Literature summary for 2.1.2.10 extracted from

Okamura-Ikeda, K.; Kameoka, N.; Fujiwara, K.; Motokawa, Y.
Probing the H-protein-induced conformational change and the function of the N-terminal region of Escherichia coli T-protein of the glycine cleavage system by limited proteolysis (2003), J. Biol. Chem., 278, 10067-10072.

Search for more literature for 2.1.2.10

Protein Variants

Protein Variants	Comment	Organism
L6A	in contrast to wild-type, quite susceptible to trypsinolysis, 4fold increase in Km-value for reduced H-protein	Escherichia coli
additional information	N-terminal deletion of 7 amino acids, in contrast to wild-type, quite susceptible to trypsinolysis	Escherichia coli
T4A	2fold increase in Km-value for reduced H-protein	Escherichia coli

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.00069	-	reduced H-protein	wild-type with His-tag	Escherichia coli
0.0012	-	reduced H-protein	mutant T4A, His-tagged	Escherichia coli
0.00237	-	reduced H-protein	mutant L6A, His-tagged	Escherichia coli
0.0092	-	5,10-methylenetetrahydropteroyltetraglutamate	wild-type with His-tag	Escherichia coli
0.0108	-	5,10-methylenetetrahydropteroyltetraglutamate	mutant L6A, His-tagged	Escherichia coli
0.0169	-	reduced H-protein	N-terminal deletion mutant, His-tagged	Escherichia coli
0.0174	-	5,10-methylenetetrahydropteroyltetraglutamate	mutant T4A, His-tagged	Escherichia coli
0.0382	-	5,10-methylenetetrahydropteroyltetraglutamate	N-terminal deletion mutant, His-tagged	Escherichia coli
0.0869	-	5,10-methylenetetrahydrofolate	mutant T4A, His-tagged	Escherichia coli
0.0875	-	5,10-methylenetetrahydrofolate	mutant L6A, His-tagged	Escherichia coli
0.0881	-	5,10-methylenetetrahydrofolate	wild-type with His-tag	Escherichia coli
0.151	-	5,10-methylenetetrahydrofolate	N-terminal deletion mutant, His-tagged	Escherichia coli
65.4	-	NH4Cl	wild-type with His-tag	Escherichia coli
78.9	-	NH4Cl	mutant L6A, His-tagged	Escherichia coli
93.5	-	NH4Cl	mutant T4A, His-tagged	Escherichia coli
256	-	NH4Cl	N-terminal deletion mutant, His-tagged	Escherichia coli

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	-	-	-

Reaction

Reaction	Comment	Organism	Reaction ID
[protein]-S8-aminomethyldihydrolipoyllysine + tetrahydrofolate = [protein]-dihydrolipoyllysine + 5,10-methylenetetrahydrofolate + NH3	formation of a folate-binding cavity via the interaction of enzyme with H-protein	Escherichia coli	a

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
reduced H-protein + 5,10-methylene-tetrahydrofolate + NH4Cl	-	Escherichia coli	?	-	?
reduced H-protein + 5,10-methylenetetrahydropteroyltetraglutamate + NH4Cl	-	Escherichia coli	?	-	?

Subunits

Subunits	Comment	Organism
More	interaction of enzyme with H-protein	Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
2.1	-	5,10-methylenetetrahydrofolate	N-terminal deletion mutant, His-tagged	Escherichia coli
2.9	-	reduced H-protein	N-terminal deletion mutant, His-tagged	Escherichia coli
3.9	-	NH4Cl	N-terminal deletion mutant, His-tagged	Escherichia coli
7.8	-	5,10-methylenetetrahydrofolate	mutant T4A, His-tagged	Escherichia coli
8.5	-	reduced H-protein	mutant T4A, His-tagged	Escherichia coli
9.5	-	5,10-methylenetetrahydropteroyltetraglutamate	N-terminal deletion mutant, His-tagged	Escherichia coli
9.6	-	NH4Cl	mutant T4A, His-tagged	Escherichia coli
10.3	-	5,10-methylenetetrahydrofolate	mutant L6A, His-tagged	Escherichia coli
10.5	-	5,10-methylenetetrahydropteroyltetraglutamate	wild-type with His-tag	Escherichia coli
10.6	-	5,10-methylenetetrahydropteroyltetraglutamate	mutant L6A, His-tagged	Escherichia coli
11.6	-	NH4Cl	mutant L6A, His-tagged	Escherichia coli
11.6	-	5,10-methylenetetrahydropteroyltetraglutamate	mutant T4A, His-tagged	Escherichia coli
13.3	-	reduced H-protein	mutant L6A, His-tagged	Escherichia coli
18.4	-	5,10-methylenetetrahydrofolate	wild-type with His-tag	Escherichia coli
19.4	-	reduced H-protein	wild-type with His-tag	Escherichia coli
20.9	-	NH4Cl	wild-type with His-tag	Escherichia coli

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Release 2023.1 (January 2023)