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Literature summary for 2.1.2.11 extracted from

  • Powers, S.G.; Snell, E.E.
    Purification and properties of ketopantoate hydroxymethyltransferase (1979), Methods Enzymol., 62, 204-209.
    View publication on PubMed

General Stability

General Stability Organism
not inactivated by borohydride reduction in the presence of excess substrates Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
coenzyme A above 1 mM Escherichia coli
formaldehyde 0.8 mM, partial inhibition; forward reaction Escherichia coli
Pantoate 0.05 mM or above Escherichia coli
pantothenate 0.5 mM or above Escherichia coli
tetrahydrofolate 0.38 mM, partial inhibition; forward reaction Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.18
-
tetrahydrofolate
-
Escherichia coli
1.1
-
3-methyl-2-oxobutanoate
-
Escherichia coli
5.9
-
formaldehyde
-
Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ requires Mg2+, Mn2+, Co2+ and Zn2+ are progressively less active Escherichia coli
Mg2+ requires Mg2+, Mn2+, Co2+ and Zn2+ are progressively less active Escherichia coli
Mn2+ requires Mg2+, Mn2+, Co2+ and Zn2+ are progressively less active Escherichia coli
Zn2+ requires Mg2+, Mn2+, Co2+ and Zn2+ are progressively less active Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
25700
-
10 * 27000, SDS-PAGE, 10 * 25700, amino acid analysis Escherichia coli
27000
-
10 * 27000, SDS-PAGE, 10 * 25700, amino acid analysis Escherichia coli
255000
-
sedimentation equilibrium Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5,10-methylenetetrahydrofolate + 3-methyl-2-oxobutanoate Escherichia coli first committed step in pantothenate biosynthesis tetrahydrofolate + 2-dehydropantoate
-
?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
strain K12, 2450fold purification Escherichia coli

Reaction

Reaction Comment Organism Reaction ID
5,10-methylenetetrahydrofolate + 3-methyl-2-oxobutanoate + H2O = tetrahydrofolate + 2-dehydropantoate class II aldolase Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
7.3
-
-
Escherichia coli

Storage Stability

Storage Stability Organism
4°C, 100 mM potassium phosphate, pH 6.8, 1 mM EDTA, 0.5 mM dithiothreitol, 6 months, almost no loss of activity Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5,10-methylenetetrahydrofolate + 2-oxobutyrate
-
Escherichia coli ?
-
?
5,10-methylenetetrahydrofolate + 2-oxopentanoate
-
Escherichia coli ?
-
?
5,10-methylenetetrahydrofolate + 3-methyl-2-oxobutanoate
-
Escherichia coli tetrahydrofolate + 2-dehydropantoate synthesis of ketopantoate, the following components can replace tetrahydrofolate: tetrahydropteroylmono-, di-, tri-, tetra-, penta-, hexa-, and heptaglutamate, absolute requirement for tetrahydrofolate, only the L-isomer is active r
5,10-methylenetetrahydrofolate + 3-methyl-2-oxobutanoate first committed step in pantothenate biosynthesis Escherichia coli tetrahydrofolate + 2-dehydropantoate
-
?
5,10-methylenetetrahydrofolate + 3-methyl-2-oxopentanoate
-
Escherichia coli ?
-
?
formaldehyde + tetrahydrofolate
-
Escherichia coli methylentetrahydrofolate
-
?

Subunits

Subunits Comment Organism
decamer 10 * 27000, SDS-PAGE, 10 * 25700, amino acid analysis Escherichia coli

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
additional information
-
pI: 4.4 Escherichia coli
7 7.6
-
Escherichia coli

pH Range

pH Minimum pH Maximum Comment Organism
additional information
-
stable and active over a broad pH-range Escherichia coli

pH Stability

pH Stability pH Stability Maximum Comment Organism
additional information
-
stable and active over a broad pH-range Escherichia coli

Cofactor

Cofactor Comment Organism Structure
5,10-methylenetetrahydrofolate
-
Escherichia coli
tetrahydrofolate requires tetrahydrofolate Escherichia coli