General Stability | Organism |
---|---|
not inactivated by borohydride reduction in the presence of excess substrates | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
coenzyme A | above 1 mM | Escherichia coli | |
formaldehyde | 0.8 mM, partial inhibition; forward reaction | Escherichia coli | |
Pantoate | 0.05 mM or above | Escherichia coli | |
pantothenate | 0.5 mM or above | Escherichia coli | |
tetrahydrofolate | 0.38 mM, partial inhibition; forward reaction | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.18 | - |
tetrahydrofolate | - |
Escherichia coli | |
1.1 | - |
3-methyl-2-oxobutanoate | - |
Escherichia coli | |
5.9 | - |
formaldehyde | - |
Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | requires Mg2+, Mn2+, Co2+ and Zn2+ are progressively less active | Escherichia coli | |
Mg2+ | requires Mg2+, Mn2+, Co2+ and Zn2+ are progressively less active | Escherichia coli | |
Mn2+ | requires Mg2+, Mn2+, Co2+ and Zn2+ are progressively less active | Escherichia coli | |
Zn2+ | requires Mg2+, Mn2+, Co2+ and Zn2+ are progressively less active | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
25700 | - |
10 * 27000, SDS-PAGE, 10 * 25700, amino acid analysis | Escherichia coli |
27000 | - |
10 * 27000, SDS-PAGE, 10 * 25700, amino acid analysis | Escherichia coli |
255000 | - |
sedimentation equilibrium | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,10-methylenetetrahydrofolate + 3-methyl-2-oxobutanoate | Escherichia coli | first committed step in pantothenate biosynthesis | tetrahydrofolate + 2-dehydropantoate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
strain K12, 2450fold purification | Escherichia coli |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
5,10-methylenetetrahydrofolate + 3-methyl-2-oxobutanoate + H2O = tetrahydrofolate + 2-dehydropantoate | class II aldolase | Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
7.3 | - |
- |
Escherichia coli |
Storage Stability | Organism |
---|---|
4°C, 100 mM potassium phosphate, pH 6.8, 1 mM EDTA, 0.5 mM dithiothreitol, 6 months, almost no loss of activity | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,10-methylenetetrahydrofolate + 2-oxobutyrate | - |
Escherichia coli | ? | - |
? | |
5,10-methylenetetrahydrofolate + 2-oxopentanoate | - |
Escherichia coli | ? | - |
? | |
5,10-methylenetetrahydrofolate + 3-methyl-2-oxobutanoate | - |
Escherichia coli | tetrahydrofolate + 2-dehydropantoate | synthesis of ketopantoate, the following components can replace tetrahydrofolate: tetrahydropteroylmono-, di-, tri-, tetra-, penta-, hexa-, and heptaglutamate, absolute requirement for tetrahydrofolate, only the L-isomer is active | r | |
5,10-methylenetetrahydrofolate + 3-methyl-2-oxobutanoate | first committed step in pantothenate biosynthesis | Escherichia coli | tetrahydrofolate + 2-dehydropantoate | - |
? | |
5,10-methylenetetrahydrofolate + 3-methyl-2-oxopentanoate | - |
Escherichia coli | ? | - |
? | |
formaldehyde + tetrahydrofolate | - |
Escherichia coli | methylentetrahydrofolate | - |
? |
Subunits | Comment | Organism |
---|---|---|
decamer | 10 * 27000, SDS-PAGE, 10 * 25700, amino acid analysis | Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
pI: 4.4 | Escherichia coli |
7 | 7.6 | - |
Escherichia coli |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
stable and active over a broad pH-range | Escherichia coli |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
stable and active over a broad pH-range | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
5,10-methylenetetrahydrofolate | - |
Escherichia coli | |
tetrahydrofolate | requires tetrahydrofolate | Escherichia coli |