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Literature summary for 2.1.2.10 extracted from

  • Bourguignon, J.; Neuburger, M.; Douce, R.
    Resolution and characterization of the glycine-cleavage reaction in pea leaf mitochondria. Properties of the forward reaction catalysed by glycine decarboxylase and serine hydroxymethyltransferase (1988), Biochem. J., 255, 169-178.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.1.2.10

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrial matrix
-
 Pisum sativum 5759
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45000
-
 gel filtration Pisum sativum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-aminomethyldihydrolipoylprotein + (6S)-tetrahydrofolate Pisum sativum part of the glycine cleavage system dihydrolipoylprotein + (6R)-5,10-methylenetetrahydrofolate + NH3
-
 ?

Organism

Organism UniProt Comment Textmining
Pisum sativum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
8.8fold purification Pisum sativum

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
 Pisum sativum
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.08
-
-
 Pisum sativum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information enzyme is a component of the glycine cleavage system which is composed of P-, H-, L- and T-protein, multienzyme complex Pisum sativum ?
-
 ?
S-aminomethyldihydrolipoylprotein + (6S)-tetrahydrofolate T-protein catalyzes the tetrahydrofolate-dependent step of the glycine cleavage reaction Pisum sativum dihydrolipoylprotein + (6R)-5,10-methylenetetrahydrofolate + NH3
-
 ?
S-aminomethyldihydrolipoylprotein + (6S)-tetrahydrofolate tetrahydrofolate-dependent enzyme Pisum sativum dihydrolipoylprotein + (6R)-5,10-methylenetetrahydrofolate + NH3
-
 ?
S-aminomethyldihydrolipoylprotein + (6S)-tetrahydrofolate part of the glycine cleavage system Pisum sativum dihydrolipoylprotein + (6R)-5,10-methylenetetrahydrofolate + NH3
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 45000, lithium dodecyl sulfate PAGE Pisum sativum

Cofactor

Cofactor Comment Organism Structure
tetrahydrofolate tetrahydrofolate-dependent enzyme Pisum sativum