G.; Kim, S.J.; Cho, S.H.; Jeong, W.H.; Choe, I.S.; Chung, T.W.; Paik, S.G.; Choe, Y.K.
    Cloning and characterization of the Mycobacterium bovis BCG panB gene encoding ketopantoate hydroxymethyltransferase (2001), J. Biochem. Mol. Biol., 34, 342-346.
No PubMed abstract available

Cloned(Commentary)

Cloned (Comment) Organism
panB gene encoding enzyme is cloned, sequenced and overexpressed in Escherichia coli BL21, ORF of 846 bp encodes a protein of 281 amino acids, N-terminal 37 amino acids are essential for enzyme function Mycobacterium tuberculosis variant bovis
PCR amplification of panB gene, which is identical to that of Mycobacterium bovis BCG Mycobacterium tuberculosis

Localization

Localization Comment Organism GeneOntology No. Textmining
soluble
-
Mycobacterium tuberculosis
-
-
soluble most of recombinant enzyme is found in a soluble fraction Mycobacterium tuberculosis variant bovis
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
29337
-
x * 29337, calculated from the amino acid sequence, x * 35000, recombinant enzyme expressed in Escherichia coli BL21, SDS-PAGE Mycobacterium tuberculosis variant bovis
35000
-
x * 29337, calculated from the amino acid sequence, x * 35000, recombinant enzyme expressed in Escherichia coli BL21, SDS-PAGE Mycobacterium tuberculosis variant bovis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5,10-methylenetetrahydrofolate + 3-methyl-2-oxobutanoate Mycobacterium tuberculosis first committed step in pantothenate biosynthesis tetrahydrofolate + 2-dehydropantoate
-
?
5,10-methylenetetrahydrofolate + 3-methyl-2-oxobutanoate Mycobacterium tuberculosis variant bovis first committed step in pantothenate biosynthesis tetrahydrofolate + 2-dehydropantoate
-
?
5,10-methylenetetrahydrofolate + 3-methyl-2-oxobutanoate Mycobacterium tuberculosis KIT 10468 first committed step in pantothenate biosynthesis tetrahydrofolate + 2-dehydropantoate
-
?

Organism

Organism UniProt Comment Textmining
Mycobacterium tuberculosis
-
-
-
Mycobacterium tuberculosis KIT 10468
-
-
-
Mycobacterium tuberculosis variant bovis
-
BCG, strain 1173-P2, live vaccine against tuberculosis, used for superficial bladder cancer immunotherapy
-

Purification (Commentary)

Purification (Comment) Organism
purification of recombinant enzyme overexpressed in Escherichia coli BL21 Mycobacterium tuberculosis variant bovis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.019
-
recombinant enzyme Mycobacterium tuberculosis variant bovis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5,10-methylenetetrahydrofolate + 3-methyl-2-oxobutanoate
-
Mycobacterium tuberculosis tetrahydrofolate + 2-dehydropantoate
-
?
5,10-methylenetetrahydrofolate + 3-methyl-2-oxobutanoate
-
Mycobacterium tuberculosis variant bovis tetrahydrofolate + 2-dehydropantoate
-
?
5,10-methylenetetrahydrofolate + 3-methyl-2-oxobutanoate first committed step in pantothenate biosynthesis Mycobacterium tuberculosis tetrahydrofolate + 2-dehydropantoate
-
?
5,10-methylenetetrahydrofolate + 3-methyl-2-oxobutanoate first committed step in pantothenate biosynthesis Mycobacterium tuberculosis variant bovis tetrahydrofolate + 2-dehydropantoate
-
?
5,10-methylenetetrahydrofolate + 3-methyl-2-oxobutanoate
-
Mycobacterium tuberculosis KIT 10468 tetrahydrofolate + 2-dehydropantoate
-
?
5,10-methylenetetrahydrofolate + 3-methyl-2-oxobutanoate first committed step in pantothenate biosynthesis Mycobacterium tuberculosis KIT 10468 tetrahydrofolate + 2-dehydropantoate
-
?

Subunits

Subunits Comment Organism
? x * 29337, calculated from the amino acid sequence, x * 35000, recombinant enzyme expressed in Escherichia coli BL21, SDS-PAGE Mycobacterium tuberculosis variant bovis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Mycobacterium tuberculosis
37
-
assay at Mycobacterium tuberculosis variant bovis

Cofactor

Cofactor Comment Organism Structure
5,10-methylenetetrahydrofolate
-
Mycobacterium tuberculosis
5,10-methylenetetrahydrofolate
-
Mycobacterium tuberculosis variant bovis
tetrahydrofolate
-
Mycobacterium tuberculosis
tetrahydrofolate
-
Mycobacterium tuberculosis variant bovis